Fig. 2B | Mitochondrial occupancy (GC total volume) | Normal | Unpaired t test | Pausing vs advancing GC | p = 0.4065 t(17) = 0.8511 |
Fig. 2B’ | Mitochondrial occupancy (GC total area) | Normal | Unpaired t test | Pausing vs advancing GC | p = 0.4310 t(17) = 0.8067 |
Fig. 2C | Mitochondrial occupancy (GC central volume) | Normal | Unpaired t test | Pausing vs advancing GC | p = 0.2829 t(17) = 1.109 |
Fig. 2C’ | Mitochondrial occupancy (GC central area) | Normal | Unpaired t test | Pausing vs advancing GC | p = 0.2325 t(17) = 1.238 |
Fig. 2D | Mitochondrial volume (peripheral volume) | Normal | Unpaired t test | Pausing vs advancing GC | p = 0.4444 t(17) = 0.7831 |
Fig. 2D’ | Number of mitochondria in GC peripheral area | Normal | Unpaired t test | Pausing vs advancing GC | p = 0.7028 t(15) = 0.3889 |
Fig. 2E | Distance from leading edge | Normal | Unpaired t test | Pausing vs advancing GC | p < 0.0001 t(17) = 6.740 |
Fig. 4B | Percentage net mitochondrial transport | Normal | Two-way ANOVA (post hoc Bonferroni) | Pausing vs advancing GC | p = 0.9955 F(1,34) = 0.00003 |
Fig. 4C | Percentage time mitochondria spent mobile | Normal | Unpaired t test | Pausing vs advancing GC | p = 0.0131 t(17) = 2.772 |
Fig. 4C | Percentage time mitochondria spent stationary | Normal | Unpaired t test | Pausing vs advancing GC | p = 0.0075 t(17) = 3.034 |
Fig. 4D | Mitochondrial flux (arriving mitochondria) | Normal | Unpaired t test | Pausing vs advancing GC | p = 0.4666 t(17) = 0.7448 |
Fig. 4D | Mitochondrial flux (leaving mitochondria) | Normal | Unpaired t test | Pausing vs advancing GC | p = 0.3617 t(17) = 0.9374 |
Fig. 4E | Number of arriving mitochondria vs axon growth | Normal | Linear regression | | p = 0.0071 r² = 0.5324 |
Fig. 5D | snpha expression | Normal | Unpaired t test | WT vs lak | p < 0.0001 t(4) = 18.33 |
Fig. 5D | snphb expression | Normal | Unpaired t test | WT vs lak | p < 0.0001 t(4) = 17.84 |
Fig. 6E | Percentage stationary mitochondria | Normal | Unpaired t test | WT vs snph db | p = 0.0074 t(13) = 3.168 |
Fig. 7A | GC total occupancy (percentage volume) | Normal | Unpaired t test | WT vs snph db | p = 0.0023 t(22) = 3.446 |
Fig. 7A’ | GC total occupancy percentage area) | Normal | Unpaired t test | WT vs snph db | p = 0.0178 t(22) = 2.562 |
Fig. 7B | GC central occupancy (percentage volume) | Normal | Unpaired t test | WT vs snph db | p = 0.0184 t(22) = 2.543 |
Fig. 7B’ | GC central occupancy (percentage area) | Normal | Unpaired t test | WT vs snph db | p = 0.0071 t(22) = 2.967 |
Fig. 7C | Peripheral mitochondrial volume | Normal | Unpaired t test | WT vs snph db | p = 0.2765 t(22) = 1.116 |
Fig. 7C’ | Number of mitochondria in peripheral area | Normal | Unpaired t test | WT vs snph db | p = 0.6030 t(21) = 0.5278 |
Fig. 7D | Distance from leading edge (pausing GC) | Normal | Unpaired t test | WT vs snph db | p = 0.0003 t(20) = 4.403 |
Fig. 7D’ | Distance from leading edge (advancing GC) | Normal | Unpaired t test | WT vs snph db | p = 0.1080 t(16) = 1.703 |
Fig. 7E | Mitochondrial flux (arriving mitochondria) | Normal | Unpaired t test | WT vs snph db | p = 0.4976 t(22) = 0.6904 |
Fig. 7E | Mitochondrial flux (leaving mitochondria) | Normal | Unpaired t test | WT vs snph db | p = 0.0412 t(22) = 2.168 |
Fig. 7F | Percentage net mitochondrial transport (anterograde) | Normal | Unpaired t test | WT vs snph db | p = 0.0178 t(22) = 2.561 |
Fig. 7F | Percentage NET mitochondrial transport (retrograde) | Normal | Unpaired t test | WT vs snph db | p = 0.0529 t(22) = 2.046 |
Fig. 7G | Percentage time mitochondria spent in motion (anterograde) | Normal | Unpaired t test | WT vs snph db | p = 0.3330 t(22) = 0.9900 |
Fig. 7G | Percentage time mitochondria spent in motion (retrograde) | Normal | Unpaired t test | WT vs snph db | p = 0.5596 t(22) = 0.5924 |
Fig. 7H | GC total area | Normal | Unpaired t test | WT vs snph db | p = 0.2904 t(22) = 1.083 |
Fig. 7H | GC central area | Normal | Unpaired t test | WT vs snph db | p = 0.3869 t(22) = 0.8829 |
Fig. 7I | Number of filopodia | Normal | Unpaired t test | WT vs snph db | p = 0.6775 t(22) = 0.42154 |
Fig. 7J | Growth rate | Normal | Unpaired t test | WT vs snph db | p = 0.8256 t(15) = 0.2243 |
Fig. 8D | Pioneering cluster area | Normal | Unpaired t test | WT vs snph db | p = 0.0166 t(15) = 2.697 |
Fig. 8E | Number of pioneering clusters | Normal | Unpaired t test | WT vs snph db | p = 0.1865 t(15) = 1.385 |
Fig. 8F | Mitochondria presence (% time) | Normal | Unpaired t test | WT vs snph db | p = 0.1994 t(15) = 1.343 |