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Research ArticleMethods/New Tools, Novel Tools and Methods

Characterization of Nanoscale Organization of F-Actin in Morphologically Distinct Dendritic Spines In Vitro Using Supervised Learning

Siddharth Nanguneri, R. T. Pramod, Nadia Efimova, Debajyoti Das, Mini Jose, Tatyana Svitkina and Deepak Nair
eNeuro 16 July 2019, 6 (4) ENEURO.0425-18.2019; https://doi.org/10.1523/ENEURO.0425-18.2019
Siddharth Nanguneri
1Centre for Neuroscience, Indian Institute of Science, Bangalore, 560012, India
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R. T. Pramod
1Centre for Neuroscience, Indian Institute of Science, Bangalore, 560012, India
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Nadia Efimova
2Department of Biology, University of Pennsylvania, Philadelphia, PA 19104
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Debajyoti Das
1Centre for Neuroscience, Indian Institute of Science, Bangalore, 560012, India
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Mini Jose
1Centre for Neuroscience, Indian Institute of Science, Bangalore, 560012, India
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Tatyana Svitkina
2Department of Biology, University of Pennsylvania, Philadelphia, PA 19104
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Deepak Nair
1Centre for Neuroscience, Indian Institute of Science, Bangalore, 560012, India
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Article Information

DOI 
https://doi.org/10.1523/ENEURO.0425-18.2019
PubMed 
31311803
Published By 
Society for Neuroscience
History 
  • Received October 26, 2018
  • Revision received May 17, 2019
  • Accepted May 22, 2019
  • Published online July 16, 2019.
Copyright & Usage 
Copyright © 2019 Nanguneri et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Siddharth Nanguneri1,
  2. R. T. Pramod1,
  3. Nadia Efimova2,
  4. Debajyoti Das1,
  5. Mini Jose1,
  6. Tatyana Svitkina2 and
  7. Deepak Nair1
  1. 1Centre for Neuroscience, Indian Institute of Science, Bangalore, 560012, India
  2. 2Department of Biology, University of Pennsylvania, Philadelphia, PA 19104
  1. Correspondence should be addressed to Deepak Nair at deepak{at}iisc.ac.in.
View Full Text

Author contributions

  1. Author contributions: S.N., R.T.P., M.J., and D.N. designed the research; S.N., D.D., M.J.,D.N., N.E., and T.S. performed research; N.E. and T.S. contributed unpublished reagents/ analytic tools; S.N., R.T.P., and D.N. analyzed the data; S.N., R.T.P.,T.S., M.J., and D.N. wrote the paper; S.N. and R.T.P. contributed equally for the paper.

Disclosures

  • The authors declare no competing financial interests.

  • This work was supported by Science and Engineering Research Board (Postdoctoral Fellowship PDF/2016/003226 for S.N., Early Career Award ECR/2017/000376 to M.J.), Department of Biotechnology (Ramalingaswami Fellowships to D.N., M.J., Innovative Biotechnologist Award to D.N.), University Grants Commission of India (UGC-IST grant to D.N.), Indian Institute of Science (IISc-DBT Partnership Program ), McGill University and Tata Program Grant (D.N.), and the National Institutes of Health (grant R01 GM095977 to T.S.).

Funding

  • DST | Science and Engineering Research Board (SERB)

    PDF/2016/003226; Fellowship/early career
  • Department of Biotechnology, Ministry of Science and Technology (DBT)

    Ramalingaswami Fellowship; Ramalingaswami Fellowship; Innovative Young Biotechnology Award
  • National institute of Health, USA

    R01 GM095977
  • University Grants Commission (UGC)

    UGC-IST
  • Gouvernement du Canada | Canadian Institutes of Health Research (CIHR)

    McGill Universty Subgrant
  • Tata Trusts

    Tata Program Grant
  • Indian Institute of Science (IISc)

    DBT-IISc

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  • You are viewing the most recent version of this article.
  • previous version (July 16, 2019).

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Total118457011529
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Characterization of Nanoscale Organization of F-Actin in Morphologically Distinct Dendritic Spines In Vitro Using Supervised Learning
Siddharth Nanguneri, R. T. Pramod, Nadia Efimova, Debajyoti Das, Mini Jose, Tatyana Svitkina, Deepak Nair
eNeuro 16 July 2019, 6 (4) ENEURO.0425-18.2019; DOI: 10.1523/ENEURO.0425-18.2019

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Characterization of Nanoscale Organization of F-Actin in Morphologically Distinct Dendritic Spines In Vitro Using Supervised Learning
Siddharth Nanguneri, R. T. Pramod, Nadia Efimova, Debajyoti Das, Mini Jose, Tatyana Svitkina, Deepak Nair
eNeuro 16 July 2019, 6 (4) ENEURO.0425-18.2019; DOI: 10.1523/ENEURO.0425-18.2019
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Keywords

  • Alzheimer’s disease
  • dendritic spines
  • F-actin
  • segmentation and pattern recognition
  • super-resolution
  • supervised learning

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