Figure 1A | Normal distribution | One-way ANOVA | F(6,14) = 52.697, p < 0.0001 |
Vehicle vs 0.01 μM | | Dunnett's multiple comparisons test | CI: –1.8649 to –0.17292 |
Vehicle vs 0.1 μM | | Dunnett's multiple comparisons test | CI: –3.6802 to –1.9882 |
Vehicle vs 1 μM | | Dunnett's multiple comparisons test | CI: –3.9667 to –2.2748 |
Vehicle vs 10 μM | | Dunnett's multiple comparisons test | CI: –3.5352 to –1.8432 |
Vehicle vs FAC | | Dunnett's multiple comparisons test | CI: –4.198 to –2.5061 |
Vehicle vs DFO | | Dunnett's multiple comparisons test | CI: –0.77749 to 0.91446 |
Figure 1B | Normal distribution | One-way ANOVA | F(3,8) = 6.2933, p = 0.0168 |
Vehicle vs morphine | | Dunnett's multiple comparisons test | CI: –1.9825 to –0.01226 |
Vehicle vs PTX | | Dunnett's multiple comparisons test | CI: –1.0416 to 0.92856 |
Vehicle vs PTX + Mor | | Dunnett's multiple comparisons test | CI: –0.53893 to 1.4313 |
Figure 1C | Normal distribution | One-way ANOVA | F(7,16) = 94.711, p < 0.0001 |
Vehicle vs 30 m Mor | | Dunnett's multiple comparisons test | CI: –0.49618 to 1.6318 |
Vehicle vs 6 h Mor | | Dunnett's multiple comparisons test | CI: –1.2963 to 0.8317 |
Vehicle vs 24 h Mor | | Dunnett's multiple comparisons test | CI: –1.6376 to 0.49035 |
Vehicle vs 24 h FAC | | Dunnett's multiple comparisons test | CI: –6.7161 to –4.5881 |
Vehicle vs 1 h TNF | | Dunnett's multiple comparisons test | CI: –2.109 to 0.019 |
Vehicle vs 3 h TNF | | Dunnett's multiple comparisons test | CI: –6.4336 to –4.3057 |
Vehicle vs 24 h TNF | | Dunnett's multiple comparisons test | CI: –0.98676 to 1.1412 |
Figure 1D | Normal distribution | One-way ANOVA | F(3,42) = 0.38357, p = 0.7654 |
Vehicle vs 30 m Mor | | Dunnett's multiple comparisons test | CI: –0.23702 to 0.11747 |
Vehicle vs 6 h Mor | | Dunnett's multiple comparisons test | CI: –0.24894 to 0.10556 |
Vehicle vs 24 h Mor | | Dunnett's multiple comparisons test | CI: –0.21279 to 0.14933 |
Figure 2B, cytoplasmic | Normal distribution | One-way ANOVA | F(3,8) = 24.28, p = 0.0002 |
Vehicle vs 3 h Mor | | Dunnett's multiple comparisons test | CI: –1.002 to 0.4371 |
Vehicle vs 6 h Mor | | Dunnett's multiple comparisons test | CI: –1.629 to –0.1897 |
Vehicle vs 24 h Mor | | Dunnett's multiple comparisons test | CI: –2.684 to –1.245 |
Figure 2B, nuclear | Normal distribution | One-way ANOVA | F(3,8) = 1.644, p = 0.2549 |
Vehicle vs 3 h Mor | | Dunnett's multiple comparisons test | CI: –0.6596 to 0.767 |
Vehicle vs 6 h Mor | | Dunnett's multiple comparisons test | CI: –0.7541 to 0.6725 |
Vehicle vs 24 h Mor | | Dunnett's multiple comparisons test | CI: –1.151 to 0.2751 |
Figure 3A, spine density | Normal distribution | Two-tailed, unpaired t test | t(16) = 9.372, CI: –2.825 to –1.783 |
Figure 3A, spine morphology | Normal distribution | Two-way ANOVA | Interaction F(3,64) = 13.9, p < 0.0001Treatment F(3,64) = 151.9, p < 0.0001Morphology F(1,64) = 81.83, p < 0.0001 |
Vehicle, morphine | | | |
Thin | | Sidak's multiple comparisons test | CI: 0.7779 to 1.428 |
Stubby | | Sidak's multiple comparisons test | CI: 0.007506 to 0.6573 |
Mushroom | | Sidak's multiple comparisons test | CI: 0.4853 to 1.135 |
Filopodia | | Sidak's multiple comparisons test | CI: –0.2768 to 0.373 |
Figure 3B, spine density | Normal distribution | One-way ANOVA | F(4,40) = 50.32, p < 0.0001 |
Vehicle vs 0.01 μM | | Tukey's multiple comparisons test | CI: 0.0951 to 1.616 |
Vehicle vs 0.1 μM | | Tukey's multiple comparisons test | CI: 1.048 to 2.569 |
Vehicle vs 1 μM | | Tukey's multiple comparisons test | CI: 2.003 to 3.524 |
Vehicle vs 10 μM | | Tukey's multiple comparisons test | CI: 2.487 to 4.008 |
0.01 vs 0.1 μM | | Tukey's multiple comparisons test | CI: 0.1923 to 1.713 |
0.1 vs 1 μM | | Tukey's multiple comparisons test | CI: 0.1951 to 1.716 |
1 vs 10 μM | | Tukey's multiple comparisons test | CI: –0.2771 to 1.244 |
Figure 3B, spine morphology | Normal distribution | Two-way ANOVA | Interaction F(12,160) = 21.58, p < 0.0001Morphology F(3,160) = 956.9, p < 0.0001Treatment F(4,160) = 42.9, p < 0.0001 |
Filopodia | | | |
Vehicle vs 0.01 μM | | Tukey's multiple comparisons test | CI: –0.4073 to 0.3795 |
Vehicle vs 0.1 μM | | Tukey's multiple comparisons test | CI: –0.1906 to 0.5962 |
Vehicle vs 1 μM | | Tukey's multiple comparisons test | CI: –0.3156 to 0.4712 |
Vehicle vs 10 μM | | Tukey's multiple comparisons test | CI: –0.2767 to 0.5101 |
Mushroom | | | |
Vehicle vs 0.01 μM | | Tukey's multiple comparisons test | CI: –0.1934 to 0.5934 |
Vehicle vs 0.1 μM | | Tukey's multiple comparisons test | CI: 0.006604 to 0.7934 |
Vehicle vs 1 μM | | Tukey's multiple comparisons test | CI: 0.08438 to 0.8712 |
Vehicle vs 10 μM | | Tukey's multiple comparisons test | CI: 0.1983 to 0.9851 |
|
Stubby | | | |
Vehicle vs 0.01 μM | | Tukey's multiple comparisons test | CI: –0.3934 to 0.3934 |
Vehicle vs 0.1 μM | | Tukey's multiple comparisons test | CI: –0.4378 to 0.349 |
Vehicle vs 1 μM | | Tukey's multiple comparisons test | CI: –0.3434 to 0.4434 |
Vehicle vs 10 μM | | Tukey's multiple comparisons test | CI: –0.3295 to 0.4573 |
Thin | | | |
Vehicle vs 0.01 μM | | Tukey's multiple comparisons test | CI: 0.4372 to 1.224 |
Vehicle vs 0.1 μM | | Tukey's multiple comparisons test | CI: 0.803 to 1.59 |
Vehicle vs 1 μM | | Tukey's multiple comparisons test | CI: 1.77 to 2.557 |
Vehicle vs 10 μM | | Tukey's multiple comparisons test | CI: 2.114 to 2.9 |
0.01 vs 0.1 μM | | Tukey's multiple comparisons test | CI: –0.02756 to 0.7592 |
0.1 vs 1 μM | | Tukey's multiple comparisons test | CI: 0.5741 to 1.361 |
1 vs 10 μM | | Tukey's multiple comparisons test | CI: –0.05034 to 0.7365 |
Figure 3C, spine density | Normal distribution | One-way ANOVA | F(5,42) = 15.29, p < 0.0001 |
Vehicle vs morphine | | Tukey's multiple comparisons test | CI: 1.608 to 3.41 |
Vehicle vs PTX | | Tukey's multiple comparisons test | CI: 0.0146 to 1.817 |
Vehicle vs PTX + Mor | | Tukey's multiple comparisons test | CI: –0.3166 to 1.485 |
Vehicle vs CTAP | | Tukey's multiple comparisons test | CI: 0.2709 to 2.073 |
Vehicle vs CTAP + Mor | | Tukey's multiple comparisons test | CI: 0.09273 to 1.895 |
PTX vs PTX + Mor | | Tukey's multiple comparisons test | CI: –1.232 to 0.5698 |
CTAP vs CTAP + Mor | | Tukey's multiple comparisons test | CI: –1.079 to 0.7229 |
Figure 3C, spine morphology | Normal distribution | Two-way ANOVA | Interaction F(15,168) = 9.193, p < 0.0001Morphology F(3,168) = 1448, p < 0.0001Treatment F(5,168) = 17.39, p < 0.0001 |
Filopodia | | | |
Vehicle vs morphine | | Tukey's multiple comparisons test | CI: –0.3425 to 0.4612 |
Vehicle vs PTX | | Tukey's multiple comparisons test | CI: –0.3894 to 0.4144 |
Vehicle vs PTX + Mor | | Tukey's multiple comparisons test | CI: –0.4519 to 0.3519 |
Vehicle vs CTAP | | Tukey's multiple comparisons test | CI: –0.3675 to 0.4362 |
Vehicle vs CTAP + Mor | | Tukey's multiple comparisons test | CI: –0.3487 to 0.455 |
Mushroom | | | |
Vehicle vs morphine | | Tukey's multiple comparisons test | CI: 0.1544 to 0.9581 |
Vehicle vs PTX | | Tukey's multiple comparisons test | CI: –0.2019 to 0.6019 |
Vehicle vs PTX + Mor | | Tukey's multiple comparisons test | CI: –0.2956 to 0.5081 |
Vehicle vs CTAP | | Tukey's multiple comparisons test | CI: –0.08937 to 0.7144 |
Vehicle vs CTAP + Mor | | Tukey's multiple comparisons test | CI: –0.1081 to 0.6956 |
PTX vs PTX + Mor | | Tukey's multiple comparisons test | CI: –0.4956 to 0.3081 |
CTAP vs CTAP + Mor | | Tukey's multiple comparisons test | CI: –0.4206 to 0.3831 |
Stubby | | | |
Vehicle vs morphine | | Tukey's multiple comparisons test | CI: –0.4644 to 0.3394 |
Vehicle vs PTX | | Tukey's multiple comparisons test | CI: –0.5612 to 0.2425 |
Vehicle vs PTX + Mor | | Tukey's multiple comparisons test | CI: –0.4206 to 0.3831 |
Vehicle vs CTAP | | Tukey's multiple comparisons test | CI: –0.4144 to 0.3894 |
Vehicle vs CTAP + Mor | | Tukey's multiple comparisons test | CI: –0.38 to 0.4237 |
Thin | | | |
Vehicle vs morphine | | Tukey's multiple comparisons test | CI: 1.404 to 2.208 |
Vehicle vs PTX | | Tukey's multiple comparisons test | CI: 0.1481 to 0.9519 |
Vehicle vs PTX + Mor | | Tukey's multiple comparisons test | CI: –0.03 to 0.7737 |
Vehicle vs CTAP | | Tukey's multiple comparisons test | CI: 0.4544 to 1.258 |
Vehicle vs CTAP + Mor | | Tukey's multiple comparisons test | CI: –0.03625 to 0.7675 |
PTX vs PTX + Mor | | Tukey's multiple comparisons test | CI: –0.58 to 0.2237 |
CTAP vs CTAP + Mor | | Tukey's multiple comparisons test | CI: –0.8925 to –0.08875 |
Figure 4A | Normal distribution | Two-tailed, unpaired t test | t(6) = 2.717, CI: 0.6332 to 12.11 |
Figure 4B, spine density | Normal distribution | Two-tailed, unpaired t test | t(10) = 8.482, CI: –7.026 to –4.103 |
Figure 4B, spine morphology | Normal distribution | Two-way ANOVA | Interaction F(3,40) = 7.579, p = 0.0004Morphology F(3,40) = 114, p < 0.0001Treatment F(1,40) = 44.5, p < 0.0001 |
Vehicle, morphine | | | |
Thin | | Sidak's multiple comparisons test | CI: 1.922 to 4.195 |
Stubby | | Sidak's multiple comparisons test | CI: –0.2348 to 2.038 |
Mushroom | | Sidak's multiple comparisons test | CI: 0.4543 to 2.727 |
|
Filopodia | | Sidak's multiple comparisons test | CI: –0.8738 to 1.399 |
Figure 5A | Normal distribution | Two-way ANOVA | Interaction F(4,20) = 0.1919, p = 0.9398Treatment F(4,20) = 12.94, p < 0.0001Expression F(1,20) = 0.002895, p = 0.9576 |
Vehicle:FHC vs 30 m FAC:FHC | | Tukey's multiple comparisons test | CI: –1.704 to 1.063 |
Vehicle:FHC vs 6 h FAC:FHC | | Tukey's multiple comparisons test | CI: –1.8 to 0.9668 |
Vehicle:FHC vs 24 h FAC:FHC | | Tukey's multiple comparisons test | CI: –2.94 to –0.1729 |
Vehicle:FHC vs DFO:FHC | | Tukey's multiple comparisons test | CI: –1.299 to 1.468 |
Vehicle:FLC vs 30 m FAC:FLC | | Tukey's multiple comparisons test | CI: –1.433 to 1.334 |
Vehicle:FLC vs 6 h FAC:FLC | | Tukey's multiple comparisons test | CI: –1.762 to 1.005 |
Vehicle:FLC vs 24 h FAC:FLC | | Tukey's multiple comparisons test | CI: –3.136 to –0.3684 |
Vehicle:FLC vs DFO:FLC | | Tukey's multiple comparisons test | CI: –1.365 to 1.402 |
24 h FAC:FHC vs 24 h FAC:FLC | | Tukey's multiple comparisons test | CI: –1.579 to 1.188 |
Figure 5B | Normal distribution | Two-way ANOVA | Interaction F(3,24) = 1.244, p = 0.3157Treatment F(3,24) = 22.94, p < 0.0001Expression F(1,24) = 9.252, p = 0.0056 |
Vehicle:FHC vs 30 m Mor:FHC | | Sidak's multiple comparisons test | CI: –1.581 to 0.5878 |
Vehicle:FHC vs 6 h Mor:FHC | | Sidak's multiple comparisons test | CI: –2.202 to –0.03317 |
Vehicle:FHC vs 24 h Mor:FHC | | Sidak's multiple comparisons test | CI: –3.17 to –1.001 |
Vehicle:FLC vs 30 m Mor:FLC | | Sidak's multiple comparisons test | CI: –1.118 to 1.051 |
Vehicle:FLC vs 6 h Mor:FLC | | Sidak's multiple comparisons test | CI: –1.596 to 0.5731 |
Vehicle:FLC vs 24 h Mor:FLC | | Sidak's multiple comparisons test | CI: –2.356 to –0.1869 |
30 m Mor:FHC vs 30 m Mor:FLC | | Sidak's multiple comparisons test | CI: –0.6212 to 1.548 |
6 h Mor:FHC vs 6 h Mor:FLC | | Sidak's multiple comparisons test | CI: –0.4783 to 1.691 |
24 h Mor:FHC vs 24 h Mor:FLC | | Sidak's multiple comparisons test | CI: –0.2706 to 1.899 |
Figure 5C | Normal distribution | Two-way ANOVA | Interaction F(1,12) = 3.823, p = 0.0742Treatment F(1,12) = 43.94, p < 0.0001Expression F(1,12) = 3.814, p = 0.0745 |
Vehicle:FHC vs morphine:FHC | | Tukey's multiple comparisons test | CI: –1.358 to –0.4659 |
Vehicle:FLC vs morphine:FHC | | Tukey's multiple comparisons test | CI: –1.358 to –0.4657 |
Morphine:FHC vs morphine:FLC | | Tukey's multiple comparisons test | CI: –0.03085 to 0.8613 |
Figure 6B | Normal distribution | One-way ANOVA | F(4,8) = 112, p < 0.0001 |
Vehicle vs 0.1 μM | | Dunnett's multiple comparisons test | CI: –1.472 to 2.722 |
Vehicle vs 1 μM | | Dunnett's multiple comparisons test | CI: 1.293 to 5.487 |
Vehicle vs 10 μM | | Dunnett's multiple comparisons test | CI: 8.804 to 13.49 |
Vehicle vs 100 μM | | Dunnett's multiple comparisons test | CI: 10.07 to 14.76 |
Figure 6D | Normal distribution | One-way ANOVA | F(4,13) = 47.98, p < 0.0001 |
Vehicle vs 0.1 μM | | Dunnett's multiple comparisons test | CI: –26.25 to 4.68 |
Vehicle vs 1 μM | | Dunnett's multiple comparisons test | CI: –34.28 to –6.624 |
Vehicle vs 10 μM | | Dunnett's multiple comparisons test | CI: –60.4 to –35.66 |
Vehicle vs 100 μM | | Dunnett's multiple comparisons test | CI: –61.63 to –36.89 |
Figure 6E, top | Normal distribution | One-way ANOVA | F(3,8) = 180.2, p < 0.0001 |
Vehicle vs 0.1 μM | | Dunnett's multiple comparisons test | CI: –0.2068 to 0.0702 |
Vehicle vs 1 μM | | Dunnett's multiple comparisons test | CI: –0.6368 to –0.3598 |
Vehicle vs 10 μM | | Dunnett's multiple comparisons test | CI: –1.127 to –0.8498 |
Figure 6E, bottom | Normal distribution | One-way ANOVA | F(3,8) = 127.2, p < 0.0001 |
Vehicle vs morphine | | Dunnett's multiple comparisons test | CI: –1.17 to –0.8066 |
Vehicle vs naloxone | | Dunnett's multiple comparisons test | CI: –0.11 to 0.2534 |
Vehicle vs Nal + Mor | | Dunnett's multiple comparisons test | CI: –0.21 to 0.1534 |
Figure 6G, EL | Normal distribution | Two-tailed, unpaired t test | t(4) = 7.036, CI: –27.35 to –11.87 |
Figure 6G, cytosol | Normal distribution | Two-tailed, unpaired t test | t(4) = 16.86, CI: 11.13 to 15.52 |
Figure 6H, EL | Normal distribution | One-way ANOVA | F(3,8) = 541.2, p < 0.0001 |
Vehicle vs morphine | | Tukey's multiple comparisons test | CI: 30.67 to 36.88 |
Vehicle vs naloxone | | Tukey's multiple comparisons test | CI: –2.663 to 3.55 |
Vehicle vs Nal + Mor | | Tukey's multiple comparisons test | CI: 4.1 to 10.31 |
Morphine vs Nal + Mor | | Tukey's multiple comparisons test | CI: –29.68 to –23.46 |
Figure 6H, cytosol | Normal distribution | One-way ANOVA | F(3,8) = 26.27, p = 0.0002 |
Vehicle vs morphine | | Tukey's multiple comparisons test | CI: –14.9 to –5.351 |
Vehicle vs DFO | | Tukey's multiple comparisons test | CI: –4.623 to 4.929 |
Vehicle vs DFO + Mor | | Tukey's multiple comparisons test | CI: –3.169 to 6.383 |
Morphine vs DFO + Mor | | Tukey's multiple comparisons test | CI: 6.957 to 16.51 |
|
Figure 6I | Normal distribution | One-way ANOVA | F(3,8) = 45.05, p < 0.0001 |
Vehicle vs morphine | | Tukey's multiple comparisons test | CI: –0.249 to –0.131 |
Vehicle vs naloxone | | Tukey's multiple comparisons test | CI: –0.06895 to 0.04895 |
Vehicle vs Nal + Mor | | Tukey's multiple comparisons test | CI: –0.119 to –0.001048 |
Morphine vs Nal + Mor | | Tukey's multiple comparisons test | CI: 0.07105 to 0.189 |
Figure 7A | Normal distribution | One-way ANOVA | F(7,16) = 87.91, p < 0.0001 |
Vehicle vs 30 m Mor | | Dunnett's multiple comparisons test | CI: –678.2 to –19.94 |
Vehicle vs 3 h Mor | | Dunnett's multiple comparisons test | CI: –1010 to –351.8 |
Vehicle vs 6 h Mor | | Dunnett's multiple comparisons test | CI: –1033 to –374.9 |
Vehicle vs 24 h Mor | | Dunnett's multiple comparisons test | CI: –741.5 to –83.21 |
Vehicle vs Phen | | Dunnett's multiple comparisons test | CI: –27.91 to 630.4 |
Vehicle vs Phen + 24 h Mor | | Dunnett's multiple comparisons test | CI: –208.3 to 449.9 |
Vehicle vs FAC | | Dunnett's multiple comparisons test | CI: –2417 to –1759 |
Figure 7B | Normal distribution | One-way ANOVA | F(5,14) = 13.72, p < 0.0001 |
Vehicle vs morphine | | Dunnett's multiple comparisons test | CI: –0.954 to –0.06 |
Vehicle vs DFO | | Dunnett's multiple comparisons test | CI: –0.06606 to 0.8279 |
Vehicle vs Mor + DFO | | Dunnett's multiple comparisons test | CI: –0.009428 to 0.8182 |
Vehicle vs DTPA | | Dunnett's multiple comparisons test | CI: –0.1879 to 0.7061 |
Vehicle vs Mor + DTPA | | Dunnett's multiple comparisons test | CI: –0.9824 to –0.08839 |
Figure 7C, spine density | Normal distribution | One-way ANOVA | F(6,56) = 24.21, p < 0.0001 |
Vehicle vs morphine | | Tukey's multiple comparisons test | CI: 1.869 to 3.642 |
Vehicle vs FAC | | Tukey's multiple comparisons test | CI: 1.494 to 3.267 |
Vehicle vs DFO | | Tukey's multiple comparisons test | CI: 0.1525 to 1.925 |
Vehicle vs DFO + Mor | | Tukey's multiple comparisons test | CI: 0.1108 to 1.884 |
Vehicle vs DTPA | | Tukey's multiple comparisons test | CI: –0.3058 to 1.467 |
Vehicle vs DTPA + Mor | | Tukey's multiple comparisons test | CI: 1.13 to 2.903 |
Morphine vs FAC | | Tukey's multiple comparisons test | CI: –1.261 to 0.5114 |
DFO vs DFO + Mor | | Tukey's multiple comparisons test | CI: –0.928 to 0.8447 |
DTPA vs DTPA + Mor | | Tukey's multiple comparisons test | CI: 0.5497 to 2.322 |
Figure 7C, spine morphology | Normal distribution | Two-way ANOVA | Interaction F(18,224) = 17.58Morphology F(3,224) = 1991, p < 0.0001Treatment F(6,224) = 31.61, p < 0.0001 |
Filopodia | | | |
Vehicle vs morphine | | Tukey's multiple comparisons test | CI: –0.254 to 0.504 |
Vehicle vs FAC | | Tukey's multiple comparisons test | CI: –0.2623 to 0.4956 |
Vehicle vs DFO | | Tukey's multiple comparisons test | CI: –0.2984 to 0.4595 |
Vehicle vs DFO + Mor | | Tukey's multiple comparisons test | CI: –0.3355 to 0.4225 |
Vehicle vs DTPA | | Tukey's multiple comparisons test | CI: –0.2568 to 0.5012 |
Vehicle vs DTPA + Mor | | Tukey's multiple comparisons test | CI: –0.2512 to 0.5068 |
Mushroom | | | |
Vehicle vs morphine | | Tukey's multiple comparisons test | CI: 0.1044 to 0.8623 |
Vehicle vs FAC | | Tukey's multiple comparisons test | CI: 0.07103 to 0.829 |
Vehicle vs DFO | | Tukey's multiple comparisons test | CI: –0.1206 to 0.6373 |
Vehicle vs DFO + Mor | | Tukey's multiple comparisons test | CI: –0.05953 to 0.6984 |
Vehicle vs DTPA | | Tukey's multiple comparisons test | CI: –0.2068 to 0.5512 |
Vehicle vs DTPA + Mor | | Tukey's multiple comparisons test | CI: 0.03769 to 0.7956 |
Morphine vs FAC | | Tukey's multiple comparisons test | CI: –0.4123 to 0.3456 |
DFO vs DFO + Mor | | Tukey's multiple comparisons test | CI: –0.3179 to 0.4401 |
DTPA vs DTPA + Mor | | Tukey's multiple comparisons test | CI: –0.1345 to 0.6234 |
Stubby | | | |
Vehicle vs morphine | | Tukey's multiple comparisons test | CI: –0.3845 to 0.3734 |
Vehicle vs FAC | | Tukey's multiple comparisons test | CI: –0.3706 to 0.3873 |
Vehicle vs DFO | | Tukey's multiple comparisons test | CI: –0.4429 to 0.3151 |
Vehicle vs DFO + Mor | | Tukey's multiple comparisons test | CI: –0.3956 to 0.3623 |
Vehicle vs DTPA | | Tukey's multiple comparisons test | CI: –0.3956 to 0.3623 |
Vehicle vs DTPA + Mor | | Tukey's multiple comparisons test | CI: –0.3929 to 0.3651 |
Thin | | | |
Vehicle vs morphine | | Tukey's multiple comparisons test | CI: 1.788 to 2.546 |
Vehicle vs FAC | | Tukey's multiple comparisons test | CI: 1.457 to 2.215 |
Vehicle vs DFO | | Tukey's multiple comparisons test | CI: 0.4081 to 1.166 |
Vehicle vs DFO + Mor | | Tukey's multiple comparisons test | CI: 0.3432 to 1.101 |
Vehicle vs DTPA | | Tukey's multiple comparisons test | CI: –0.07897 to 0.679 |
|
Vehicle vs DTPA + Mor | | Tukey's multiple comparisons test | CI: 1.121 to 1.879 |
Morphine vs FAC | | Tukey's multiple comparisons test | CI: –0.7095 to 0.04842 |
DFO vs DFO + Mor | | Tukey's multiple comparisons test | CI: –0.4438 to 0.3142 |
DTPA vs DTPA + Mor | | Tukey's multiple comparisons test | CI: 0.821 to 1.579 |