Article Figures & Data
Figures
- Figure 1.
RADs for high-throughput longitudinal recording of rodent physical activity. A, Schematic showing location of assembled RAD in rodent home cage. PIR sensor is activated by animal locomotion. B, Diagram of PIR sensor (MSP430-PIR, Olimex), SD logger (M0 AdaLogger, Adafruit #2796), and organic LED shield (FeatherWing, AdaFruit #2900) connected to a 3.7-V lithium ion battery (Adafruit #353). Components are soldered and (C) housed in a 3D-printed casing (for Arduino code and 3D printing files, see https://hackaday.io/project/160742homecage-activity-monitoring-with-pirs) and fitted on top of the chow tray, which is then placed in the cage (D). D, Top and side views of RAD fitted onto the chow try and placed in the home cage. E, Green “sensor zone” showing where RAD monitors activity in the home cage.
- Figure 2.
Data recorded by RAD correlates with speed. A, Diagram showing setup of 24-h video-tracking PIR validation experiment in Noldus phenotyper. B, Scatter plot of PIR sensor binned data versus video-tracked distance moved for bins of 60 s showing strong correlation between PIR sensor binned data and tracked mouse speed; R 2 = 0.8651, N = 4 mice, data from each mouse are shaded separately. Diagrams showing setups of home cage video-tracking validation for top (C) and side (E) RAD locations. Scatterplots show correlations between PIR active bouts per minute and distance moved per minute for top-setup (D) and side-setup (F); R 2 = 0.6617 and R 2 = 0.7357 for top and side setup, respectively; N = 3 mice, data from each mouse are shaded separately.
- Figure 3.
RAD records continuous activity. Traces show average activity over time (active seconds per minute) for eight individual mice (A) and pooled from n = 40 mice (B, C) for 3 d (B). Scatterplots (C) showing average activity during the day (light cycle) and night (dark cycle) for 40 mice, as well as calculated circadian indices (D), illustrate heterogeneity across the sample population. Data in B are presented in mean activity (dark line) ± SEM (shaded lines).
- Figure 4.
Ad libitum HFD decreases mouse home cage activity over time. A, Time course of mouse weight over time. B, Point plot showing average weights at weeks 0 and 9; HFD group had significant increase in weight compared to chow; p = 3.475723e-09, F = 60.093562. C, Time course showing, over nine weeks, mice fed HFD exhibited decreasing activity over time relative to chow controls. D, Point plot showing average activity levels normalized to baseline between chow and HFD groups; HFD group had significantly decreased average activity levels compared to chow; p = 0.000997, F = 13.300153. In B, D, two-way ANOVAs performed, stats reported for significant interactions between group and time; n = 10 mice per group.
Tables
Hardware name RAD Subject area Behavioral neuroscience Hardware type In-lab sensor Open source license GNU General Public License v3.0 Cost of hardware $85 Source file repository https://hackaday.io/project/160742homecage-activity-monitoring-with-pirs Component Cost per unit Source of materials PIR sensor $14.53 Olimex, product #MSP430-PIR Jumper wires $1.95 Adafruit, product #1956 M0 Adalogger $19.95 Adafruit, product #2796 FeatherWing oLED $14.95 Adafruit, product #2900 Battery $29.50 Adafruit, product #353 SD card $5.39 NewEgg, product #9SIAC3J63S1330 3D printed housing – – Total $86.27 All components needed to build one RAD device.
MM:DD:YYYY hh:mm:ss Elapsed time Device PIRCount PIRDuration BatteryVoltage 10/25/2018 11:10 0:01:00 39 15 21.51 4.18 10/25/2018 11:11 0:02:00 39 19 26 4.17 10/25/2018 11:12 0:03:00 39 27 32.43 4.15 10/25/2018 11:13 0:04:00 39 40 46.9 4.16 10/25/2018 11:14 0:05:00 39 49 53.4 4.16 10/25/2018 11:15 0:06:00 39 55 59.18 4.16 10/25/2018 11:16 0:07:00 39 68 70.78 4.16 10/25/2018 11:17 0:08:00 39 77 78.87 4.17
Extended Data 1
Data contain the following files: “AdaLoggerM0-SetClock” contains code for setting the real time clock on the Adalogger M0 board; “RAD_activity_counter” contains the main Arduino code to run RAD; “PIR monitor housing” is a 3D file of the housing; “RAD Sample Data” contains sample data from four devices over multiple days; “RAD_analysis” contains python scripts for data anlaysis; “RAD_libraries_031219” contains the Arduino libraries required for RAD. Download Extended Data 1, ZIP file.
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