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Research ArticleMethods/New Tools, Novel Tools and Methods

Critical Analysis of Particle Detection Artifacts in Synaptosome Flow Cytometry

Benjamin D. Hobson and Peter A. Sims
eNeuro 22 May 2019, 6 (3) ENEURO.0009-19.2019; https://doi.org/10.1523/ENEURO.0009-19.2019
Benjamin D. Hobson
1Department of Systems Biology, Columbia University Irving Medical Center, New York, NY 10032
2Department of Biochemistry and Molecular Biophysics, Columbia University Irving Medical Center, New York, NY 10032
3Medical Scientist Training Program, Columbia University Irving Medical Center, New York, NY 10032
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Peter A. Sims
1Department of Systems Biology, Columbia University Irving Medical Center, New York, NY 10032
2Department of Biochemistry and Molecular Biophysics, Columbia University Irving Medical Center, New York, NY 10032
4Sulzberger Columbia Genome Center, Columbia University Irving Medical Center, New York, NY 10032
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Article Information

DOI 
https://doi.org/10.1523/ENEURO.0009-19.2019
PubMed 
31118205
Published By 
Society for Neuroscience
History 
  • Received January 7, 2019
  • Revision received April 1, 2019
  • Accepted April 27, 2019
  • Published online May 22, 2019.
Copyright & Usage 
Copyright © 2019 Hobson and Sims This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Benjamin D. Hobson1,2,3 and
  2. Peter A. Sims1,2,4
  1. 1Department of Systems Biology, Columbia University Irving Medical Center, New York, NY 10032
  2. 2Department of Biochemistry and Molecular Biophysics, Columbia University Irving Medical Center, New York, NY 10032
  3. 3Medical Scientist Training Program, Columbia University Irving Medical Center, New York, NY 10032
  4. 4Sulzberger Columbia Genome Center, Columbia University Irving Medical Center, New York, NY 10032
  1. Correspondence should be addressed to Benjamin D. Hobson at bh2573{at}columbia.edu or Peter A. Sims at pas2182{at}columbia.edu
View Full Text

Author contributions

  1. Author contributions: B.D.H. and P.A.S. designed research; B.D.H. performed research; B.D.H. analyzed data; B.D.H. and P.A.S. wrote the paper.

View Abstract

Disclosures

  • The authors declare no competing financial interests.

  • This work was supported by the National Institute of General Medical Sciences Grant T32GM007367 (to B.D.H.), the National Institute on Drug Abuse Grant 1F30DA047775-01 (to B.D.H.), and the National Institute of Neurological Disorders and Stroke Grant R01NS103473 (to P.A.S.). Research reported in this publication was performed in the CCTI Flow Cytometry Core, supported in part by the Office of the Director, National Institutes of Health under Awards S10OD020056. These studies used the resources of the Cancer Center Flow Core Facility funded in part through Center Grant P30CA013696.

Funding

  • HHS | NIH | National Institute of General Medical Sciences (NIGMS)

    T32GM007367
  • HHS | NIH | National Institute of Neurological Disorders and Stroke (NINDS)

    R01NS103473
  • HHS | NIH | National Institute on Drug Abuse (NIDA)

    F30DA047775

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  • You are viewing the most recent version of this article.
  • previous version (May 22, 2019).

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May 2019294050
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May 202611413
Total 202669813222
Total112978602565
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Critical Analysis of Particle Detection Artifacts in Synaptosome Flow Cytometry
Benjamin D. Hobson, Peter A. Sims
eNeuro 22 May 2019, 6 (3) ENEURO.0009-19.2019; DOI: 10.1523/ENEURO.0009-19.2019

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Critical Analysis of Particle Detection Artifacts in Synaptosome Flow Cytometry
Benjamin D. Hobson, Peter A. Sims
eNeuro 22 May 2019, 6 (3) ENEURO.0009-19.2019; DOI: 10.1523/ENEURO.0009-19.2019
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Keywords

  • flow cytometry
  • synaptosomes

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