Fig. 1A     
Ethanol consumption     
WT and SA/SA mice (5%)  Normal distribution  Welch t test unpaired  WT: n = 6, SA/SA: n = 7  CI(d): –2.681 and –0.273, t = –2.867, p = 0.0226 
WT and SA/SA mice (10%)  Normal distribution  Welch t test unpaired  WT: n = 5, SA/SA: n = 5  CI(d): –1.730 and –0.258, t = –3.562, p = 0.0184 
WT and SD/SD mice (5%)  Normal distribution  Welch t test unpaired  WT: n = 7, SD/SD: n = 6  CI(d): –1.705 and 0.765, t = –0.906, p = 0.3960 
WT and SD/SD mice (10%)  Nonnormal distribution  Mann–Whitney U test  WT: n = 8, SD/SD: n = 8  U = 26, p = 0.5286 
Fig. 1B     
Ethanol preference ratio     
WT and SA/SA mice (5%)  Normal distribution  Welch t test unpaired  WT: n = 6, SA/SA: n = 7  CI(d): –0.444 and –0.056, t = –2.987, p = 0.0179 
WT and SA/SA mice (10%)  Normal distribution  Welch t test unpaired  WT: n = 5, SA/SA: n = 5  CI(d): –0.177 and –0.039, t = –3.724, p = 0.0080 
WT and SD/SD mice (5%)  Normal distribution  Welch t test unpaired  WT: n = 7, SD/SD: n = 6  CI(d): –0.311 and 0.187, t = –0.588, p = 0.5747 
WT and SD/SD mice (10%)  Normal distribution  Welch t test unpaired  WT: n = 8, SD/SD: n = 8  CI(d): –0.109 and 0.094, t = –0.155, p = 0.8791 
Fig. 1C     
Total liquid intake     
WT and SA/SA mice (5%)  Normal distribution  Welch t test unpaired  WT: n = 6, SA/SA: n = 7  CI(d): –0.130 and 0.296, t = 0.916, p = 0.3900 
WT and SA/SA mice (10%)  Normal distribution  Welch t test unpaired  WT: n = 5, SA/SA: n = 5  CI(d): –0.156 and 0.133, t = –0.190, p = 0.8546 
WT and SD/SD mice (5%)  Normal distribution  Welch t test unpaired  WT: n = 7, SD/SD: n = 6  CI(d): –0.272 and 0.117, t = –0.877, p = 0.3999 
WT and SD/SD mice (10%)  Normal distribution  Welch t test unpaired  WT: n = 8, SD/SD: n = 8  CI(d): –0.223 and 0.191, t = –0.166, p = 0.8712 
Fig. 2A     
Taste preference ratio (WT and SA/SA)     
Sucrose  Normal distribution  Paired factorial ANOVA  WT: n = 5, SA/SA: n = 5  Genotype: F = 0.5107, p = 0.4951 
0.033% (w/v)   Bonferroni’s post hoc   CI(m): WT, 0.477 and 0.569; SA/SA, 0.489 and 0.580, p = 0.5901 
0.1% (w/v)   Bonferroni’s post hoc   CI(m): WT, 0.430 and 0.521; SA/SA, 0.447 and 0.538, p = 0.4224 
Quinine  Normal distribution  Paired factorial ANOVA  WT: n = 5, SA/SA: n = 5  Genotype: F = 0.0331, p = 0.8602 
0.02 mM   Bonferroni’s post hoc   CI(m): WT, 0.134 and 0.522; SA/SA, 0.139 and 0.527, p = 0.9484 
0.04 mM   Bonferroni’s post hoc   CI(m): WT, 0.033 and 0.421; SA/SA, 0.060 and 0.448, p = 0.6783 
Fig. 2B     
Taste preference ratio (WT and SD/SD)     
Sucrose  Normal distribution  Paired factorial ANOVA  WT: n = 5, SD/SD: n = 5  Genotype: F = 0.8475, p = 0.3842 
0.033% (w/v)   Bonferroni’s post hoc   CI(m): WT, 0.495 and 0.604; SD/SD, 0.447 and 0.557, p = 0.0533 
0.1% (w/v)   Bonferroni’s post hoc   CI(m): WT, 0.518 and 0.627; SD/SD, 0.522 and 0.631, p = 0.8722 
Quinine  Normal distribution  Paired factorial ANOVA  WT: n = 5, SD/SD: n = 5  Genotype: F = 0.4920, p = 0.5029 
0.02 mM   Bonferroni’s post hoc   CI(m): WT, 0.231 and 0.540; SD/SD, 0.190 and 0.499, p = 0.4454 
0.04 mM   Bonferroni’s post hoc   CI(m): WT, 0.056 and 0.365; SD/SD, 0.003 and 0.312, p = 0.3294 
Fig. 2C     
Total sucrose intake     
WT and SA/SA mice  Normal distribution  Paired factorial ANOVA  WT: n = 5, SA/SA: n = 5  Genotype: F = 0.3578, p = 0.5663 
0.033% (w/v)   Bonferroni’s post hoc   CI(m): WT, 0.442 and 1.558; SA/SA, 0.578 and 1.694, p = 0.4868 
0.1% (w/v)   Bonferroni’s post hoc   CI(m): WT, 2.105 and 3.221; SA/SA, 2.258 and 3.375, p = 0.4345 
WT and SD/SD mice  Normal distribution  Paired factorial ANOVA  WT: n = 5, SD/SD: n = 5  Genotype: F = 0.2553, p = 0.6270 
0.033% (w/v)   Bonferroni’s post hoc   CI(m): WT, 0.442 and 1.558; SD/SD, 0.214 and 1.329, p = 0.2855 
0.1% (w/v)   Bonferroni’s post hoc   CI(m): WT, 2.359 and 3.475; SD/SD, 2.343 and 3.458, p = 0.9385 
Fig. 3     
Blood ethanol concentration     
WT and SA/SA mice  Normal distribution  Paired factorial ANOVA  WT: n = 6, SA/SA: n = 5  Genotype: F = 0.7817, p = 0.3996 
1 h   Tukey–Kramer post hoc   CI(m): WT, 2085.307 and 2908.035; SA/SA, 2108.846 and 3010.099, p = 0.6879 
3 h   Tukey–Kramer post hoc   CI(m): WT, 1063.610 and 1886.337; SA/SA, 1298.783 and 2200.036, p = 0.0947 
WT and SD/SD mice  Normal distribution  Paired factorial ANOVA  WT: n = 5, SD/SD: n = 4  Genotype: F = 0.1381, p = 0.7212 
1 h   Tukey–Kramer post hoc   CI(m): WT, 2115.538 and 3687.719; SD/SD, 2109.947 and 3867.699, p = 0.7418 
3 h   Tukey–Kramer post hoc   CI(m): WT, 1211.065 and 2783.246; SD/SD, 769.016 and 2526.767, p = 0.2089 
Fig. 4     
LORR     
WT and SA/SA mice  Normal distribution  Welch t test unpaired  WT: n = 13, SA/SA: n = 13  CI(d): –12.835 and 11.450, t = –0.118, p = 0.9072 
WT and SD/SD mice  Normal distribution  Welch t test unpaired  WT: n = 16, SD/SD: n = 13  CI(d): –6.790 and 28.367, t = 1.261, p = 0.2184 
Fig. 5A     
ROCK activity     
WT and SA/SA mice  Assuming normality  Welch t test unpaired  WT: n = 4, SA/SA: n = 4  CI(d): 18.691 and 158.488, t = 3.305, p = 0.0228 
WT and SD/SD mice  Assuming normality  Welch t test unpaired  WT: n = 4, SD/SD: n = 4  CI(d): –157.109 and 115.935, t = –0.421, p = 0.6956 
Fig. 5B     
ROCK protein level     
WT and SA/SA mice  Assuming normality  Welch t test unpaired  WT: n = 4, SA/SA: n = 4  CI(d): –0.015 and 0.014, t = –0.141, p = 0.8929 
WT and SD/SD mice  Assuming normality  Welch t test unpaired  WT: n = 4, SD/SD: n = 4  CI(d): –0.032 and 0.085, t = 1.248, p = 0.2782 
Fig. 5C     
ROCK activity/protein.     
WT and SA/SA mice  Assuming normality  Welch t test unpaired  WT: n = 4, SA/SA: n = 4  CI(d): 194.255 and 925.975, t = 3.747, p = 0.0096 
WT and SD/SD mice  Assuming normality  Welch t test unpaired  WT: n = 4, SD/SD: n = 4  CI(d): –1286.927 and 604.178, t = –0.937, p = 0.3929 

Fig. 5D     
Phosphorylated Akt     
WT and SA/SA mice  Normal distribution  Welch t test unpaired  WT: n = 5, SA/SA: n = 5  CI(d): –0.077 and –0.007, t = –2.751, p = 0.0256 
Table 2     
Body weight     
Fig. 1 (ethanol twobottle choice test)     
WT and SA/SA mice (5%)  Normal distribution  Welch t test unpaired  WT: n = 6, SA/SA: n = 7  CI(d): –0.003 and 0.002, t = –0.512, p = 0.6229 
WT and SA/SA mice (10%)  Normal distribution  Welch t test unpaired  WT: n = 5, SA/SA: n = 5  CI(d): –0.002 and 0.009, t = 1.561, p = 0.1651 
WT and SD/SD mice (5%)  Normal distribution  Welch t test unpaired  WT: n = 7, SD/SD: n = 6  CI(d): –0.001 and 0.007, t = 1.907, p = 0.0874 
WT and SD/SD mice (10%)  Normal distribution  Welch t test unpaired  WT: n = 8, SD/SD: n = 8  CI(d): –0.003 and 0.003, t = 0.029, p = 0.9770 
Fig. 2 (taste preference test)     
WT/WT and SA/SA mice  Normal distribution  Welch t test unpaired  WT: n = 5, SA/SA: n = 5  CI(d): –0.005 and 0.007, t = 0.435, p = 0.6769 
WT/WT and SD/SD mice  Normal distribution  Welch t test unpaired  WT: n = 5, SA/SA: n = 5  CI(d): –0.004 and 0.009, t = 0.847, p = 0.4226 
Table 3     
Putative average ethanol consumption     
WT and SA/SA mice     
5% ethanol  Normal distribution  Welch t test unpaired  WT: n = 6, SA/SA: n = 7  CI(d): –2.223 and –0.209, t = –2.809, p = 0.0240 
10% ethanol  Normal distribution  Welch t test unpaired  WT: n = 5, SA/SA: n = 5  CI(d): –1.707 and –0.305, t = –3.309, p = 0.0107 
WT and SD/SD mice     
5% ethanol  Normal distribution  Welch t test unpaired  WT: n = 7, SD/SD: n = 6  CI(d): –1.692 and 0.833, t = –0.820, p = 0.4414 
10% ethanol  Nonnormal distribution  Mann–Whitney U test  WT: n = 8, SD/SD: n = 8  U = 26, p = 0.5286 
Table 4     
Putative average total liquid intake     
WT and SA/SA mice     
5% ethanol  Normal distribution  Welch t test unpaired  WT: n = 6, SA/SA: n = 7  CI(d): –19.2958 and 42.1470, t = 0.8822, p = 0.4074 
10% ethanol  Normal distribution  Welch t test unpaired  WT: n = 5, SA/SA: n = 5  CI(d): –12.7726 and 19.1717, t = 0.4800, p = 0.6468 
WT and SD/SD mice     
5% ethanol  Normal distribution  Welch t test unpaired  WT: n = 7, SD/SD: n = 6  CI(d): –29.0687 and 14.5159, t = –0.7377, p = 0.4766 
10% ethanol  Normal distribution  Welch t test unpaired  WT: n = 8, SD/SD: n = 8  CI(d): –25.3723 and 22.8610, t = –0.1142, p = 0.9111 