Skip to main content

Main menu

  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Current Issue
    • Issue Archive
    • Blog
    • Collections
    • Podcast
  • TOPICS
    • Cognition and Behavior
    • Development
    • Disorders of the Nervous System
    • History, Teaching and Public Awareness
    • Integrative Systems
    • Neuronal Excitability
    • Novel Tools and Methods
    • Sensory and Motor Systems
  • ALERTS
  • FOR AUTHORS
  • ABOUT
    • Overview
    • Editorial Board
    • For the Media
    • Privacy Policy
    • Contact Us
    • Feedback
  • SUBMIT

User menu

Search

  • Advanced search
eNeuro
eNeuro

Advanced Search

 

  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Current Issue
    • Issue Archive
    • Blog
    • Collections
    • Podcast
  • TOPICS
    • Cognition and Behavior
    • Development
    • Disorders of the Nervous System
    • History, Teaching and Public Awareness
    • Integrative Systems
    • Neuronal Excitability
    • Novel Tools and Methods
    • Sensory and Motor Systems
  • ALERTS
  • FOR AUTHORS
  • ABOUT
    • Overview
    • Editorial Board
    • For the Media
    • Privacy Policy
    • Contact Us
    • Feedback
  • SUBMIT
PreviousNext
Research ArticleNew Research, Cognition and Behavior

Aerobic Glycolysis Is Required for Spatial Memory Acquisition But Not Memory Retrieval in Mice

Richard A. Harris, Asad Lone, Heeseung Lim, Francisco Martinez, Ariel K. Frame, Timothy J. Scholl and Robert C. Cumming
eNeuro 11 February 2019, 6 (1) ENEURO.0389-18.2019; https://doi.org/10.1523/ENEURO.0389-18.2019
Richard A. Harris
1Department of Biology, University of Western Ontario, London, Ontario N6A 5B7, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Asad Lone
1Department of Biology, University of Western Ontario, London, Ontario N6A 5B7, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Heeseung Lim
2Department of Medical Biophysics, University of Western Ontario, London, Ontario N6A 5C1, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Francisco Martinez
3Robarts Research Institute, University of Western Ontario, London, Ontario N6A 3K7, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ariel K. Frame
1Department of Biology, University of Western Ontario, London, Ontario N6A 5B7, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Ariel K. Frame
Timothy J. Scholl
2Department of Medical Biophysics, University of Western Ontario, London, Ontario N6A 5C1, Canada
3Robarts Research Institute, University of Western Ontario, London, Ontario N6A 3K7, Canada
4 Ontario Institute for Cancer Research, Toronto, Ontario M5G 0A3, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Timothy J. Scholl
Robert C. Cumming
1Department of Biology, University of Western Ontario, London, Ontario N6A 5B7, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Robert C. Cumming
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Article Figures & Data

Figures

  • Figure 1.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 1.

    Hyperpolarized 13C-pyruvate magnetic resonance spectroscopic imaging reveals a decline in lactate following DCA administration. A, Injection and imaging regime of 9-month-old mice using hyperpolarized MRSI of [1-13C] pyruvate to measure the conversion of pyruvate to lactate. Mice were imaged after the first 13C-pyruvate injection (Before) and 30 min later were then injected with DCA (200 mg/kg). Following a 30 min recovery time, another injection of 13C-pyruvate and imaging were performed (After). B, 1H MRI of the brain in the coronal field overlayed with MRSI voxels containing spectra of 13C-labeled pyruvate and lactate (yellow). C, Conversion of pyruvate to lactate was measured as a ratio of the observed lactate peak to pyruvate peak from before DCA injection (blue line) and after (red dashed line). A pyruvate hydrate peak was also recorded. D, DCA injection reduces the ratio of lactate to pyruvate in the mouse brain (p = 0.04). Data shown are the mean ± SEM. n = 4.

  • Figure 2.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 2.

    DCA injection reduces the phosphorylation of PDH in the frontal cortex and hippocampus. A, B, Western blot analysis (left) was performed on extracts from the frontal cortex (A) and the hippocampus (B) of mice intraperitoneally injected with either saline or DCA (200 mg/kg) 30 min before being killed. Densitometric analysis of Western blots (right) revealed significantly lower PDH phosphorylation in DCA-treated mice relative to saline-injected mice (*p < 0.05, ***p < 0.001; n = 6 and 6, respectively, for extracts from the frontal cortex of saline- and DCA-injected mice; and n = 7 and 7, respectively, for extracts from the hippocampus of saline- and DCA-injected mice). Band densities were standardized to β-actin controls. Data shown are the mean ± SEM.

  • Figure 3.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 3.

    DCA administration causes impairment in spatial learning. Mice were intraperitoneally injected with saline or DCA (200 mg/kg) 30 min before each day of training and allowed to find the location of a hidden platform in the northwest quadrant. A–C, The latency to find the platform (A), the total path length (B), and the mean speed (C) were recorded on each training day. D–G, On day 5, a probe trial was performed without DCA injection, and mice were allowed to swim for 60 s. D, The swim path for each group of mice was compiled into heat map representations. E–G, Measurements were taken for the total distance traveled (E), the percentage of time spent in the correct quadrant (F), and the number of times the boundary of the platform was crossed (G). H, A week after the first probe trial, mice were again tested with a second probe trial, and measurements were taken for the number of times the boundary of the platform was crossed. Data shown are the mean ± SEM. *p < 0.05, **p < 0.01. n = 7 and 7 for saline and DCA treatments, respectively.

  • Figure 4.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 4.

    DCA administration does not interfere with memory retrieval. MWM performed by mice intraperitoneally injected with saline or DCA (200 mg/kg) on the probe trial. A, Mice were trained for 4 consecutive days (4 trials/d) to find the location of a hidden platform in the southwest quadrant, and the latency to escape was recorded. B–G, On day 5, a probe trial was performed in which the platform was removed and mice were allowed to swim for 60 s. B, The swim path for each group of mice was recorded and compiled into heat map representations. C–E, Measurements were taken for the total distance traveled (C), the percentage of time spent in the correct quadrant (D), and the number of times the boundary of the platform was crossed (E). F, Immediately after the probe trial, a flag trial was performed and the latency to find the flag was recorded. Data shown are the mean ± SEM. n = 9 and 10 for saline and DCA treatments, respectively. No significant differences were observed between saline-treated (sham) and DCA-injected mice.

Back to top

In this issue

eneuro: 6 (1)
eNeuro
Vol. 6, Issue 1
January/February 2019
  • Table of Contents
  • Index by author
  • Ed Board (PDF)
Email

Thank you for sharing this eNeuro article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Aerobic Glycolysis Is Required for Spatial Memory Acquisition But Not Memory Retrieval in Mice
(Your Name) has forwarded a page to you from eNeuro
(Your Name) thought you would be interested in this article in eNeuro.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Print
View Full Page PDF
Citation Tools
Aerobic Glycolysis Is Required for Spatial Memory Acquisition But Not Memory Retrieval in Mice
Richard A. Harris, Asad Lone, Heeseung Lim, Francisco Martinez, Ariel K. Frame, Timothy J. Scholl, Robert C. Cumming
eNeuro 11 February 2019, 6 (1) ENEURO.0389-18.2019; DOI: 10.1523/ENEURO.0389-18.2019

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Respond to this article
Share
Aerobic Glycolysis Is Required for Spatial Memory Acquisition But Not Memory Retrieval in Mice
Richard A. Harris, Asad Lone, Heeseung Lim, Francisco Martinez, Ariel K. Frame, Timothy J. Scholl, Robert C. Cumming
eNeuro 11 February 2019, 6 (1) ENEURO.0389-18.2019; DOI: 10.1523/ENEURO.0389-18.2019
Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Significance Statement
    • Introduction
    • Materials and Methods
    • Results
    • Discussion
    • Footnotes
    • References
    • Synthesis
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Keywords

  • aerobic glycolysis
  • lactate
  • magnetic resonance spectroscopy
  • memory
  • metabolism
  • synaptic plasticity

Responses to this article

Respond to this article

Jump to comment:

No eLetters have been published for this article.

Related Articles

Cited By...

More in this TOC Section

New Research

  • A Very Fast Time Scale of Human Motor Adaptation: Within Movement Adjustments of Internal Representations during Reaching
  • TrkB Signaling Influences Gene Expression in Cortistatin-Expressing Interneurons
  • Optogenetic Activation of β-Endorphin Terminals in the Medial Preoptic Nucleus Regulates Female Sexual Receptivity
Show more New Research

Cognition and Behavior

  • Transformed visual working memory representations in human occipitotemporal and posterior parietal cortices
  • Neural Speech-Tracking During Selective Attention: A Spatially Realistic Audiovisual Study
  • Nucleus Accumbens Dopamine Encodes the Trace Period during Appetitive Pavlovian Conditioning
Show more Cognition and Behavior

Subjects

  • Cognition and Behavior
  • Home
  • Alerts
  • Follow SFN on BlueSky
  • Visit Society for Neuroscience on Facebook
  • Follow Society for Neuroscience on Twitter
  • Follow Society for Neuroscience on LinkedIn
  • Visit Society for Neuroscience on Youtube
  • Follow our RSS feeds

Content

  • Early Release
  • Current Issue
  • Latest Articles
  • Issue Archive
  • Blog
  • Browse by Topic

Information

  • For Authors
  • For the Media

About

  • About the Journal
  • Editorial Board
  • Privacy Notice
  • Contact
  • Feedback
(eNeuro logo)
(SfN logo)

Copyright © 2025 by the Society for Neuroscience.
eNeuro eISSN: 2373-2822

The ideas and opinions expressed in eNeuro do not necessarily reflect those of SfN or the eNeuro Editorial Board. Publication of an advertisement or other product mention in eNeuro should not be construed as an endorsement of the manufacturer’s claims. SfN does not assume any responsibility for any injury and/or damage to persons or property arising from or related to any use of any material contained in eNeuro.