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Research ArticleMethods/New Tools, Novel Tools and Methods

Embryonic Neocortical Microglia Express Toll-Like Receptor 9 and Respond to Plasmid DNA Injected into the Ventricle: Technical Considerations Regarding Microglial Distribution in Electroporated Brain Walls

Yuki Hattori and Takaki Miyata
eNeuro 16 November 2018, 5 (6) ENEURO.0312-18.2018; https://doi.org/10.1523/ENEURO.0312-18.2018
Yuki Hattori
Department of Anatomy and Cell Biology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan
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Takaki Miyata
Department of Anatomy and Cell Biology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan
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Article Information

DOI 
https://doi.org/10.1523/ENEURO.0312-18.2018
PubMed 
30627652
Published By 
Society for Neuroscience
History 
  • Received August 9, 2018
  • Revision received October 19, 2018
  • Accepted October 27, 2018
  • Published online November 16, 2018.
Copyright & Usage 
Copyright © 2018 Hattori and Miyata This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Yuki Hattori and
  2. Takaki Miyata
  1. Department of Anatomy and Cell Biology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan
  1. Yuki Hattori, Department of Anatomy and Cell Biology, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa, Nagoya, Aichi, 466-8550, Japan. E-mail: ha-yuki{at}med.nagoya-u.ac.jp.
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Author contributions

  1. Author Contributions: Y.H. and T.M. designed research and wrote the paper; Y.H. performed the experiments and analyzed the data; Y.H. and T.M. approved the final version of the paper.

Disclosures

  • The authors declare no competing financial interests.

  • This work was supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI Grants 16H02457 and 16K15169 (to T.M.), 16J06207 (Grant-in-Aid for JSPS Fellows; to Y.H.), and 18K15003 (Grant-in-Aid for Young Scientists; to Y.H.; Y.H. is a Research Fellow of JSPS). We thank Makoto Masaoka and Namiko Noguchi (Department of Anatomy and Cell Biology, Nagoya University Graduate School of Medicine) for technical assistance.

Funding

  • Japan Society for the Promotion of Science (JSPS)

    16J06207; 18K15003; 16H02457; 16K15169

Other Version

  • You are viewing the most recent version of this article.
  • previous version (November 16, 2018).

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Total 202561818176
Total159285431404
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eneuro: 5 (6)
eNeuro
Vol. 5, Issue 6
November/December 2018
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Embryonic Neocortical Microglia Express Toll-Like Receptor 9 and Respond to Plasmid DNA Injected into the Ventricle: Technical Considerations Regarding Microglial Distribution in Electroporated Brain Walls
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Embryonic Neocortical Microglia Express Toll-Like Receptor 9 and Respond to Plasmid DNA Injected into the Ventricle: Technical Considerations Regarding Microglial Distribution in Electroporated Brain Walls
Yuki Hattori, Takaki Miyata
eNeuro 16 November 2018, 5 (6) ENEURO.0312-18.2018; DOI: 10.1523/ENEURO.0312-18.2018

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Embryonic Neocortical Microglia Express Toll-Like Receptor 9 and Respond to Plasmid DNA Injected into the Ventricle: Technical Considerations Regarding Microglial Distribution in Electroporated Brain Walls
Yuki Hattori, Takaki Miyata
eNeuro 16 November 2018, 5 (6) ENEURO.0312-18.2018; DOI: 10.1523/ENEURO.0312-18.2018
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Keywords

  • in utero electroporation
  • live-imaging
  • microglia
  • TLR4
  • TLR9
  • toll-like receptor

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