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Research ArticleMethods/New Tools, Novel Tools and Methods

High-Fidelity Imaging in Brain-Wide Structural Studies Using Light-Sheet Microscopy

M. Caroline Müllenbroich, Ludovico Silvestri, Antonino P. Di Giovanna, Giacomo Mazzamuto, Irene Costantini, Leonardo Sacconi and Francesco S. Pavone
eNeuro 14 November 2018, 5 (6) ENEURO.0124-18.2018; https://doi.org/10.1523/ENEURO.0124-18.2018
M. Caroline Müllenbroich
1National Institute of Optics, National Research Council, Sesto Fiorentino, 50019, Italy
2European Laboratory for Non-Linear Spectroscopy, LENS, Sesto Fiorentino, 50019, Italy
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  • ORCID record for M. Caroline Müllenbroich
Ludovico Silvestri
1National Institute of Optics, National Research Council, Sesto Fiorentino, 50019, Italy
2European Laboratory for Non-Linear Spectroscopy, LENS, Sesto Fiorentino, 50019, Italy
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Antonino P. Di Giovanna
2European Laboratory for Non-Linear Spectroscopy, LENS, Sesto Fiorentino, 50019, Italy
3University of Florence, Italy
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Giacomo Mazzamuto
2European Laboratory for Non-Linear Spectroscopy, LENS, Sesto Fiorentino, 50019, Italy
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Irene Costantini
2European Laboratory for Non-Linear Spectroscopy, LENS, Sesto Fiorentino, 50019, Italy
3University of Florence, Italy
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Leonardo Sacconi
1National Institute of Optics, National Research Council, Sesto Fiorentino, 50019, Italy
2European Laboratory for Non-Linear Spectroscopy, LENS, Sesto Fiorentino, 50019, Italy
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Francesco S. Pavone
1National Institute of Optics, National Research Council, Sesto Fiorentino, 50019, Italy
2European Laboratory for Non-Linear Spectroscopy, LENS, Sesto Fiorentino, 50019, Italy
4Department of Physics and Astronomy, University of Florence, Sesto Fiorentino 50019, Italy
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Article Information

DOI 
https://doi.org/10.1523/ENEURO.0124-18.2018
Published By 
Society for Neuroscience
History 
  • Received March 29, 2018
  • Revision received July 26, 2018
  • Accepted August 22, 2018
  • Published online November 14, 2018.
Copyright & Usage 
Copyright © 2018 Müllenbroich et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. M. Caroline Müllenbroich1,2,
  2. Ludovico Silvestri1,2,
  3. Antonino P. Di Giovanna2,3,
  4. Giacomo Mazzamuto2,
  5. Irene Costantini2,3,
  6. Leonardo Sacconi1,2 and
  7. Francesco S. Pavone1,2,4
  1. 1National Institute of Optics, National Research Council, Sesto Fiorentino, 50019, Italy
  2. 2European Laboratory for Non-Linear Spectroscopy, LENS, Sesto Fiorentino, 50019, Italy
  3. 3University of Florence, Italy
  4. 4Department of Physics and Astronomy, University of Florence, Sesto Fiorentino 50019, Italy
  1. Correspondence should be addressed to M. Caroline Müllenbroich at the above address, E-mail: muellenbroich{at}lens.unifi.it.
  • Müllenbroich’s present address: School of Physics, Kelvin Building, University of Glasgow, G12 8QQ Glasgow, Scotland, United Kingdom.

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Author contributions

  1. Author contributions: M.C.M., L.Si., and L.Sa. designed research; A.P.D.G., I.C., M.C.M., L.Si., G.M. performed research; M.C.M analyzed data; M.C.M wrote the paper; F.S.P. acquired all funding and supervised the project.

  • Müllenbroich’s present address: School of Physics, Kelvin Building, University of Glasgow, G12 8QQ Glasgow, Scotland, United Kingdom.

Disclosures

  • The authors declare no competing financial interests.

  • This project was supported by European Union’s H2020 Research and Innovation Programme Grants 720270 (Human Brain Project) and 654148 (Laserlab-Europe) and by the European Union’s Programme H2020 EXCELLENT SCIENCE - European Research Council (ERC) Grant 692943 (BrainBIT). The project has also been supported by the Italian Ministry for Education, University, and Research in the framework of the Flagship Project NanoMAX and of Eurobioimaging Italian Nodes (ESFRI research infrastructure), and by “Ente Cassa di Risparmio di Firenze” (private foundation).

Funding

  • Human Brain Project

    720270
  • Laserlab Europe

    654148
  • EC | European Research Council (ERC)

    692943
  • NanoMAX

  • Ente Cassa di Risparmio di Firenze (Cash Savings Institution of Florence)

  • EurobioImaging

Other Version

  • You are viewing the most recent version of this article.
  • previous version (November 14, 2018).

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Total142563131935
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eneuro: 5 (6)
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November/December 2018
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High-Fidelity Imaging in Brain-Wide Structural Studies Using Light-Sheet Microscopy
M. Caroline Müllenbroich, Ludovico Silvestri, Antonino P. Di Giovanna, Giacomo Mazzamuto, Irene Costantini, Leonardo Sacconi, Francesco S. Pavone
eNeuro 14 November 2018, 5 (6) ENEURO.0124-18.2018; DOI: 10.1523/ENEURO.0124-18.2018

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High-Fidelity Imaging in Brain-Wide Structural Studies Using Light-Sheet Microscopy
M. Caroline Müllenbroich, Ludovico Silvestri, Antonino P. Di Giovanna, Giacomo Mazzamuto, Irene Costantini, Leonardo Sacconi, Francesco S. Pavone
eNeuro 14 November 2018, 5 (6) ENEURO.0124-18.2018; DOI: 10.1523/ENEURO.0124-18.2018
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Keywords

  • light-sheet microscopy
  • structural imaging
  • whole-brain imaging
  • mouse
  • vascular/neuronal networks

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