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Research ArticleNew Research, Disorders of the Nervous System

In Vivo Volume Dynamics of Dendritic Spines in the Neocortex of Wild-Type and Fmr1 KO Mice

Kazuhiko Ishii, Akira Nagaoka, Yutaro Kishida, Hitoshi Okazaki, Sho Yagishita, Hasan Ucar, Noriko Takahashi, Nobuhito Saito and Haruo Kasai
eNeuro 19 October 2018, 5 (5) ENEURO.0282-18.2018; https://doi.org/10.1523/ENEURO.0282-18.2018
Kazuhiko Ishii
1Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan
2Department of Neurosurgery, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku Tokyo 113-0033, Japan
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Akira Nagaoka
1Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan
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Yutaro Kishida
1Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan
3International Research Center for Neurointelligence, The University of Tokyo Institutes for Advanced Study, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan
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Hitoshi Okazaki
1Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan
3International Research Center for Neurointelligence, The University of Tokyo Institutes for Advanced Study, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan
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Sho Yagishita
1Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan
3International Research Center for Neurointelligence, The University of Tokyo Institutes for Advanced Study, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan
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Hasan Ucar
1Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan
3International Research Center for Neurointelligence, The University of Tokyo Institutes for Advanced Study, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan
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Noriko Takahashi
4Department of Physiology, Kitasato University School of Medicine, Sagamihara 252-0374, Japan
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Nobuhito Saito
2Department of Neurosurgery, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku Tokyo 113-0033, Japan
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Haruo Kasai
1Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan
3International Research Center for Neurointelligence, The University of Tokyo Institutes for Advanced Study, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan
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Article Information

DOI 
https://doi.org/10.1523/ENEURO.0282-18.2018
PubMed 
30417082
Published By 
Society for Neuroscience
History 
  • Received July 12, 2018
  • Revision received September 30, 2018
  • Accepted October 3, 2018
  • Published online October 19, 2018.
Copyright & Usage 
Copyright © 2018 Ishii et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Kazuhiko Ishii1,2,
  2. Akira Nagaoka1,
  3. Yutaro Kishida1,3,
  4. Hitoshi Okazaki1,3,
  5. Sho Yagishita1,3,
  6. Hasan Ucar1,3,
  7. Noriko Takahashi4,
  8. Nobuhito Saito2 and
  9. Haruo Kasai1,3
  1. 1Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan
  2. 2Department of Neurosurgery, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku Tokyo 113-0033, Japan
  3. 3International Research Center for Neurointelligence, The University of Tokyo Institutes for Advanced Study, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan
  4. 4Department of Physiology, Kitasato University School of Medicine, Sagamihara 252-0374, Japan
  1. Correspondence should be addressed to Haruo Kasai, Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, University of Tokyo, Bunkyo-ku, Tokyo, Japan. E-mail: hkasai{at}m.u-Tokyo.ac.jp.
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Author contributions

  1. Author contributions: K.I., A.N., and Y.K. performed research; K.I., H.O., S.Y., H.U., N.T., N.S., and H.K. analyzed data; K.I. and H.K. wrote the paper.

Disclosures

  • The authors declare no competing financial interests.

  • This work was supported by Grants-in-Aid for Scientific Research (S; Grant 26221001 to H.K.), a Young Scientists (B; Grant 15K18333 to S.Y.), and Scientific Research on Innovative Areas (Grant 16H06396 to S.Y.) from The Japan Society for the Promotion of Science; Japan-EU Joint Workshop on Advanced Quantum Technology for Future Innovation (Grant JPMJCR1652 to H.K.) from the Japan Science and Technology Agency; Strategic Research Program for Brain Sciences Projects (17dm0107120h0002) from The Japan Agency for Medical Research and Development (to H.K.); and World Premier International Research Center Initiative from Ministry of Education, Culture, Sports, Science and Technology (to H.K.).

Funding

  • JSPS

    26221001; 15K18333; 16H06396; WPI^IRCN
  • JST

    JPMJCR1652
  • AMED

    17dm0107120h0002

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  • previous version (October 19, 2018).

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Oct 20182450113
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Total 2025102996223
Jan 20265484
Total 20265484
Total110780151534
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In Vivo Volume Dynamics of Dendritic Spines in the Neocortex of Wild-Type and Fmr1 KO Mice
Kazuhiko Ishii, Akira Nagaoka, Yutaro Kishida, Hitoshi Okazaki, Sho Yagishita, Hasan Ucar, Noriko Takahashi, Nobuhito Saito, Haruo Kasai
eNeuro 19 October 2018, 5 (5) ENEURO.0282-18.2018; DOI: 10.1523/ENEURO.0282-18.2018

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In Vivo Volume Dynamics of Dendritic Spines in the Neocortex of Wild-Type and Fmr1 KO Mice
Kazuhiko Ishii, Akira Nagaoka, Yutaro Kishida, Hitoshi Okazaki, Sho Yagishita, Hasan Ucar, Noriko Takahashi, Nobuhito Saito, Haruo Kasai
eNeuro 19 October 2018, 5 (5) ENEURO.0282-18.2018; DOI: 10.1523/ENEURO.0282-18.2018
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Keywords

  • ASD
  • autism
  • dendritic spine
  • fragile X mental retardation
  • synaptic plasticity
  • two-photon microscopy

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