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Research ArticleMethods/New Tools, Novel Tools and Methods

Immediate-Early Promoter-Driven Transgenic Reporter System for Neuroethological Research in a Hemimetabolous Insect

Takayuki Watanabe, Atsushi Ugajin and Hitoshi Aonuma
eNeuro 7 August 2018, 5 (4) ENEURO.0061-18.2018; https://doi.org/10.1523/ENEURO.0061-18.2018
Takayuki Watanabe
1Department of Biological Sciences, Faculty of Science, Hokkaido University, Kita 10 Jo Nishi 6 Chome, Kita-Ku, Sapporo 060-0810, Japan
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  • ORCID record for Takayuki Watanabe
Atsushi Ugajin
2Laboratory of Evolution of Insect Feeding Habit, JT Biohistory Research Hall, Takatsuki 569-1125, Japan
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Hitoshi Aonuma
3Research Center of Mathematics for Social Creativity, Research Institute for Electronic Science, Hokkaido University, Sapporo 060-0811, Japan
4CREST, Japan Science and Technology Agency, Kawaguchi 332-0012, Japan
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Article Information

DOI 
https://doi.org/10.1523/ENEURO.0061-18.2018
PubMed 
30225346
Published By 
Society for Neuroscience
History 
  • Received February 6, 2018
  • Revision received July 11, 2018
  • Accepted July 20, 2018
  • Published online August 7, 2018.
Copyright & Usage 
Copyright © 2018 Watanabe et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Takayuki Watanabe1,
  2. Atsushi Ugajin2 and
  3. Hitoshi Aonuma3,4
  1. 1Department of Biological Sciences, Faculty of Science, Hokkaido University, Kita 10 Jo Nishi 6 Chome, Kita-Ku, Sapporo 060-0810, Japan
  2. 2Laboratory of Evolution of Insect Feeding Habit, JT Biohistory Research Hall, Takatsuki 569-1125, Japan
  3. 3Research Center of Mathematics for Social Creativity, Research Institute for Electronic Science, Hokkaido University, Sapporo 060-0811, Japan
  4. 4CREST, Japan Science and Technology Agency, Kawaguchi 332-0012, Japan
  1. Correspondence should be addressed to Takayuki Watanabe, Department of Biological Sciences, Faculty of Science, Hokkaido University, Kita 10 Jo Nishi 6 Chome, Kita-Ku, Sapporo 060-0810, Japan. E-mail: wtaka226{at}frontier.hokudai.ac.jp.
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Author contributions

  1. Author contributions: T.W. designed the study and wrote the manuscript with the help of A.U. and T.W. performed all experiments and analyzed the data. All the authors reviewed the manuscript.

View Abstract

Disclosures

  • The authors declare no competing financial interests.

  • This work was supported, in part, by the Grant-in-Aid for Japanese Society for the Promotion of Science (JSPS) Research Fellow (24•3065) to TW; the JSPS KAKENHI (16K20875) to TW; and Akiyama Life Science Foundation research grant 2014 to TW. The white-eye mutant of G. bimaculatus (gwhite mutant) was a kind gift of Prof. Sumihare Noji in the University of Tokushima. The pXL-BacII, pBSII-IFP2-orf, and pBSII-ITR1.1k-EYFP plasmids were kind gifts of Prof. Malcolm J. Fraser, Jr. in the University of Notre Dame.

Funding

  • Japan Society for the Promotion of Science (JSPS)

    24•3065; 16K20875
  • Akiyama Life Science Foundation

Other Version

  • You are viewing the most recent version of this article.
  • previous version (August 07, 2018).

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AbstractFullPdf
Aug 2018308049
Sep 20181402625
Oct 201810014532
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Total 2018658292170
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Jun 2025108126
Jul 2025187944
Aug 2025127114
Sep 20252020226
Oct 2025149012
Nov 20252511832
Dec 20252254
Total 2025131992264
Total120063801461
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Immediate-Early Promoter-Driven Transgenic Reporter System for Neuroethological Research in a Hemimetabolous Insect
Takayuki Watanabe, Atsushi Ugajin, Hitoshi Aonuma
eNeuro 7 August 2018, 5 (4) ENEURO.0061-18.2018; DOI: 10.1523/ENEURO.0061-18.2018

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Immediate-Early Promoter-Driven Transgenic Reporter System for Neuroethological Research in a Hemimetabolous Insect
Takayuki Watanabe, Atsushi Ugajin, Hitoshi Aonuma
eNeuro 7 August 2018, 5 (4) ENEURO.0061-18.2018; DOI: 10.1523/ENEURO.0061-18.2018
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Keywords

  • Activity mapping
  • Gryllus bimaculatus
  • immediate-early gene
  • transgenesis

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