Skip to main content

Main menu

  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Current Issue
    • Issue Archive
    • Blog
    • Collections
    • Podcast
  • TOPICS
    • Cognition and Behavior
    • Development
    • Disorders of the Nervous System
    • History, Teaching and Public Awareness
    • Integrative Systems
    • Neuronal Excitability
    • Novel Tools and Methods
    • Sensory and Motor Systems
  • ALERTS
  • FOR AUTHORS
  • ABOUT
    • Overview
    • Editorial Board
    • For the Media
    • Privacy Policy
    • Contact Us
    • Feedback
  • SUBMIT

User menu

Search

  • Advanced search
eNeuro
eNeuro

Advanced Search

 

  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Current Issue
    • Issue Archive
    • Blog
    • Collections
    • Podcast
  • TOPICS
    • Cognition and Behavior
    • Development
    • Disorders of the Nervous System
    • History, Teaching and Public Awareness
    • Integrative Systems
    • Neuronal Excitability
    • Novel Tools and Methods
    • Sensory and Motor Systems
  • ALERTS
  • FOR AUTHORS
  • ABOUT
    • Overview
    • Editorial Board
    • For the Media
    • Privacy Policy
    • Contact Us
    • Feedback
  • SUBMIT
PreviousNext
Research ArticleMethods/New Tools, Novel Tools and Methods

Using Automated Live Cell Imaging to Reveal Early Changes during Human Motor Neuron Degeneration

Hye Young Shin, Kathleen L. Pfaff, Lance S. Davidow, Chicheng Sun, Takayuki Uozumi, Fumiki Yanagawa, Yoichi Yamazaki, Yasujiro Kiyota and Lee L. Rubin
eNeuro 18 June 2018, 5 (3) ENEURO.0001-18.2018; https://doi.org/10.1523/ENEURO.0001-18.2018
Hye Young Shin
1Department of Stem Cell and Regenerative Biology and Harvard Stem Cell Institute, Harvard University, Cambridge, MA, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Hye Young Shin
Kathleen L. Pfaff
1Department of Stem Cell and Regenerative Biology and Harvard Stem Cell Institute, Harvard University, Cambridge, MA, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lance S. Davidow
1Department of Stem Cell and Regenerative Biology and Harvard Stem Cell Institute, Harvard University, Cambridge, MA, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Lance S. Davidow
Chicheng Sun
1Department of Stem Cell and Regenerative Biology and Harvard Stem Cell Institute, Harvard University, Cambridge, MA, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Chicheng Sun
Takayuki Uozumi
2Healthcare Business Unit, Nikon Corporation, Tokyo, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Fumiki Yanagawa
2Healthcare Business Unit, Nikon Corporation, Tokyo, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yoichi Yamazaki
2Healthcare Business Unit, Nikon Corporation, Tokyo, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yasujiro Kiyota
2Healthcare Business Unit, Nikon Corporation, Tokyo, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Yasujiro Kiyota
Lee L. Rubin
1Department of Stem Cell and Regenerative Biology and Harvard Stem Cell Institute, Harvard University, Cambridge, MA, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Lee L. Rubin
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Article Information

DOI 
https://doi.org/10.1523/ENEURO.0001-18.2018
PubMed 
29971247
Published By 
Society for Neuroscience
History 
  • Received December 20, 2017
  • Revision received April 27, 2018
  • Accepted May 12, 2018
  • Published online June 18, 2018.
Copyright & Usage 
Copyright © 2018 Shin et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Hye Young Shin1,
  2. Kathleen L. Pfaff1,
  3. Lance S. Davidow1,
  4. Chicheng Sun1,
  5. Takayuki Uozumi2,
  6. Fumiki Yanagawa2,
  7. Yoichi Yamazaki2,
  8. Yasujiro Kiyota2 and
  9. Lee L. Rubin1
  1. 1Department of Stem Cell and Regenerative Biology and Harvard Stem Cell Institute, Harvard University, Cambridge, MA, United States
  2. 2Healthcare Business Unit, Nikon Corporation, Tokyo, Japan
  1. Address correspondence to Lee Rubin, PhD, Dept of Stem Cell and Regenerative Biology, Harvard University, Director of Translational Medicine, Harvard Stem Cell Institute, Sherman Fairchild G60, 7 Divinity Ave., Cambridge, MA 02138. E-mail: lee_rubin{at}harvard.edu.
View Full Text

Author contributions

  1. Author contributions: H.Y.S., K.L.P., L.S.D., C.S., and L.L.R designed experiments and evaluated results; H.Y.S. performed all experiments; T.U., F.Y., Y.Y., and Y.K. designed and applied live cell morphometric analysis; H.Y.S., K.L.P., and L.L.R. wrote manuscript.

Disclosures

  • The authors declare no competing financial interests.

  • This research was supported by a sponsored research grant from Nikon Corporation to LLR.

Other Version

  • You are viewing the most recent version of this article.
  • previous version (June 18, 2018).

Online Impact

 

Article usage

Select a custom date range for the past year
E.g., 2026-01-17
to
E.g., 2026-01-17

Article usage: June 2018 to January 2026

AbstractFullPdf
Jun 2018335090
Jul 2018788224105
Aug 2018757342
Sep 2018607532
Oct 2018438660
Nov 2018333618
Dec 2018214919
Total 20181355543366
Jan 2019185518
Feb 201912848
Mar 2019127814
Apr 20191111228
May 2019133312
Jun 201965414
Jul 2019145318
Aug 20191212613
Oct 201911479
Nov 2019114015
Dec 20195437
Total 2019125725156
Jan 20203338
Feb 202035421
Mar 202036218
May 202034718
Jun 20201317
Jul 202031195
Aug 20201287
Sep 202054718
Oct 202039016
Nov 20203549
Dec 202071067
Total 202035671134
Jan 20214598
Feb 20213429
Mar 202167217
Apr 20212577
May 202127016
Jun 202128114
Jul 202125012
Aug 20213489
Sep 20210555
Oct 202136729
Nov 202105818
Dec 202116111
Total 202128720155
Jan 20221549
Feb 202214912
Mar 202234610
Apr 20224587
May 202215920
Jun 202226612
Jul 20222393
Aug 202215214
Sep 202232715
Oct 20223439
Nov 20222686
Dec 202213811
Total 202224599128
Jan 20232299
Feb 20230397
Mar 20232419
Apr 202314610
May 20231398
Jun 20230768
Jul 202325517
Aug 2023411825
Sep 20231507
Oct 202318314
Nov 202311687
Dec 2023118325
Total 202326827146
Jan 202455314
Feb 202435313
Mar 202495214
Apr 202455910
May 202431258
Jun 202436311
Jul 20241608
Aug 202446121
Sep 2024115125
Oct 20240696
Nov 20241535
Dec 20242443
Total 202447743138
Jan 202536018
Feb 202528010
Mar 202526913
Apr 202505411
May 202546515
Jun 202537415
Jul 202558122
Aug 202586530
Sep 20251511832
Oct 202599726
Nov 2025139122
Dec 2025139628
Total 202577950242
Jan 202644312
Total 202644312
Total172158211477
Back to top

In this issue

eneuro: 5 (3)
eNeuro
Vol. 5, Issue 3
May/June 2018
  • Table of Contents
  • Index by author
Email

Thank you for sharing this eNeuro article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Using Automated Live Cell Imaging to Reveal Early Changes during Human Motor Neuron Degeneration
(Your Name) has forwarded a page to you from eNeuro
(Your Name) thought you would be interested in this article in eNeuro.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Print
View Full Page PDF
Citation Tools
Using Automated Live Cell Imaging to Reveal Early Changes during Human Motor Neuron Degeneration
Hye Young Shin, Kathleen L. Pfaff, Lance S. Davidow, Chicheng Sun, Takayuki Uozumi, Fumiki Yanagawa, Yoichi Yamazaki, Yasujiro Kiyota, Lee L. Rubin
eNeuro 18 June 2018, 5 (3) ENEURO.0001-18.2018; DOI: 10.1523/ENEURO.0001-18.2018

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Respond to this article
Share
Using Automated Live Cell Imaging to Reveal Early Changes during Human Motor Neuron Degeneration
Hye Young Shin, Kathleen L. Pfaff, Lance S. Davidow, Chicheng Sun, Takayuki Uozumi, Fumiki Yanagawa, Yoichi Yamazaki, Yasujiro Kiyota, Lee L. Rubin
eNeuro 18 June 2018, 5 (3) ENEURO.0001-18.2018; DOI: 10.1523/ENEURO.0001-18.2018
Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Visual Abstract
    • Abstract
    • Significance Statement
    • Introduction
    • Materials and Methods
    • Results
    • Discussion
    • Acknowledgments
    • Footnotes
    • References
    • Synthesis
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Keywords

  • Automated live time-lapse imaging instrument
  • heterogeneity
  • live cell time-lapse imaging
  • morphometric analysis
  • single cell tracking

Responses to this article

Respond to this article

Jump to comment:

No eLetters have been published for this article.

Related Articles

Cited By...

More in this TOC Section

Methods/New Tools

  • A Toolbox of Criteria for Distinguishing Cajal–Retzius Cells from Other Neuronal Types in the Postnatal Mouse Hippocampus
  • Superficial Bound of the Depth Limit of Two-Photon Imaging in Mouse Brain
  • Assessment of Spontaneous Neuronal Activity In Vitro Using Multi-Well Multi-Electrode Arrays: Implications for Assay Development
Show more Methods/New Tools

Novel Tools and Methods

  • Rhythms and Background (RnB): The Spectroscopy of Sleep Recordings
  • The Odor Delivery Optimization Research System (ODORS): An Open-Source Olfactometer for Behavioral Assessments in Tethered and Untethered Rodents
  • Development of a Modified Weight-Drop Apparatus for Closed-Skull, Repetitive Mild Traumatic Brain Injuries in a Mouse Model
Show more Novel Tools and Methods

Subjects

  • Novel Tools and Methods
  • Home
  • Alerts
  • Follow SFN on BlueSky
  • Visit Society for Neuroscience on Facebook
  • Follow Society for Neuroscience on Twitter
  • Follow Society for Neuroscience on LinkedIn
  • Visit Society for Neuroscience on Youtube
  • Follow our RSS feeds

Content

  • Early Release
  • Current Issue
  • Latest Articles
  • Issue Archive
  • Blog
  • Browse by Topic

Information

  • For Authors
  • For the Media

About

  • About the Journal
  • Editorial Board
  • Privacy Notice
  • Contact
  • Feedback
(eNeuro logo)
(SfN logo)

Copyright © 2026 by the Society for Neuroscience.
eNeuro eISSN: 2373-2822

The ideas and opinions expressed in eNeuro do not necessarily reflect those of SfN or the eNeuro Editorial Board. Publication of an advertisement or other product mention in eNeuro should not be construed as an endorsement of the manufacturer’s claims. SfN does not assume any responsibility for any injury and/or damage to persons or property arising from or related to any use of any material contained in eNeuro.