Figure 3. mEPSCs in granule cells from wild-type (WT) and Cln3
Δex1–6 mice are indistinguishable. A, Representative recording of mEPSCs from a granule cell in a culture prepared from WT mice (–60 mV). Traces are consecutive and filtered at 1 kHz for display (mEPSCs are indicated by red dots). B, Same as A but from a granule cell in a culture prepared from Cln3
Δex1–6 mice. Scale bars apply to both A, B. C, upper, Individual mEPSCs from the cell in a, aligned at their point of steepest rise. Middle, Color-coded image of all 77 events. Lower, Averaged mEPSC (black trace) with superimposed SEM (gray fill) and exponential fit to the decay (blue line). The time constant (τdecay) is indicated. D, Same as C but for mEPSCs from the Cln3
Δex1–6 recording in B (scale bars apply to both C, D). E, Pooled data showing similar amplitude and frequency of mEPSCs in granule cells from WT and Cln3
Δex1–6 mice. Left, Cumulative probability distributions for mEPSC amplitudes. The averaged distributions are shown in bold (WT blue; Cln3
Δex1–6 red). Right, Box-and-whisker plots (as in Fig. 1) for mEPSC frequency (log10 scale) and amplitude (n.s., non-significant; Wilcoxon rank sum test). F, left, Representative current-variance relationships. The dashed line indicates the background current variance. The single-channel conductance (γ) was calculated from the weighted-mean unitary current estimated from the parabolic fit. Right, Box-and-whisker plots (as in E) showing similar values for conductance. G, Representative recordings from cultured granule cells at −60 and +60 mV with corresponding count-matched averaged mEPSCs (see Materials and Methods). Traces are from a WT cell (left) and a Cln3
Δex1–6 cell (right). Far right, Box-and-whisker plots (as in E) showing pooled data for count-matched rectification index (RICM).