1E | Unpaired t test, two-tailed | TMPase: N = 6 | TMPase: p < 0.0001 |
Cell area | | CGRP: N = 5 | CGRP+,NF200-: p < 0.0001 |
| | | CGRP+,NF200+: p = 0.4803 |
Table 3 | Unpaired t test, two-tailed | N = 5–6 | See Table 3 |
Cell distribution | | | |
2B | Unpaired t test, two-tailed | Adult: N = 6 | Adult control vs adult Nav-Tsc2: p = 0.0047 |
Axon diameter | | P29: N = 5 | P29 control vs P29 Nav-Tsc2: p = 0.0442 |
| | | Adult control vs P29 control: p = 0.318 |
| | | Adult Nav-Tsc2 vs P29 Nav-Tsc2: p = 0.0258 |
2C | Unpaired t test, two-tailed | Adult: N = 6 | Adult control vs adult Nav-Tsc2: p = 0.0055 |
Axons >1 micron | | P29: N = 5 | P29 control vs P29 Nav-Tsc2: p = 0.2076 |
| | | Adult control vs P29 control: p = 0.4211 |
| | | Adult Nav-Tsc2 vs P29 Nav-Tsc2: p = 0.0125 |
2D | Unpaired t test, two-tailed | Adult: N = 6 | Adult control vs adult Nav-Tsc2: p = 0.0371 |
Axons/bundle | | P29: N = 5 | P29 control vs P29 Nav-Tsc2: p = 0.0747 |
| | | Adult control vs P29 control: p = 0.5018 |
| | | Adult Nav-Tsc2 vs P29 Nav-Tsc2: p = 0.0202 |
3C | Unpaired t test, two-tailed | N = 5 | TuJ1: p < 0.0001 |
Skin innervation | | | |
3F | Unpaired t test, two-tailed | N = 5 | Lamina I: p = 0.0403 |
GFP density | | | Lamina II: p = 0.0002 |
5C,F,G,L | Unpaired t test, two-tailed | N = 5 | Total neurons: p = 0.081 |
DRG neuron | | SP: N = 6 | NF200 total: p = 0.314 |
counting | | | SP total: p = 0.0006 |
| | | CGRP+,NF200-: p < 0.0001 |
| | | CGRP+,NF200+: p = 0.0007 |
| | | TrkA+,NF200-: p < 0.0001 |
| | | TrkA+,NF200+: p = 0.0005 |
| | | IB4 total: p = 0.0001 |
| | | IB4+,NF200+: p = 0.0037 |
6C | Unpaired t test, two-tailed | N = 4 whole DRG | Nav1.8: p = 0.0613 |
qPCR of | | N = 3 FACS-sorted samples | TrkA: p = 0.0015 |
FACS-sorting | | | TrkB: p = 0.9758 |
| | | TrkC: p = 0.0020 |
| | | Prx: p = 0.1784 |
| | | Egr2: p = 0.5834 |
8A | Unpaired t test, two-tailed | Female: N = 13 | Female: p = 0.2513 |
von Frey | | Male: N = 12 | Male: p = 0.462 |
8B | Unpaired t test, two-tailed | Female: N = 6 control, N = 4 cKO | Female: p = 0.2390 |
Cold plantar | | male: N = 11 control, N = 10 cKO | Male: p = 0.2102 |
8C | Unpaired t test, two-tailed | Female: N = 13 | Female: p = 0.0046 |
Hargreaves | | Male: N = 9 | Male: p = 0.0337 |
8D | Two-way RM ANOVA | Control: N = 9 | Interaction: F(5,75) = 1.82; p = 0.1191 |
Female CCI | | Nav-Tsc2: N = 8 | Time: F(5,75) = 10.04; p < 0.0001 |
| | | Genotype: F(1,15) = 9.032; p = 0.0089 |
8D | Sidak’s multiple | Control: N = 9 | BL: adjusted p > 0.9999 |
Female CCI | comparison test | Nav-Tsc2: N = 8 | POD 4: adjusted p = 0.269 |
| | | POD 6: adjusted p = 0.9896 |
| | | POD 20: adjusted p = 0.9209 |
| | | POD 27: adjusted p = 0.0071 |
| | | POD 34: adjusted p = 0.0836 |
8E | Two-way RM ANOVA | Control: N = 11 | Interaction: F(5,75) = 0.7719; p = 0.5723 |
Male CCI | | Nav-Tsc2: N = 10 | Time: F(5,95) = 4.873; p = 0.0005 |
| | | Genotype: F(1,19) = 3.403; p = 0.0807 |
8E | Sidak’s multiple | Control: N = 11 | BL: adjusted p > 0.9999 |
Male CCI | comparison test | Nav-Tsc2: N = 10 | POD 4: adjusted p = 0.9992 |
| | | POD 6: adjusted p = 0.9998 |
| | | POD 20: adjusted p = 0.3652 |
| | | POD 27: adjusted p = 0.236 |
| | | POD 34: adjusted p = 0.8045 |
8F | Two-way RM ANOVA | Control: N = 17 | Interaction: F(4,128) = 1.03; p = 0.3946 |
Rotarod | | Nav-Tsc2: N = 17 | Trial number: F(4,128) = 60.26; p < 0.0001 |
| | | Genotype: F(1,32) = 4.08; p = 0.0518 |
8F | Sidak’s multiple | Control: N = 17 | Trial 1: adjusted p = 0.8576 |
Rotarod | comparison test | Nav-Tsc2: N = 17 | Trial 2: adjusted p = 0.8396 |
| | | Trial 3: adjusted p = 0.2686 |
| | | Trial 4: adjusted p = 0.0812 |
| | | Trial 5: adjusted p = 0.1971 |
8G | Unpaired t test, two-tailed | Control: N = 23 | p = 0.8027 |
Open field activity | | Nav-Tsc2: N = 23 | |
8H | Two-way RM ANOVA | Control: N = 23 | Interaction: F(1,43) = 2.62; p = 0.1129 |
Pole climb down | | Nav-Tsc2: N = 22 | Trial number: F(1,43) = 4.516; p = 0.0394 |
| | | Genotype: F(1,43) = 0.04254; p = 0.8376 |
8H | Sidak’s multiple | Control: N = 23 | Trial 1: adjusted p = 0.84 |
Pole climb down | comparison test | Nav-Tsc2: N = 22 | Trial 2: adjusted p = 0.606 |