Skip to main content

Umbrella menu

  • SfN.org
  • eNeuro
  • The Journal of Neuroscience
  • Neuronline
  • BrainFacts.org

Main menu

  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Latest Articles
    • Issue Archive
    • Editorials
    • Research Highlights
  • TOPICS
    • Cognition and Behavior
    • Development
    • Disorders of the Nervous System
    • History, Teaching and Public Awareness
    • Integrative Systems
    • Neuronal Excitability
    • Novel Tools and Methods
    • Sensory and Motor Systems
  • ALERTS
  • FOR AUTHORS
  • EDITORIAL BOARD
  • BLOG
  • ABOUT
    • Overview
    • For the Media
    • Privacy Policy
    • Contact Us
    • Feedback
  • SfN.org
  • eNeuro
  • The Journal of Neuroscience
  • Neuronline
  • BrainFacts.org

User menu

  • My alerts

Search

  • Advanced search
eNeuro
  • My alerts

eNeuro

Advanced Search

Submit a Manuscript
  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Latest Articles
    • Issue Archive
    • Editorials
    • Research Highlights
  • TOPICS
    • Cognition and Behavior
    • Development
    • Disorders of the Nervous System
    • History, Teaching and Public Awareness
    • Integrative Systems
    • Neuronal Excitability
    • Novel Tools and Methods
    • Sensory and Motor Systems
  • ALERTS
  • FOR AUTHORS
  • EDITORIAL BOARD
  • BLOG
  • ABOUT
    • Overview
    • For the Media
    • Privacy Policy
    • Contact Us
    • Feedback
PreviousNext
Research ArticleNew Research, Novel Tools and Methods

Measuring and Validating the Levels of Brain-Derived Neurotrophic Factor in Human Serum

Yvonne Naegelin, Hayley Dingsdale, Katharina Säuberli, Sabine Schädelin, Ludwig Kappos and Yves-Alain Barde
eNeuro 12 March 2018, 5 (2) ENEURO.0419-17.2018; DOI: https://doi.org/10.1523/ENEURO.0419-17.2018
Yvonne Naegelin
1School of Biosciences, Cardiff University, Cardiff CF10 3AX, United Kingdom
2Department of Neurology, University Hospital Basel, Basel 4031, Switzerland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hayley Dingsdale
1School of Biosciences, Cardiff University, Cardiff CF10 3AX, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Katharina Säuberli
1School of Biosciences, Cardiff University, Cardiff CF10 3AX, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sabine Schädelin
3Clinical Trial Unit, Department of Clinical Research, University Hospital Basel, Basel 4031, Switzerland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ludwig Kappos
2Department of Neurology, University Hospital Basel, Basel 4031, Switzerland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yves-Alain Barde
1School of Biosciences, Cardiff University, Cardiff CF10 3AX, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Yves-Alain Barde
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Article Figures & Data

Figures

  • Tables
  • Figure1
    • Download figure
    • Open in new tab
    • Download powerpoint
  • Figure 1.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 1.

    BDNF serum levels in a healthy cohort at Initial (n = 259) and 12-month (n = 226) visit. Measurements of the same subject are connected by a colored line to indicate percentage change in BDNF values between visits. Samples are separated on the x-axis by age in years.

  • Figure 2.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 2.

    BDNF serum levels and age of participant. The red broken line indicates the estimated serum BDNF for a given age in an average volunteer.

  • Figure 3.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 3.

    Correlation between BDNF values and platelet counts. A, BDNF values per platelet plotted against age. B, The red broken line indicates the estimated BDNF for a given platelet count in an average volunteer.

  • Figure 4.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 4.

    Correlation between BDNF values and hematocrit. The red broken line indicates the estimated BDNF for a given hematocrit in an average volunteer.

  • Figure 5.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 5.

    Cohort size estimation as a function of expected difference between two populations. Thick gray lines indicate a power of 80%; fine gray lines indicate a power of 90%. A, Cohort size required for a 20% difference in BDNF values. B, Cohort size required for a 10% difference in BDNF values.

  • Figure 6.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 6.

    Comparison of mean ELISA values with Western blotting determinations of the same serum samples. A, Western blotting used to quantify BDNF in two different serum samples (1 μl each) against a standard curve generated from three different quantities of recombinant BDNF with added BSA. B, Comparison between mean values obtained by Western blotting versus those obtained by ELISA. Error bars show SD, derived from at least three separate blots (WB) or three replicate wells in the same plate (ELISA).

Tables

  • Figures
    • View popup
    Table 1.

    Cohort characteristics at Initial (n = 259) and 12-month visit (n = 226)

    Visit 1Visit 2
    N259226
    Age in years [mean (SD)]44.31 (11.26)46.11 (10.80)
    Sex [males (%)]81 (31.3)74 (32.7)
    Time between samples in months [mean (SD)]12.87 (2.21)
    • View popup
    Table 2.

    Summary statistics for BDNF values in serum separated by gender

    VariableGenderNMean (ng/ml)SD (ng/ml)p
    BDNF V1F17832.858.570.7
    M8132.347.82
    BDNF V2F15232.988.470.99
    M7432.958.19
Back to top

In this issue

eneuro: 5 (2)
eNeuro
Vol. 5, Issue 2
March/April 2018
  • Table of Contents
  • Index by author
Email

Thank you for sharing this eNeuro article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Measuring and Validating the Levels of Brain-Derived Neurotrophic Factor in Human Serum
(Your Name) has forwarded a page to you from eNeuro
(Your Name) thought you would be interested in this article in eNeuro.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Print
View Full Page PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Citation Tools
Measuring and Validating the Levels of Brain-Derived Neurotrophic Factor in Human Serum
Yvonne Naegelin, Hayley Dingsdale, Katharina Säuberli, Sabine Schädelin, Ludwig Kappos, Yves-Alain Barde
eNeuro 12 March 2018, 5 (2) ENEURO.0419-17.2018; DOI: 10.1523/ENEURO.0419-17.2018

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Respond to this article
Share
Measuring and Validating the Levels of Brain-Derived Neurotrophic Factor in Human Serum
Yvonne Naegelin, Hayley Dingsdale, Katharina Säuberli, Sabine Schädelin, Ludwig Kappos, Yves-Alain Barde
eNeuro 12 March 2018, 5 (2) ENEURO.0419-17.2018; DOI: 10.1523/ENEURO.0419-17.2018
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Visual Abstract
    • Abstract
    • Significance Statement
    • Introduction
    • Materials and Methods
    • Results
    • Discussion
    • Footnotes
    • References
    • Synthesis
    • Author Response
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Keywords

  • antibodies
  • BDNF
  • Biomarkers
  • ELISA
  • Platelets
  • Western blotting

Responses to this article

Respond to this article

Jump to comment:

No eLetters have been published for this article.

Related Articles

Cited By...

More in this TOC Section

New Research

  • SRF is required for maintenance of astrocytes in non-reactive state in the mammalian brain
  • Mapping sex-specific neurodevelopmental alterations in neurite density and morphology in a rat genetic model of psychiatric illness
  • Lytic cell death in specific microglial subsets is required for preventing atypical behavior in mice
Show more New Research

Novel Tools and Methods

  • Citric Acid Water as an Alternative to Water Restriction for High-Yield Mouse Behavior
  • Using head-mounted ethanol sensors to monitor olfactory information and determine behavioral changes associated with ethanol-plume contact during mouse odor-guided navigation
  • P2X-GCaMPs as versatile tools for imaging extracellular ATP signaling
Show more Novel Tools and Methods

Subjects

  • Novel Tools and Methods
  • Home
  • Alerts
  • Visit Society for Neuroscience on Facebook
  • Follow Society for Neuroscience on Twitter
  • Follow Society for Neuroscience on LinkedIn
  • Visit Society for Neuroscience on Youtube
  • Follow our RSS feeds

Content

  • Early Release
  • Current Issue
  • Latest Articles
  • Issue Archive
  • Blog
  • Browse by Topic

Information

  • For Authors
  • For the Media

About

  • About the Journal
  • Editorial Board
  • Privacy Policy
  • Contact
  • Feedback
(eNeuro logo)
(SfN logo)

Copyright © 2021 by the Society for Neuroscience.
eNeuro eISSN: 2373-2822

The ideas and opinions expressed in eNeuro do not necessarily reflect those of SfN or the eNeuro Editorial Board. Publication of an advertisement or other product mention in eNeuro should not be construed as an endorsement of the manufacturer’s claims. SfN does not assume any responsibility for any injury and/or damage to persons or property arising from or related to any use of any material contained in eNeuro.