Figure 6-1
Amino acid sequence alignment of wasp, bee, and ant HsTRPA channels and the positions of ankyrin repeats, transmembrane segments, and selectivity filter. HsTRPA channels of N. vitripennis (jewel wasp), Megachile rotundata (leafcutter bee), Apis mellifera (western honey bee), Bombus terrestris (buff-tailed bumble bee), Harpegnathos saltator (Jerdon’s jumping ant), Camponotus floridanus (Florida carpenter ant), Linepithema humile (Argentine ant), Pogonomyrmex barbatus (red harvester ant), Solenopsis invicta (red imported fire ant), Acromyrmex echinatior (Panamanian leafcutter ant), and Atta cephalotes (leafcutter ant) were aligned by MUSCLE. The extended N-terminal sequences of MrHsTRPA, AmHsTRPA, and BtHsTRPA channels are highlighted with gray. Odd and even numbered ankyrin repeats (ARs1–7) in AmHsTRPA and SiHsTRPA are highlighted by yellow and green, respectively. The transmembrane segments (S1–6) and selectivity filter (SF) are highlighted by blue and purple, respectively. Identical amino acids between the 11 channels are indicated by asterisks, and the similar amino acids are shown by either . or :. Download Figure 6-1, DOCX file.