Article Figures & Data
Figures
- Figure 1.
A, Overview of experimental strategy. Cell types were genetically tagged with a fluorophore to target patch-clamp recordings. During whole-cell recordings, 5-s-long oscillatory current at fixed frequencies were injected into the cells to obtain an action potential output corresponding to each frequency. B, Individual example raw traces of single cells of the investigated cell types at three different input frequencies.
- Figure 2.
Effects of input oscillation frequency on LH cell population firing rates (spike output, red) and impedances (blue). Bandwidth bars (purple) denote oscillation frequencies at which there was significant (non-zero) spike output (calculated by one-sample t tests and corrected for multiple comparisons by controlling the false discovery rate, see Materials and Methods). Values are mean ± SEM. Cell numbers for MCH, orexin, GAD65, and VGAT neurons are 14, 13, 17, and 16, respectively.
- Figure 3.
A, Effect of input oscillation frequency on LH cell population spike outputs (same spike data as in Fig. 2, but normalized to the maximum spike output in each cell, to facilitate comparisons of oscillation frequency effects between cell classes). Values are mean ± SEM. B, Differences in population spike outputs across oscillation frequencies. The y-axis shows adjusted p values of a two-way repeated-measures ANOVA with Tuckey’s multiple comparison correction, for the four cell types and 14 input oscillation frequencies (ANOVA: interaction, F(36,672) = 1.907; p = 0.0013). C, Effect of input oscillation frequency on LH cell population membrane impedances (same impedance data as in Fig. 2, but normalized to the maximum impedance in each cell). Values are mean ± SEM.
- Figure 4.
A, left, Pie charts depicting the percentage of actively-tuned cells (cells whose normalized spike frequency significantly differs from its normalized impedance magnitude), and passively-tuned cells (cells whose normalized spike frequency did not significantly differ from its normalized impedance magnitude). To group neurons into these categories, firing and impedance profiles of each individual cell were compared using a paired t test with correction for multiple comparisons by controlling the false discovery rate (two-stage step-up method of Benjamini, Krieger, and Yekutieli). Middle, Examples of individual actively-tuned cells. Right, Examples of individual passively-tuned cells. B, Activeness of cell populations (statistical difference between normalized spike frequency and normalized impedance of each cell type, across input oscillation frequencies, n numbers for each cell type are as indicated in A. The y-axis shows adjusted p-values from paired t tests with correction for multiple comparisons by controlling the false discovery rate (two-stage step-up method of Benjamini, Krieger, and Yekutieli).
Tables
MCH Orexin GAD65 VGAT Membrane resistance (MΩ) 474.8571 ± 54.2614, n = 14 463.0385 ± 56.76324, n = 13 619.2471 ± 49.09955, n = 17 724.4438 ± 92.54686, n = 16 Membrane time constant (ms) 40.60857 ± 6.530006, n = 14 43.24915 ± 7.335909, n = 13 32.42376 ± 4.217719, n = 17 32.54188 ± 3.505608, n = 16
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