Figure 3. BDNF expression and protein synthesis in the Prh are required for consolidation of similar, but not dissimilar, object memory representations. A, Schematic illustration of the two configurations of the SOR task depicting the time point at which BDNF-ASO was infused. B, BDNF protein levels in the Prh of nontrained animals infused with either BDNF-ASO or BDNF-MSO 2 h before injection of kainic acid into the Prh. Unpaired t test (t = 2.334), p = 0.0322, n = 9–10, d = 0.377. C, Effect of BDNF-ASO or BDNF-MSO injections on the discrimination ratios for the similar (s-SOR) and the dissimilar (d-SOR) version of the task. Paired t test (t = 4.284), p = 0.0036, n = 8–13, d = 2.284. Inset, percentage of time spent exploring each of the objects in the sample phase in the s-SOR (left) and d-SOR (right), 2 h after BDNF-MSO (light color) or BDNF-MSO (dark color). Similar: repeated-measures two-way ANOVA; F = 0.652, p(drug) = 0.440, F = 0.957, p(object) = 0.403, F = 0.135, p(interaction) = 0.875. Dissimilar: repeated-measures two-way ANOVA; F = 0.055, p(drug) = 0.818, F = 1.388, p(object) = 0.269, F = 0.001, p(interaction) = 0.999. D, The injection of emetine in the PRH 15 min before the sample phase impaired performance on the s-SOR task during the choice phase 24 h later relative to vehicle-injected rats (left), whereas there was no effect of emetine on the d-SOR version of the task (right). Paired t test (s-SOR, t = 3.540), p = 0.0076, n = 9, d = 1.698; paired t test (d-SOR, t = 1.284), p = 0.231, n = 10. Data are expressed as the mean ± SEM; *, p < 0.05; **, p < 0.01.