Figure 1. Schematic overview of the experimental design in this study. A, Timeline for experimental series in which neuroendocrine data were collected (A1), either at PND26, PND29, or PND90. Animals were exposed to 24-h MD or control treatment at PND3. Part of the animals were treated twice daily with MIF or VEH administered through oral gavage directly into the stomach. At the indicated moment, we monitored body weight, adrenal weight (only at PND29), CORT levels, and collected hippocampal lobes for later Western blotting for MR and GR (typical examples shown in A2). B, Timeline for the object-in-(novel) context testing (B1). The details of the task are shown in B2, see Materials and Methods for details. C, Schematic representation of the rIGT maze model. D, Anatomic localization of brain region used for analysis of c-Fos expression. Bregma coordinates are indicated above each coronal section. DG, dentate gyrus; CA1, CA1 region of the hippocampus; CA3, CA3 region of the hippocampus; DSL, dorsolateral striatum; DMS, dorsomedial striatum; NaC, nucleus accumbens core; NaS, nucleus accubmens shell; Cg1, cingulate cortex; PrL, prelimbic cortex; IL, infralimbic cortex; INS, insular cortex; medOFC, medial orbital frontal cortex (OFC); latOFC, lateral OFC; venOFC, ventral OFC. E, Timeline for the electrophysiological recordings of CA1 pyramidal cells in hippocampal slices (E1). Typical example traces of mEPSCs are shown in E2, at a low time resolution (left) and a high time resolution (right). E3, Anatomic localization of brain region used for mEPSCs recording.