Figure 2-1
NCS-Rapgef2 protein expression in mouse brain. A, Characterization of Rapgef2 antibody NNLE2 by immunohistochemistry. Upper panel, Mouse cortex stained for NCS-Rapgef2 with NNLE-2 antibody in the absence (left) or presence (right) of the blocking peptide. Lower panel, NCS-Rapgef2 staining with NNLE-2 antibody four weeks after the NAc on one side of the brain of floxed Rapgef2 mouse Rapgef2cko/cko was injected with AAV9.hSyn.HI.GFP-Cre virus. Scale bar: 20 µm. B, Western blottings using tissues from different areas of CNS showed high level of NCS-Rapgef2 protein expression in hippocampus, cortex, striatum, etc. C, Expression of NCS-Rapgef2 protein in C57BL6N brain. Immunohistochemistry using Rapgef2 N-terminal-specific antibody NNLE-2 indicated that Rapgef2 protein (red) is highly expressed in the neurons of cortex, hippocampus, striatum, amygdala, and other brain regions. D, NCS-Rapgef2 protein is predominantly expressed in the excitatory neurons of corticolimbic brain areas indicated by none overlapping staining of Rapgef2 and inhibitory neuron marker GAD67 in these areas. NCS-Rapgef2 protein is highly expressed in medial spiny neurons in striatum indicated by localization of Rapgef2 in the neurons with weak GAD67 staining in striatum. Immunostaining of NCS-Rapgef2 is not localized in astrocytes as indicated by GFAP staining. Download Figure 2-1, TIF file.