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Research ArticleMethods/New Tools, Novel Tools and Methods

Aberrant Cortical Activity in Multiple GCaMP6-Expressing Transgenic Mouse Lines

Nicholas A. Steinmetz, Christina Buetfering, Jerome Lecoq, Christian R. Lee, Andrew J. Peters, Elina A. K. Jacobs, Philip Coen, Douglas R. Ollerenshaw, Matthew T. Valley, Saskia E. J. de Vries, Marina Garrett, Jun Zhuang, Peter A. Groblewski, Sahar Manavi, Jesse Miles, Casey White, Eric Lee, Fiona Griffin, Joshua D. Larkin, Kate Roll, Sissy Cross, Thuyanh V. Nguyen, Rachael Larsen, Julie Pendergraft, Tanya Daigle, Bosiljka Tasic, Carol L. Thompson, Jack Waters, Shawn Olsen, David J. Margolis, Hongkui Zeng, Michael Hausser, Matteo Carandini and Kenneth D. Harris
eNeuro 4 September 2017, 4 (5) ENEURO.0207-17.2017; https://doi.org/10.1523/ENEURO.0207-17.2017
Nicholas A. Steinmetz
1UCL Institute of Neurology, University College London, London, UK
2UCL Institute of Ophthalmology, University College London, London, UK
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Christina Buetfering
3Department of Neuroscience, Physiology, and Pharmacology, University College London, London, UK
4Wolfson Institute for Biomedical Research, University College London, London, UK
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Jerome Lecoq
5 Allen Institute for Brain Science, Seattle, WA
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Christian R. Lee
6Department of Cell Biology and Neuroscience, Rutgers, the State University of New Jersey, Piscataway, NJ
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Andrew J. Peters
2UCL Institute of Ophthalmology, University College London, London, UK
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Elina A. K. Jacobs
1UCL Institute of Neurology, University College London, London, UK
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Philip Coen
1UCL Institute of Neurology, University College London, London, UK
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Douglas R. Ollerenshaw
5 Allen Institute for Brain Science, Seattle, WA
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Matthew T. Valley
5 Allen Institute for Brain Science, Seattle, WA
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Saskia E. J. de Vries
5 Allen Institute for Brain Science, Seattle, WA
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Marina Garrett
5 Allen Institute for Brain Science, Seattle, WA
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Jun Zhuang
5 Allen Institute for Brain Science, Seattle, WA
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Peter A. Groblewski
5 Allen Institute for Brain Science, Seattle, WA
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Sahar Manavi
5 Allen Institute for Brain Science, Seattle, WA
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Jesse Miles
5 Allen Institute for Brain Science, Seattle, WA
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Casey White
5 Allen Institute for Brain Science, Seattle, WA
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Eric Lee
5 Allen Institute for Brain Science, Seattle, WA
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Fiona Griffin
5 Allen Institute for Brain Science, Seattle, WA
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Joshua D. Larkin
5 Allen Institute for Brain Science, Seattle, WA
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Kate Roll
5 Allen Institute for Brain Science, Seattle, WA
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Sissy Cross
5 Allen Institute for Brain Science, Seattle, WA
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Thuyanh V. Nguyen
5 Allen Institute for Brain Science, Seattle, WA
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Rachael Larsen
5 Allen Institute for Brain Science, Seattle, WA
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Julie Pendergraft
5 Allen Institute for Brain Science, Seattle, WA
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Tanya Daigle
5 Allen Institute for Brain Science, Seattle, WA
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Bosiljka Tasic
5 Allen Institute for Brain Science, Seattle, WA
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Carol L. Thompson
5 Allen Institute for Brain Science, Seattle, WA
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Jack Waters
5 Allen Institute for Brain Science, Seattle, WA
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Shawn Olsen
5 Allen Institute for Brain Science, Seattle, WA
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David J. Margolis
6Department of Cell Biology and Neuroscience, Rutgers, the State University of New Jersey, Piscataway, NJ
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Hongkui Zeng
5 Allen Institute for Brain Science, Seattle, WA
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Michael Hausser
3Department of Neuroscience, Physiology, and Pharmacology, University College London, London, UK
4Wolfson Institute for Biomedical Research, University College London, London, UK
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Matteo Carandini
2UCL Institute of Ophthalmology, University College London, London, UK
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Kenneth D. Harris
1UCL Institute of Neurology, University College London, London, UK
3Department of Neuroscience, Physiology, and Pharmacology, University College London, London, UK
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Article Information

DOI 
https://doi.org/10.1523/ENEURO.0207-17.2017
PubMed 
28932809
Published By 
Society for Neuroscience
History 
  • Received June 13, 2017
  • Revision received August 8, 2017
  • Accepted August 14, 2017
  • Published online September 4, 2017.
Copyright & Usage 
Copyright © 2017 Steinmetz et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Nicholas A. Steinmetz1,2,
  2. Christina Buetfering3,4,
  3. Jerome Lecoq5,
  4. Christian R. Lee6,
  5. Andrew J. Peters2,
  6. Elina A. K. Jacobs1,
  7. Philip Coen1,
  8. Douglas R. Ollerenshaw5,
  9. Matthew T. Valley5,
  10. Saskia E. J. de Vries5,
  11. Marina Garrett5,
  12. Jun Zhuang5,
  13. Peter A. Groblewski5,
  14. Sahar Manavi5,
  15. Jesse Miles5,
  16. Casey White5,
  17. Eric Lee5,
  18. Fiona Griffin5,
  19. Joshua D. Larkin5,
  20. Kate Roll5,
  21. Sissy Cross5,
  22. Thuyanh V. Nguyen5,
  23. Rachael Larsen5,
  24. Julie Pendergraft5,
  25. Tanya Daigle5,
  26. Bosiljka Tasic5,
  27. Carol L. Thompson5,
  28. Jack Waters5,
  29. Shawn Olsen5,
  30. David J. Margolis6,
  31. Hongkui Zeng5,
  32. Michael Hausser3,4,
  33. Matteo Carandini2 and
  34. Kenneth D. Harris1,3
  1. 1UCL Institute of Neurology, University College London, London, UK
  2. 2UCL Institute of Ophthalmology, University College London, London, UK
  3. 3Department of Neuroscience, Physiology, and Pharmacology, University College London, London, UK
  4. 4Wolfson Institute for Biomedical Research, University College London, London, UK
  5. 5 Allen Institute for Brain Science, Seattle, WA
  6. 6Department of Cell Biology and Neuroscience, Rutgers, the State University of New Jersey, Piscataway, NJ
  1. Correspondence should be addressed to Nicholas A. Steinmetz. Wing 2.1, Crucifrom Building University College London London WC1E 6AE. E-mail: nick.steinmetz{at}gmail.com.
View Full Text

Author contributions

  1. Author contributions: N.A.S., C.B., J.L., C.R.L., A.J.P., E.A.K.J., D.R.O., M.T.V., S.E.J.d.V., M.G., P.A.G., J.P., B.T., J.W., S.O., D.J.M., H.Z., M.H., M.C., and K.D.H. designed research; N.A.S., C.B., C.R.L., A.J.P., E.A.K.J., P.C., D.R.O., M.T.V., M.G., J.Z., S.M., J.M., C.W., E.L., F.G., J.D.L., K.R., S.C., T.V.N., R.L., J.P., and D.J.M. performed research; N.A.S., C.B., J.L., C.R.L., A.J.P., D.R.O., M.T.V., S.E.J.d.V., M.G., C.W., E.L., F.G., J.D.L., K.R., S.C., T.V.N., J.P., T.D., C.L.T., D.J.M., and K.D.H. analyzed data; N.A.S., C.B., J.L., C.R.L., A.J.P., E.A.J., D.R.O., M.V., S.E.J.d.V., M.G., B.T., C.T., J.W., D.J.M., M.C., and K.D.H. wrote the paper.

View Abstract

Disclosures

  • Authors report no conflict of interest.

  • EC | Horizon 2020 (EU Framework Programme for Research and Innovation); Human Frontier Science Program (HFSP); European Molecular Biology Organization (EMBO); Wellcome; Biotechnology and Biological Sciences Research Council (BBSRC); EC | European Research Council (ERC); New Jersey Commission on Brain Injury Research (NJCBIR); National Science Foundation (NSF); HHS | National Institutes of Health (NIH). N.A.S. was supported by postdoctoral fellowships from the HFSP and the Marie Curie Action of the EU. C.B. was supported by an EMBO Long-Term Fellowship and the Marie Curie Action of the EU. M.H. was supported by the Wellcome Trust, BBSRC, and ERC. D.J.M. was supported by the NJCBIR, the NSF, and NIH. M.C. holds the GlaxoSmithKline / Fight for Sight Chair in Visual Neuroscience.

Funding

  • EC | Horizon 2020 (EU Framework Programme for Research and Innovation)

  • Human Frontier Science Program (HFSP)

  • European Molecular Biology Organization (EMBO)

  • Wellcome

  • Biotechnology and Biological Sciences Research Council (BBSRC)

  • EC | European Research Council (ERC)

  • New Jersey Commission on Brain Injury Research (NJCBIR)

  • National Science Foundation (NSF)

  • HHS | National Institutes of Health (NIH)

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  • previous version (September 04, 2017).

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Sep 201718083238997
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Nov 2017212395277
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Jan 201862919213
Feb 20185616795
Mar 201857142101
Apr 2018107311264
May 20183719386
Jun 201885343286
Jul 20183213575
Aug 201875276256
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Feb 20192017852
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Apr 2019713659
May 2019611651
Jun 20191321567
Jul 20191127571
Aug 20191826389
Oct 20191227281
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May 20201018185
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Jul 20201520349
Aug 20201014242
Sep 20201822779
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Total 20201352295613
Jan 2021823764
Feb 2021614136
Mar 202110223691
Apr 20211919551
May 20211621551
Jun 20211023768
Jul 2021618247
Aug 2021318551
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Total 20211902432740
Jan 20221323269
Feb 2022532560
Mar 20221223070
Apr 2022517652
May 2022021265
Jun 2022517535
Jul 2022716137
Aug 2022216436
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Total 2022832242617
Jan 2023316545
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Total 20232212234520
Jan 202449191101
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Apr 20243134860
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Jun 20242127034
Jul 20241936469
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Sep 20242034655
Oct 20243033458
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Total 20243123318817
Jan 20252522750
Feb 20252326549
Mar 20251323042
Apr 20253515255
May 20251816251
Jun 20255821575
Jul 20255123285
Aug 20255119279
Sep 20253021069
Oct 20253335066
Nov 20252933966
Dec 20253224171
Total 20253982815758
Jan 20264133567
Feb 20262528638
Mar 20261412114
Total 202680742119
Total4618286338295
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Aberrant Cortical Activity in Multiple GCaMP6-Expressing Transgenic Mouse Lines
Nicholas A. Steinmetz, Christina Buetfering, Jerome Lecoq, Christian R. Lee, Andrew J. Peters, Elina A. K. Jacobs, Philip Coen, Douglas R. Ollerenshaw, Matthew T. Valley, Saskia E. J. de Vries, Marina Garrett, Jun Zhuang, Peter A. Groblewski, Sahar Manavi, Jesse Miles, Casey White, Eric Lee, Fiona Griffin, Joshua D. Larkin, Kate Roll, Sissy Cross, Thuyanh V. Nguyen, Rachael Larsen, Julie Pendergraft, Tanya Daigle, Bosiljka Tasic, Carol L. Thompson, Jack Waters, Shawn Olsen, David J. Margolis, Hongkui Zeng, Michael Hausser, Matteo Carandini, Kenneth D. Harris
eNeuro 4 September 2017, 4 (5) ENEURO.0207-17.2017; DOI: 10.1523/ENEURO.0207-17.2017

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Aberrant Cortical Activity in Multiple GCaMP6-Expressing Transgenic Mouse Lines
Nicholas A. Steinmetz, Christina Buetfering, Jerome Lecoq, Christian R. Lee, Andrew J. Peters, Elina A. K. Jacobs, Philip Coen, Douglas R. Ollerenshaw, Matthew T. Valley, Saskia E. J. de Vries, Marina Garrett, Jun Zhuang, Peter A. Groblewski, Sahar Manavi, Jesse Miles, Casey White, Eric Lee, Fiona Griffin, Joshua D. Larkin, Kate Roll, Sissy Cross, Thuyanh V. Nguyen, Rachael Larsen, Julie Pendergraft, Tanya Daigle, Bosiljka Tasic, Carol L. Thompson, Jack Waters, Shawn Olsen, David J. Margolis, Hongkui Zeng, Michael Hausser, Matteo Carandini, Kenneth D. Harris
eNeuro 4 September 2017, 4 (5) ENEURO.0207-17.2017; DOI: 10.1523/ENEURO.0207-17.2017
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Keywords

  • cortex
  • epilepsy
  • GCaMP
  • transgenic

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Methods/New Tools

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Show more Methods/New Tools

Novel Tools and Methods

  • Superficial Bound of the Depth Limit of Two-Photon Imaging in Mouse Brain
  • A Toolbox of Criteria for Distinguishing Cajal–Retzius Cells from Other Neuronal Types in the Postnatal Mouse Hippocampus
  • Assessment of Spontaneous Neuronal Activity In Vitro Using Multi-Well Multi-Electrode Arrays: Implications for Assay Development
Show more Novel Tools and Methods

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