Figure 6-1
Development of a TetO-UPRT mouse with neuronal expression responsive to DOX. A, Figure of the TetO-UPRT transgene. B, HA-staining for UPRT before and after DOX administration (scale bar: 1000 μm, 5× objective). Both images were taken with an exposure time of 150 ms and were adjusted identically for brightness and contrast (Photoshop-adjusted brightness: 30, contrast: 100). DAPI is also shown to highlight underlying cell structure. Mice were placed on high DOX (2 μg/ml administered in 1% sucrose water) for one week. The transgene was DOX responsive, with a substantial reduction in UPRT protein expression after one week of DOX administration, although a small amount of leaky expression remained. Placing mice on high DOX from birth may help with leaky expression. Download Figure 6-1, TIFF file.