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Research ArticleNew Research, Novel Tools and Methods

TU-Tagging: A Method for Identifying Layer-Enriched Neuronal Genes in Developing Mouse Visual Cortex

Johanna Tomorsky, Leah DeBlander, Clifford G. Kentros, Chris Q. Doe and Cristopher M. Niell
eNeuro 19 September 2017, 4 (5) ENEURO.0181-17.2017; https://doi.org/10.1523/ENEURO.0181-17.2017
Johanna Tomorsky
1Institute of Neuroscience, University of Oregon, Eugene, OR 97403
3Department of Biology, University of Oregon, Eugene, OR 97403
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  • ORCID record for Johanna Tomorsky
Leah DeBlander
1Institute of Neuroscience, University of Oregon, Eugene, OR 97403
3Department of Biology, University of Oregon, Eugene, OR 97403
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Clifford G. Kentros
1Institute of Neuroscience, University of Oregon, Eugene, OR 97403
3Department of Biology, University of Oregon, Eugene, OR 97403
4Howard Hughes Medical Institute, University of Oregon, Eugene, OR 97403
5Kavli Institute of Systems Neuroscience, NTNU, Trondheim 7030, Norway
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Chris Q. Doe
1Institute of Neuroscience, University of Oregon, Eugene, OR 97403
2Institute of Molecular Biology, University of Oregon, Eugene, OR 97403
3Department of Biology, University of Oregon, Eugene, OR 97403
4Howard Hughes Medical Institute, University of Oregon, Eugene, OR 97403
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Cristopher M. Niell
1Institute of Neuroscience, University of Oregon, Eugene, OR 97403
3Department of Biology, University of Oregon, Eugene, OR 97403
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Article Information

DOI 
https://doi.org/10.1523/ENEURO.0181-17.2017
PubMed 
29085897
Published By 
Society for Neuroscience
History 
  • Received May 19, 2017
  • Revision received September 7, 2017
  • Accepted September 9, 2017
  • Published online September 19, 2017.
Copyright & Usage 
Copyright © 2017 Tomorsky et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Johanna Tomorsky1,3,
  2. Leah DeBlander1,3,
  3. Clifford G. Kentros1,3,4,5,
  4. Chris Q. Doe1,2,3,4 and
  5. Cristopher M. Niell1,3
  1. 1Institute of Neuroscience, University of Oregon, Eugene, OR 97403
  2. 2Institute of Molecular Biology, University of Oregon, Eugene, OR 97403
  3. 3Department of Biology, University of Oregon, Eugene, OR 97403
  4. 4Howard Hughes Medical Institute, University of Oregon, Eugene, OR 97403
  5. 5Kavli Institute of Systems Neuroscience, NTNU, Trondheim 7030, Norway
  1. Correspondence should be addressed to either of the following: Cristopher M. Niell, Institute of Neuroscience, Department of Biology, 1254 University of Oregon, Eugene, OR 97403, E-mail: cniell{at}uoregon.edu; or Chris Q. Doe, Institute of Neuroscience, Department of Biology, 1254 University of Oregon, Eugene, OR 97403, E-mail: cdoe{at}uoregon.edu.
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Author contributions

  1. Author contributions: J.T., C.M.N., C.Q.D., L.D., and C.G.K. designed research; J.T. and L.D. performed research; J.T. analyzed data; J.T., C.M.N., and C.Q.D. wrote the paper.

Disclosures

  • The authors declare no competing financial interests.

  • This work was supported by National Institutes of Health Grants T32-HD007348 and DP2-EY023190 and by the Howard Hughes Medical Institute, where C.Q.D. is an Investigator.

Funding

  • HHS | NIH | National Eye Institute (NEI)

    DP2-EY02190
  • HHS | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)

    T32-HD007348
  • Howard Hughes Medical Institute (HHMI)

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  • You are viewing the most recent version of this article.
  • previous version (September 19, 2017).

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Mar 202042722
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Apr 20211775
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Jun 202125121
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Jul 2025105321
Aug 202566213
Sep 2025710815
Oct 2025177239
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Total 202575780198
Total154777491991
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TU-Tagging: A Method for Identifying Layer-Enriched Neuronal Genes in Developing Mouse Visual Cortex
Johanna Tomorsky, Leah DeBlander, Clifford G. Kentros, Chris Q. Doe, Cristopher M. Niell
eNeuro 19 September 2017, 4 (5) ENEURO.0181-17.2017; DOI: 10.1523/ENEURO.0181-17.2017

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TU-Tagging: A Method for Identifying Layer-Enriched Neuronal Genes in Developing Mouse Visual Cortex
Johanna Tomorsky, Leah DeBlander, Clifford G. Kentros, Chris Q. Doe, Cristopher M. Niell
eNeuro 19 September 2017, 4 (5) ENEURO.0181-17.2017; DOI: 10.1523/ENEURO.0181-17.2017
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  • Article
    • Abstract
    • Significance Statement
    • Introduction
    • Materials and Methods
    • Immunohistochemistry
    • Sequence processing and differential expression analysis
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Keywords

  • gene expression
  • Layer 2/3
  • postnatal
  • transcriptomics
  • TU-tagging
  • visual cortex

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