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Research ArticleMethods/New Tools, Novel Tools and Methods

ABLE: An Activity-Based Level Set Segmentation Algorithm for Two-Photon Calcium Imaging Data

Stephanie Reynolds, Therese Abrahamsson, Renaud Schuck, P. Jesper Sjöström, Simon R. Schultz and Pier Luigi Dragotti
eNeuro 16 October 2017, 4 (5) ENEURO.0012-17.2017; https://doi.org/10.1523/ENEURO.0012-17.2017
Stephanie Reynolds
1Department of Electrical and Electronic Engineering, Imperial College London, South Kensington Campus, London SW7 2AZ, United Kingdom
2Centre for Neurotechnology, Imperial College London, South Kensington Campus, London SW7 2AZ, United Kingdom
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Therese Abrahamsson
3Centre for Research in Neuroscience, Brain Repair and Integrative Neuroscience Program, Department of Neurology and Neurosurgery, The Research Institute of the McGill University Health Centre, Montréal General Hospital, Montréal, Québec H3G 1A4, Canada
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Renaud Schuck
2Centre for Neurotechnology, Imperial College London, South Kensington Campus, London SW7 2AZ, United Kingdom
4Department of Bioengineering, Imperial College London, South Kensington Campus, London SW7 2AZ, United Kingdom
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P. Jesper Sjöström
3Centre for Research in Neuroscience, Brain Repair and Integrative Neuroscience Program, Department of Neurology and Neurosurgery, The Research Institute of the McGill University Health Centre, Montréal General Hospital, Montréal, Québec H3G 1A4, Canada
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Simon R. Schultz
2Centre for Neurotechnology, Imperial College London, South Kensington Campus, London SW7 2AZ, United Kingdom
4Department of Bioengineering, Imperial College London, South Kensington Campus, London SW7 2AZ, United Kingdom
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Pier Luigi Dragotti
1Department of Electrical and Electronic Engineering, Imperial College London, South Kensington Campus, London SW7 2AZ, United Kingdom
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Article Information

DOI 
https://doi.org/10.1523/ENEURO.0012-17.2017
PubMed 
29085906
Published By 
Society for Neuroscience
History 
  • Received January 10, 2017
  • Revision received September 14, 2017
  • Accepted September 15, 2017
  • Published online October 16, 2017.
Copyright & Usage 
Copyright © 2017 Reynolds et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Stephanie Reynolds1,2,
  2. Therese Abrahamsson3,
  3. Renaud Schuck2,4,
  4. P. Jesper Sjöström3,
  5. Simon R. Schultz2,4 and
  6. Pier Luigi Dragotti1
  1. 1Department of Electrical and Electronic Engineering, Imperial College London, South Kensington Campus, London SW7 2AZ, United Kingdom
  2. 2Centre for Neurotechnology, Imperial College London, South Kensington Campus, London SW7 2AZ, United Kingdom
  3. 3Centre for Research in Neuroscience, Brain Repair and Integrative Neuroscience Program, Department of Neurology and Neurosurgery, The Research Institute of the McGill University Health Centre, Montréal General Hospital, Montréal, Québec H3G 1A4, Canada
  4. 4Department of Bioengineering, Imperial College London, South Kensington Campus, London SW7 2AZ, United Kingdom
  1. Correspondence should be addressed to either of the following: Pier Luigi Dragotti, Department of Electrical and Electronic Engineering, Imperial College London, South Kensington Campus, London SW7 2AZ, United Kingdom, E-mail: p.dragotti{at}imperial.ac.uk; or Simon R. Schultz, Department of Bioengineering, Imperial College London, South Kensington Campus, London SW7 2AZ, United Kingdom, E-mail: s.schultz{at}imperial.ac.uk.
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Author contributions

  1. Author contributions: S.R., P.L.D., and S.R.S. designed research; S.R., T.A., R.S., and P.J.S. performed research; S.R. contributed unpublished reagents/analytic tools; S.R. analyzed data; S.R., P.L.D., and S.R.S. wrote the paper.

Disclosures

  • The authors declare no competing financial interests.

  • This work was supported by the European Research Council Starting Investigator Award (Grant 277800; to P.L.D.); the Biotechnology and Biological Sciences Research Council Grant BB/K001817/1 (to S.R.S.), the EU Marie Curie FP7 Initial Training Network (Grant 289146; to S.R.S.); Canadian Institutes of Health Research (CIHR) New Investigator Award (Grant 288936; to P.J.S.), the Canada Foundation for Innovation Leaders Opportunity Fund (Grant 28331; to P.J.S.), the CIHR Operating Grant 126137 (to P.J.S.), and Natural Sciences and Engineering Research Council of Canada Discovery Grant 418546-2 (to P.J.S.).

Funding

  • EC | European Research Council (ERC)

    277800
  • Biotechnology and Biological Sciences Research Council (BBSRC)

    BB/K001817/1
  • EU Marie Curie FP7 Initial Training Network

    289146
  • CIHR New Investigator Award

    288936
  • CIHR Operating Grant

    126137
  • Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada (NSERC)

    418546-2

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  • You are viewing the most recent version of this article.
  • previous version (October 16, 2017).

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Oct 201753355173
Nov 2017373215152
Dec 201710439527
Total 20171010665352
Jan 2018928724
Feb 20187311033
Mar 2018458436
Apr 20184710949
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Jun 2018697956
Jul 2018407917
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Jul 202138123
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Oct 202598225
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Total 2025103908273
Jan 20261612030
Feb 202631575
Total 20261927735
Total200982382137
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ABLE: An Activity-Based Level Set Segmentation Algorithm for Two-Photon Calcium Imaging Data
Stephanie Reynolds, Therese Abrahamsson, Renaud Schuck, P. Jesper Sjöström, Simon R. Schultz, Pier Luigi Dragotti
eNeuro 16 October 2017, 4 (5) ENEURO.0012-17.2017; DOI: 10.1523/ENEURO.0012-17.2017

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ABLE: An Activity-Based Level Set Segmentation Algorithm for Two-Photon Calcium Imaging Data
Stephanie Reynolds, Therese Abrahamsson, Renaud Schuck, P. Jesper Sjöström, Simon R. Schultz, Pier Luigi Dragotti
eNeuro 16 October 2017, 4 (5) ENEURO.0012-17.2017; DOI: 10.1523/ENEURO.0012-17.2017
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Keywords

  • Active Contour
  • calcium imaging
  • fluorescence microscopy
  • Level Set Method
  • segmentation

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