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Research ArticleNew Research, Neuronal Excitability

Acute Effect of Pore-Forming Clostridium perfringens ε-Toxin on Compound Action Potentials of Optic Nerve of Mouse

Mercè Cases, Artur Llobet, Beatrice Terni, Inmaculada Gómez de Aranda, Marta Blanch, Briain Doohan, Alexander Revill, Angus M. Brown, Juan Blasi and Carles Solsona
eNeuro 31 July 2017, 4 (4) ENEURO.0051-17.2017; https://doi.org/10.1523/ENEURO.0051-17.2017
Mercè Cases
1Laboratory of Cellular and Molecular Neurobiology, Department of Pathology and Experimental Therapeutics, Faculty of Medicine and Health Sciences, Campus of Bellvitge, University of Barcelona, Hospitalet de Llobregat, Barcelona 08907, Spain
2Institut d’Investigació Biomèdica de Bellvitge - Biomedical Research Institute of Bellvitge (IDIBELL), Hospitalet de Llobregat, Barcelona 08907, Spain
3Institute of Neurosciences, University of Barcelona, Barcelona 08035, Spain
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Artur Llobet
1Laboratory of Cellular and Molecular Neurobiology, Department of Pathology and Experimental Therapeutics, Faculty of Medicine and Health Sciences, Campus of Bellvitge, University of Barcelona, Hospitalet de Llobregat, Barcelona 08907, Spain
2Institut d’Investigació Biomèdica de Bellvitge - Biomedical Research Institute of Bellvitge (IDIBELL), Hospitalet de Llobregat, Barcelona 08907, Spain
3Institute of Neurosciences, University of Barcelona, Barcelona 08035, Spain
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Beatrice Terni
2Institut d’Investigació Biomèdica de Bellvitge - Biomedical Research Institute of Bellvitge (IDIBELL), Hospitalet de Llobregat, Barcelona 08907, Spain
3Institute of Neurosciences, University of Barcelona, Barcelona 08035, Spain
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Inmaculada Gómez de Aranda
1Laboratory of Cellular and Molecular Neurobiology, Department of Pathology and Experimental Therapeutics, Faculty of Medicine and Health Sciences, Campus of Bellvitge, University of Barcelona, Hospitalet de Llobregat, Barcelona 08907, Spain
3Institute of Neurosciences, University of Barcelona, Barcelona 08035, Spain
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Marta Blanch
1Laboratory of Cellular and Molecular Neurobiology, Department of Pathology and Experimental Therapeutics, Faculty of Medicine and Health Sciences, Campus of Bellvitge, University of Barcelona, Hospitalet de Llobregat, Barcelona 08907, Spain
2Institut d’Investigació Biomèdica de Bellvitge - Biomedical Research Institute of Bellvitge (IDIBELL), Hospitalet de Llobregat, Barcelona 08907, Spain
3Institute of Neurosciences, University of Barcelona, Barcelona 08035, Spain
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Briain Doohan
4School of Biomedical Sciences, Queen’s Medical Centre, University of Nottingham, Nottingham NG7 2UH, United Kingdom
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Alexander Revill
4School of Biomedical Sciences, Queen’s Medical Centre, University of Nottingham, Nottingham NG7 2UH, United Kingdom
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Angus M. Brown
4School of Biomedical Sciences, Queen’s Medical Centre, University of Nottingham, Nottingham NG7 2UH, United Kingdom
5Department of Neurology, University of Washington, Seattle, WA 98195
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Juan Blasi
1Laboratory of Cellular and Molecular Neurobiology, Department of Pathology and Experimental Therapeutics, Faculty of Medicine and Health Sciences, Campus of Bellvitge, University of Barcelona, Hospitalet de Llobregat, Barcelona 08907, Spain
2Institut d’Investigació Biomèdica de Bellvitge - Biomedical Research Institute of Bellvitge (IDIBELL), Hospitalet de Llobregat, Barcelona 08907, Spain
3Institute of Neurosciences, University of Barcelona, Barcelona 08035, Spain
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Carles Solsona
1Laboratory of Cellular and Molecular Neurobiology, Department of Pathology and Experimental Therapeutics, Faculty of Medicine and Health Sciences, Campus of Bellvitge, University of Barcelona, Hospitalet de Llobregat, Barcelona 08907, Spain
2Institut d’Investigació Biomèdica de Bellvitge - Biomedical Research Institute of Bellvitge (IDIBELL), Hospitalet de Llobregat, Barcelona 08907, Spain
3Institute of Neurosciences, University of Barcelona, Barcelona 08035, Spain
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Article Information

DOI 
https://doi.org/10.1523/ENEURO.0051-17.2017
PubMed 
28798954
Published By 
Society for Neuroscience
History 
  • Received February 14, 2017
  • Revision received May 31, 2017
  • Accepted June 16, 2017
  • Published online July 31, 2017.
Copyright & Usage 
Copyright © 2017 Cases et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Mercè Cases1,2,3,
  2. Artur Llobet1,2,3,
  3. Beatrice Terni2,3,
  4. Inmaculada Gómez de Aranda1,3,
  5. Marta Blanch1,2,3,
  6. Briain Doohan4,
  7. Alexander Revill4,
  8. Angus M. Brown4,5,
  9. Juan Blasi1,2,3 and
  10. Carles Solsona1,2,3
  1. 1Laboratory of Cellular and Molecular Neurobiology, Department of Pathology and Experimental Therapeutics, Faculty of Medicine and Health Sciences, Campus of Bellvitge, University of Barcelona, Hospitalet de Llobregat, Barcelona 08907, Spain
  2. 2Institut d’Investigació Biomèdica de Bellvitge - Biomedical Research Institute of Bellvitge (IDIBELL), Hospitalet de Llobregat, Barcelona 08907, Spain
  3. 3Institute of Neurosciences, University of Barcelona, Barcelona 08035, Spain
  4. 4School of Biomedical Sciences, Queen’s Medical Centre, University of Nottingham, Nottingham NG7 2UH, United Kingdom
  5. 5Department of Neurology, University of Washington, Seattle, WA 98195
  1. Correspondence should be addressed to either of the following: Carles Solsona, Laboratory of Cellular and Molecular Neurobiology, Department of Pathology and Experimental Therapeutics, Faculty of Medicine and Health Sciences, Campus of Bellvitge, University of Barcelona, Hospitalet de Llobregat, Barcelona 08907, Spain, E-mail: solsona{at}ub.edu; or Juan Blasi, Laboratory of Cellular and Molecular Neurobiology, Department of Pathology and Experimental Therapeutics, Faculty of Medicine and Health Sciences, Campus of Bellvitge, University of Barcelona, Hospitalet de Llobregat, Barcelona 08907, Spain, E-mail: blasi{at}ub.edu.
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Author contributions

  1. Author contributions: C.S., J.B., and A.L. designed research; M.C., B.T., I.G.d.A., M.B., B.D., A.R., A.M.B., J.B., and C.S. performed research; M.C., A.L., C.S., B.D., A.R., A.M.B. analysed data; M.C., A.L., A.M.B., B.T., J.B., and C.S. wrote the paper.

Disclosures

  • The authors declare no competing financial interests.

  • This work was supported by the Ministerio de Economía y Competitividad-Spanish Government Grant SAF2014-56811-R (to J.B. and C.S.).

Funding

  • Ministerio de Economía y Competitividad (MINECO)

    SAF2014-56811-R

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Apr 2018155139
May 2018307129
Jun 2018216133
Jul 2018153916
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Sep 2018184014
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Feb 20196717
Mar 20196649
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Jun 201934211
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Total 201956472106
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Feb 202051814
Mar 20203257
May 202002313
Jun 20201720
Jul 20201166
Aug 202002610
Sep 202053422
Oct 202078114
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Total 202040357141
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Feb 202124813
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Apr 20212667
May 2021417011
Jun 202141539
Jul 20212376
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Jun 202566613
Jul 202586124
Aug 202534216
Sep 2025510714
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Dec 20252173
Total 202564748161
Total139972131394
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Acute Effect of Pore-Forming Clostridium perfringens ε-Toxin on Compound Action Potentials of Optic Nerve of Mouse
Mercè Cases, Artur Llobet, Beatrice Terni, Inmaculada Gómez de Aranda, Marta Blanch, Briain Doohan, Alexander Revill, Angus M. Brown, Juan Blasi, Carles Solsona
eNeuro 31 July 2017, 4 (4) ENEURO.0051-17.2017; DOI: 10.1523/ENEURO.0051-17.2017

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Acute Effect of Pore-Forming Clostridium perfringens ε-Toxin on Compound Action Potentials of Optic Nerve of Mouse
Mercè Cases, Artur Llobet, Beatrice Terni, Inmaculada Gómez de Aranda, Marta Blanch, Briain Doohan, Alexander Revill, Angus M. Brown, Juan Blasi, Carles Solsona
eNeuro 31 July 2017, 4 (4) ENEURO.0051-17.2017; DOI: 10.1523/ENEURO.0051-17.2017
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Keywords

  • action potential
  • atp release
  • clostridial toxin
  • electrophysiology
  • myelin
  • optic nerve

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