Skip to main content

Main menu

  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Current Issue
    • Issue Archive
    • Blog
    • Collections
    • Podcast
  • TOPICS
    • Cognition and Behavior
    • Development
    • Disorders of the Nervous System
    • History, Teaching and Public Awareness
    • Integrative Systems
    • Neuronal Excitability
    • Novel Tools and Methods
    • Sensory and Motor Systems
  • ALERTS
  • FOR AUTHORS
  • ABOUT
    • Overview
    • Editorial Board
    • For the Media
    • Privacy Policy
    • Contact Us
    • Feedback
  • SUBMIT

User menu

Search

  • Advanced search
eNeuro

eNeuro

Advanced Search

 

  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Current Issue
    • Issue Archive
    • Blog
    • Collections
    • Podcast
  • TOPICS
    • Cognition and Behavior
    • Development
    • Disorders of the Nervous System
    • History, Teaching and Public Awareness
    • Integrative Systems
    • Neuronal Excitability
    • Novel Tools and Methods
    • Sensory and Motor Systems
  • ALERTS
  • FOR AUTHORS
  • ABOUT
    • Overview
    • Editorial Board
    • For the Media
    • Privacy Policy
    • Contact Us
    • Feedback
  • SUBMIT
PreviousNext
Research ArticleNew Research, Sensory and Motor Systems

Skill Learning Modulates RNA Pol II Poising at Immediate Early Genes in the Adult Striatum

Pedro Galvão-Ferreira, Michal Lipinski, Fernando Santos, Angel Barco and Rui M. Costa
eNeuro 6 April 2017, 4 (2) ENEURO.0074-17.2017; DOI: https://doi.org/10.1523/ENEURO.0074-17.2017
Pedro Galvão-Ferreira
1Champalimaud Neuroscience Programme, Fundação Champalimaud, Lisbon, 1400-038 Portugal
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Michal Lipinski
2Instituto de Neurociencias, Universidad Miguel Hernández - Consejo Superior de Investigaciones Científicas, Alicante, 03550 Spain
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Fernando Santos
1Champalimaud Neuroscience Programme, Fundação Champalimaud, Lisbon, 1400-038 Portugal
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Fernando Santos
Angel Barco
2Instituto de Neurociencias, Universidad Miguel Hernández - Consejo Superior de Investigaciones Científicas, Alicante, 03550 Spain
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Rui M. Costa
1Champalimaud Neuroscience Programme, Fundação Champalimaud, Lisbon, 1400-038 Portugal
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Article Figures & Data

Figures

  • Figure 1.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 1.

    Animal performance during a fast lever-pressing task. After one session of continuous reinforcement with self-paced delivery of up to 30 food pellets (CRF30), animals (n = 7) were required to perform on a FR schedule, whereby eight lever presses resulted in delivery of a food pellet within a time contingency, which ranged from one-thousand to 1 s (FR8-1000s to FR8-1s). A, Scheme representing the behavioral setup and structure of the fast lever-pressing task, as well as the task acquisition as represented by the average number of lever presses for each day of training (F(9,52) = 22.59, p = 0.0009). B, Distance of all seven consecutive IPIs from the final covert target (F(2.155,12.93) = 4.638, p = 0.0283). C, Percentage of sequences containing the minimum frequency target of the last session (end-target: 7 IPIs < 1s, ∼8.0 Hz; F(12,91) = 2.765, p = 0.0030); mean ± SEM represented in all graphs.

  • Figure 2.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 2.

    RNA polymerase II RPB1 phosphorylation in the striatum of mice trained in a fast lever-pressing task. A, Immunoblot analysis of the total RPB1 CTD repeat YS2PTS5PS7, with indication of its hyperphosphorylated (II0) and hypophosphorylated (IIA) forms, Ser5P+-enriched RPB1 CTD and Ser2P+-enriched RPB1 CTD. B, Actin quantification across both phospho-isoforms. C, RNA polymerase II poising index (calculated as the quotient between the Ser5-P and Ser2-P RPB1 CTD phospho-isoforms) in the striatum of mice trained in the fast lever pressing. For both control and trained groups, n = 7; data as mean ± SEM; ***p < 0.005.

  • Figure 3.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 3.

    Enrichment of RNA polymerase II RPB1 CTD phosphorylation forms at IEGs in the striatum of mice trained in a fast lever-pressing task. A, Graphical representation (not to scale) of the relative position of primers used in ChIP-qPCR experiments (primers represented in orange). B, ChIP-qPCR analysis of total RPB1 CTD binding at Gapdh and Tubb5 (positive control targets) and Arc and Fos (IEGs); controls n = 5; trained n = 6. C, ChIP-qPCR % of input data as a ratio between the promoter and gene bodies of all genomic targets for the total RPB1 CTD repeat. Data as mean ± SEM.

  • Figure 4.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 4.

    Dynamics of Ser5P+- and Ser2P+-RPB1 CTD enrichment and resulting poising indices at IEGs in the striatum of mice trained in a fast lever-pressing task. ChIP-qPCR % of input data for Ser5P+-enriched RPB1 CTD and Ser2P+-enriched RPB1 CTD (A) and Ser5P/Ser2P RNA polymerase II RPB1 CTD ratios (poising indices) (B) at Gapdh and Tubb5 (positive control targets) and Arc and Fos (IEGs) in the striatum of mice trained in the fast lever-pressing task (controls n = 3; trained n = 4). Data as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.005; n.s., p > 0.05.

  • Figure 5.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 5.

    RPB1 CTD poising index at IEGs in the striatum and its correlation with learning of a fast lever-pressing task. ChIP-qPCR % of input data for pooled Ser5P/Ser2P RNA polymerase II RPB1 CTD ratios (poising indices) at the promoters and gene bodies of positive control targets (Gapdh and Tubb5) and IEGs (Arc and Fos) in the striatum of (A) control and performance control mice (performance control animals were killed after completion of FR8-50s, roughly corresponding to a halfway point in the training regime), as well as mice fully trained in the fast lever-pressing task (controls n = 4; performance controls n = 4; trained n = 3); (B) performance control and fully trained mice grouped as, respectively, far from target and close to target, according to the distance to target value (i.e., distance of all seven consecutive IPIs from the final covert target) each animal presented at completion of FR8-50s or FR8-1s schedules, with far from target animals displaying a distance to target value over 0.6 and close to target animals a distance to target value under 0.6; and (C) mice trained in the fast lever-pressing task grouped according to the number of lever presses performed in their final training session, with low pressing animals finishing with under 250 lever presses and high pressing animals with over 250 lever presses. Data as mean ± SEM; *p < 0.05; **p < 0.01; n.s., p > 0.05.

Back to top

In this issue

eneuro: 4 (2)
eNeuro
Vol. 4, Issue 2
March/April 2017
  • Table of Contents
  • Index by author
Email

Thank you for sharing this eNeuro article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Skill Learning Modulates RNA Pol II Poising at Immediate Early Genes in the Adult Striatum
(Your Name) has forwarded a page to you from eNeuro
(Your Name) thought you would be interested in this article in eNeuro.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Print
View Full Page PDF
Citation Tools
Skill Learning Modulates RNA Pol II Poising at Immediate Early Genes in the Adult Striatum
Pedro Galvão-Ferreira, Michal Lipinski, Fernando Santos, Angel Barco, Rui M. Costa
eNeuro 6 April 2017, 4 (2) ENEURO.0074-17.2017; DOI: 10.1523/ENEURO.0074-17.2017

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Respond to this article
Share
Skill Learning Modulates RNA Pol II Poising at Immediate Early Genes in the Adult Striatum
Pedro Galvão-Ferreira, Michal Lipinski, Fernando Santos, Angel Barco, Rui M. Costa
eNeuro 6 April 2017, 4 (2) ENEURO.0074-17.2017; DOI: 10.1523/ENEURO.0074-17.2017
del.icio.us logo Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Significance Statement
    • Introduction
    • Materials and Methods
    • Results
    • Discussion
    • Acknowledgments
    • Footnotes
    • References
    • Synthesis
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Keywords

  • learning
  • Motor Skill
  • RNA Pol II
  • RPB1
  • striatum

Responses to this article

Respond to this article

Jump to comment:

No eLetters have been published for this article.

Related Articles

Cited By...

More in this TOC Section

New Research

  • Auditory cortex neurons show task-related and learning-dependent selectivity toward sensory input and reward during the learning process of an associative memory task
  • Responses to song playback differ in sleeping versus anesthetized songbirds
  • Hypothalamic TRH mediates anorectic effects of serotonin in rats
Show more New Research

Sensory and Motor Systems

  • Auditory cortex neurons show task-related and learning-dependent selectivity toward sensory input and reward during the learning process of an associative memory task
  • Responses to song playback differ in sleeping versus anesthetized songbirds
  • Hypothalamic TRH mediates anorectic effects of serotonin in rats
Show more Sensory and Motor Systems

Subjects

  • Sensory and Motor Systems

  • Home
  • Alerts
  • Visit Society for Neuroscience on Facebook
  • Follow Society for Neuroscience on Twitter
  • Follow Society for Neuroscience on LinkedIn
  • Visit Society for Neuroscience on Youtube
  • Follow our RSS feeds

Content

  • Early Release
  • Current Issue
  • Latest Articles
  • Issue Archive
  • Blog
  • Browse by Topic

Information

  • For Authors
  • For the Media

About

  • About the Journal
  • Editorial Board
  • Privacy Policy
  • Contact
  • Feedback
(eNeuro logo)
(SfN logo)

Copyright © 2022 by the Society for Neuroscience.
eNeuro eISSN: 2373-2822

The ideas and opinions expressed in eNeuro do not necessarily reflect those of SfN or the eNeuro Editorial Board. Publication of an advertisement or other product mention in eNeuro should not be construed as an endorsement of the manufacturer’s claims. SfN does not assume any responsibility for any injury and/or damage to persons or property arising from or related to any use of any material contained in eNeuro.