Research ArticleNew Research, Disorders of the Nervous System

Overexpression of Brain-Derived Neurotrophic Factor Protects Large Retinal Ganglion Cells After Optic Nerve Crush in Mice

Liang Feng, Zhen Puyang, Hui Chen, Peiji Liang, John B. Troy and Xiaorong Liu
eNeuro 5 January 2017, 4 (1) ENEURO.0331-16.2016; https://doi.org/10.1523/ENEURO.0331-16.2016

Published eLetters

Guidelines

As a forum for professional feedback, submissions of letters are open to all. You do not need to be a subscriber. To avoid redundancy, we urge you to read other people's letters before submitting your own. Name, current appointment, place of work, and email address are required to send a letter, and will be published with your review. We also require that you declare any competing financial interests. Unprofessional submissions will not be considered or responded to.

Submit a Response to This Article
Compose eLetter

More information about text formats

Plain text

  • No HTML tags allowed.
  • Web page addresses and e-mail addresses turn into links automatically.
  • Lines and paragraphs break automatically.
Author Information
First or given name, e.g. 'Peter'.
Your last, or family, name, e.g. 'MacMoody'.
Your email address, e.g. higgs-boson@gmail.com
Your role and/or occupation, e.g. 'Orthopedic Surgeon'.
Your organization or institution (if applicable), e.g. 'Royal Free Hospital'.
Statement of Competing Interests
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
14 + 1 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.

Vertical Tabs

Jump to comment:

  • Submitted on: (9 January 2017)
    Page navigation anchor for BDNF detection using Western blot
    BDNF detection using Western blot

    While I find this report to be of interest I am unsure about the data related to the quantification of BDNF by Western blot. Given its very low abundance, the detection and quantification of BDNF is notoriously difficult and its is unclear if BDNF can be reliably detected at all in the mouse retina by WB. Like most commercially available BDNF antibodies, AB1779SP used here has not been thoroughly validated and the apparent molecular weight illustrated in the corresponding data sheet is not as expected. Are BDNF mRNA data available to better appreciate the degree of over-expression of BDNF, even if BDNF protein data would be more relevant in principle ?
    Thank you.
    Y.-A. Barde

    Competing Interests: None declared.
Back to top

In this issue

eneuro: 4 (1)
eNeuro
Vol. 4, Issue 1
January/February 2017
Email
Print
View Full Page PDF
Citation Tools
Respond to this article
Overexpression of Brain-Derived Neurotrophic Factor Protects Large Retinal Ganglion Cells After Optic Nerve Crush in Mice
Liang Feng, Zhen Puyang, Hui Chen, Peiji Liang, John B. Troy, Xiaorong Liu
eNeuro 5 January 2017, 4 (1) ENEURO.0331-16.2016; DOI: 10.1523/ENEURO.0331-16.2016
Twitter logo Facebook logo Mendeley logo

Jump to section

Keywords

Responses to this article

Respond to this article

Jump to comment:

  • Published on: (9 January 2017)
    Page navigation anchor for BDNF detection using Western blot
    BDNF detection using Western blot

    While I find this report to be of interest I am unsure about the data related to the quantification of BDNF by Western blot. Given its very low abundance, the detection and quantification of BDNF is notoriously difficult and its is unclear if BDNF can be reliably detected at all in the mouse retina by WB. Like most commercially available BDNF antibodies, AB1779SP used here has not been thoroughly validated and the apparent molecular weight illustrated in the corresponding data sheet is not as expected. Are BDNF mRNA data available to better appreciate the degree of over-expression of BDNF, even if BDNF protein data would be more relevant in principle ?
    Thank you.
    Y.-A. Barde

    Competing Interests: None declared.

Subjects