Research ArticleNew Research, Disorders of the Nervous System

Overexpression of Brain-Derived Neurotrophic Factor Protects Large Retinal Ganglion Cells After Optic Nerve Crush in Mice

Liang Feng, Zhen Puyang, Hui Chen, Peiji Liang, John B. Troy and Xiaorong Liu
eNeuro 5 January 2017, 4 (1) ENEURO.0331-16.2016; DOI: https://doi.org/10.1523/ENEURO.0331-16.2016

Article Information

DOI 
https://doi.org/10.1523/ENEURO.0331-16.2016
PubMed 
28101532
Published By 
Society for Neuroscience
History 
  • Received November 3, 2016
  • Revision received December 21, 2016
  • Accepted December 28, 2016
  • Published online January 5, 2017.
Copyright & Usage 
Copyright © 2017 Feng et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Liang Feng1,2,
  2. Zhen Puyang1,3,4,
  3. Hui Chen1,2,
  4. Peiji Liang4,
  5. John B. Troy3 and
  6. Xiaorong Liu1,2
  1. 1Department of Ophthalmology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
  2. 2Department of Neurobiology, Weinberg College of Arts and Sciences, Northwestern University, Evanston, IL 60208, USA
  3. 3Department of Biomedical Engineering, Robert R. McCormick School of Engineering and Applied Science, Northwestern University, Evanston, IL 60208, USA
  4. 4School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China
  1. Correspondence should be addressed to Xiaorong Liu, Hogan 2-160, 2205 Tech Drive, Northwestern University, Evanston, IL 60208. E-mail: xiaorong-liu{at}northwestern.edu.
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Author contributions

  1. Author contributions: L.F., Z.P., and X.L. conceived and designed the experiments; P.L., J.B.T., and X.L. provided materials; L.F., Z.P., and H.C. performed the experiments and data analysis; L.F., Z.P., and X.L. prepared the Figures; L.F., Z.P., and X.L. wrote the manuscript, L.F., Z.P., H.C., P.L., J.B.T., and X.L. edited and approved the manuscript.

  2. L.F. and Z.P. contributed equally to this work.

Disclosures

  • The authors declare no competing financial interests.

  • This work was supported by the National Institutes of Health (NIH) grant R01EY026286 (X.L.) and grants from the BrightFocus Foundation (L.F. and X.L.), Northwestern Memorial Foundation (X.L.), Research to Prevent Blindness (Department of Ophthalmology), National Natural Science Foundation of China (P.L., NSFC No. 31471054), and the Program of Introducing Talents of Discipline to Universities (P.L., No. B08020).

Funding

  • HHS | NIH | National Eye Institute (NEI)

    100000053; R01EY026286

  • BrightFocus Foundation

    100006312

  • Research to Prevent Blindness (RPB)

    100001818

  • National Natural Science Foundation of China (NSFC)

    501100001809; 31471054

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eneuro: 4 (1)
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Vol. 4, Issue 1
January/February 2017
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Overexpression of Brain-Derived Neurotrophic Factor Protects Large Retinal Ganglion Cells After Optic Nerve Crush in Mice
Liang Feng, Zhen Puyang, Hui Chen, Peiji Liang, John B. Troy, Xiaorong Liu
eNeuro 5 January 2017, 4 (1) ENEURO.0331-16.2016; DOI: 10.1523/ENEURO.0331-16.2016
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  • Published on: (9 January 2017)
    Page navigation anchor for BDNF detection using Western blot
    BDNF detection using Western blot

    While I find this report to be of interest I am unsure about the data related to the quantification of BDNF by Western blot. Given its very low abundance, the detection and quantification of BDNF is notoriously difficult and its is unclear if BDNF can be reliably detected at all in the mouse retina by WB. Like most commercially available BDNF antibodies, AB1779SP used here has not been thoroughly validated and the apparent molecular weight illustrated in the corresponding data sheet is not as expected. Are BDNF mRNA data available to better appreciate the degree of over-expression of BDNF, even if BDNF protein data would be more relevant in principle ?
    Thank you.
    Y.-A. Barde

    Competing Interests: None declared.

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