Figure 7. mTOR signaling in DRG neurons of mTOR, Raptor, and Rictor KO mice. A, Sections of DRGs from WT, mTOR, Raptor, and Rictor KO mice, with p-mTOR (red) and Tuj1 (green) staining. Scale bar, 100 μm. B, Sections of DRGs from WT, mTOR, Raptor, and Rictor KO mice with or without CL, with p-mTOR (red) and Tuj1 (green) staining. p-S6 signal was eliminated in mTOR or Raptor KO mice. Scale bar, 100 μm. C, Sections of DRGs from WT and Raptor KO mice with or without CL, with p-4EBP1 (red) and Tuj1 (green) staining. Scale bar, 100 μm. D, Quantification of percentages of p-4EBP1+ DRG neurons in three groups of mice. One-way ANOVA followed by Tukey’s test. *p < 0.05, three mice in each group. E, Sections of DRGs from WT, mTOR, Raptor, and Rictor KO mice, with p-AKT473 (red) and Tuj1 (green) staining. Scale bar, 100 μm.