Interactions between Dorsal and Ventral Root Stimulation on the Generation of Locomotor-Like Activity in the Neonatal Mouse Spinal Cord

Stimulation of the DRG does not evoke locomotor-like activity if the corresponding DR is cut. A, Schematic to illustrate two possible ways in which DRG afferents (blue) might enter a VR. B, Stimulation of the DRG with the VR cut evokes locomotor-like activity. C, DRG stimulation fails to evoke locomotor-like activity when the DRG is stimulated with the DR cut. D, When the spinal nerve is stimulated with the DR cut, locomotor-like activity is evoked. E, DC recordings of the VRs during an episode of locomotor-like activity triggered by stimulation of the right DRG. F, When the corresponding DR is cut all VR activity is abolished when the DRG is stimulated.

The strength of locomotor-like activity increases with increasing stimulus intensity applied to either a dorsal or a ventral root. A, Locomotor-like activity elicited by stimulation of the L6 ventral root at 1× (top traces) and 1.1× (bottom traces) threshold intensity. B, In the same preparation, locomotor-like activity was elicited by stimulation of the L6 dorsal root at 1× (top traces) and 1.1× (bottom traces) threshold intensity. The bar indicates the duration of stimulus train (10 s). The values of locomotor strength (S; see Materials and Methods) computed for each of the displayed episodes is shown to the top and right of the traces. The displayed values reflect the effect of stimulation intensity. C, Locomotor strengths calculated for dorsal (558 from 63 experiments) and VR-evoked episodes (188 from 24 experiments) are plotted as a function of stimulus intensity expressed in multiples of threshold. The horizontal lines show median values and the ends of the vertical lines mark the 25th and 75th quartile of the sample set. The asterisk above a group indicates a statistically significant difference with the group immediately to the left.

Comparison of the locomotor-like activity produced by dorsal lumbar/sacral root (A) and lumbar ventral (B) root stimulation. The average coherent power (+SD) between the different root pairs is expressed with reference to the normalized power of the ipsilateral flexor and extensor VRs. For the DR stimulation episodes 300 trials from 45 preparations were used; for VR stimulation 145 trials from 25 preparations were used. The open circles represent the averaged value of the coherent power for each preparation (45 DR, 25 VR). The lines above the graph indicate significant differences between the connected pairs (Kruskal–Wallis followed by χ-squared statistic, p < 0.01). The grey lines highlight the significant differences between dorsal and ventral root stimulation. i F-E, L1 versus L5 Ipsilateral to the stimulated root; Bi F-F, left L1 versus right L1; c F-E, L1 versus L5 contralateral to the stimulated root; Bi E-E, left L5 versus right L5.

Stimulation of a dorsal, but not a ventral root evokes a short latency potential in the ventral funiculus. A, Schematic of the spinal cord showing the stimulation and recording setup. A stimulating electrode is applied to the left L6 DR (blue) and recording electrodes are applied to the ipsilateral L6 VR (red) and a strip of the contralateral L3 VF (green). Below the schematic are shown 25 superimposed recordings from the VF (green) and the VR (red) in response to DR stimulation (4× Thr) once every 30 s. The arrowheads show the point at which the minimum and maximum latency difference (jitter) was computed. B, The left pair of bars show the mean (±SD) onset latencies of the DR-evoked VR (red) and VF (green) responses, whereas the right pair show the average jitter for each type of recording (6 experiments; 25 trials each). *Indicates statistically significant difference in onset latency (p < 0.001, Tukey's multiple-comparisons test). C, Schematic showing the spinal cord with the left L6 VR (red) stimulated and the contralateral L3 VF (green) recorded. The dashed red line within the L6 segment shows the putative monosynaptic connection between the stimulated VR (red) and the cell bodies of the recorded VF interneurons (green). Below this is the response in the VF recording to 100 stimuli (100 µA) applied to the L6 VR every 30 s. The green area defines ±2 SD of the mean. D, Long-latency responses evoked in the VF in response to VR stimuli. E, Simultaneous low-pass filtered VR and VF recordings of a locomotor-episode in response to a VR stimulus train (4 Hz).

Simultaneous stimulation of dorsal and ventral roots at just subthreshold intensities does not elicit locomotor-like activity. A, B, Top traces show examples of locomotor-like rhythms elicited by stimulation of the homonymous dorsal (left, DR stim) and ventral (right, VR stim) roots at threshold intensity (1× Thr). The line at the bottom of the traces shows the duration of the stimulus train (10 s). The activity was high-pass filtered at 50 Hz. The traces below this show that locomotor-like activity was not produced by root when the stimulus intensity was set to 0.9× threshold. C, When the two subthreshold stimuli were combined locomotor activity was still not evoked. D, Box plots showing pooled data from several experiments (43 trials from 18 preparations). Stimulation of the dorsal and ventral alone or together does not result in locomotor-like activity. The values of strength were normalized to the mean of the ventral root sample set. The lines in the box plots show the median values, and the edges of the black rectangles show the 25th and 75th quartiles of the data.

Interleaved, suboptimal dorsal and ventral root-evoked responses combine to trigger locomotor-like activity. A, An episode of locomotor-like activity elicited by stimulation of the left L6 dorsal root with a train of electrical pulses appearing every 375 ms. The black traces show ventral root signals from the left (top) and right (bottom) L1 VRs, whereas the spectrogram above displays the corresponding cross-wavelet power spectrum. The hot regions in the spectrum with leftward pointing arrows indicate locomotor-like activity, while the cooler regions with rightward pointing arrows result from stimulus-locked synchronous responses. The vertical ticks below the VR traces indicate the stimulus pulses. The trace on the right shows the global power spectrum obtained by summing values in the cross-wavelet spectrum along the time dimension for all frequencies. The global power spectrum displayed here has been normalized by the maximum value. The number displayed next to the peak of the global power spectrum (red) indicates the frequency at which the power peaks. The red polar plot in the bottom right corner shows the power-weighted distribution of phase angles (arrows) for all regions of significant power appearing the cross-wavelet spectrogram. B, An episode of locomotor-like activity elicited in the same preparation as in A, but by stimulation of the L6 VR with a train of electrical pulses delivered at intervals of 375 ms (vertical red ticks). C, D, Activity resulting from the stimulation of the aforementioned dorsal and ventral root, respectively, but with pulse trains with an interpulse interval twice as long as in A and B (ie, 750 ms). The activity evoked at this stimulus frequency is not locomotor-like, although the bursts in the two VRs appear somewhat rhythmic, their phases are not tightly coupled and are completely out-of-phase. E, When the suboptimal dorsal and ventral root stimulus trains were offset by 375 ms so as to interleave the pulses delivered to the different roots (red and blue vertical ticks below the VR traces) and create a combined train with an effective interpulse interval of 375 ms, locomotor-like activity was evoked.

Interleaved, suboptimal brainstem and ventral root stimulus trains combine to trigger locomotor-like activity. The layout of this figure is the same as that of Figure7. A, B, Cross-wavelet spectrograms (top) of locomotor-like activity recorded from the left and right L6 VRs (black traces, bottom) evoked in response to stimulation of the brainstem or the L5 VR with an optimal 6.7 Hz stimulus train. The blue and red bars below the VR activity represent the stimuli. The maximum XW power is at 0.6 Hz (brainstem) and 0.77 Hz (VR) and is shown in the power spectra on the right. The activity between the roots is alternating (arrows to the left, phase plot in the bottom right corner). C, D, Stimulation of the brainstem at the suboptimal frequency of 3.33 Hz evoked synchronized bursting (arrows to the right). Stimulation of the VR at the 3.33 Hz evoked weak alternating bursts together with the stimulus locked synchronous signals. E, Interleaving the 3.33 Hz brainstem and VR stimulus trains (alternating blue and red bars) evoked locomotor-like activity.

Comparison of the strength of locomotor-like activity evoked by optimal, suboptimal, and interleaved dorsal and ventral root stimulation. Box and whisker plots compare the strengths of locomotor-like episodes evoked when stimulating at the optimal frequency, suboptimal frequency and interleaved suboptimal frequencies. The horizontal lines within the boxes indicate the median values, the lower and upper edges of the boxes mark the 25th and 75th percentile, and the whiskers extend to the most extreme data points. The X indicates that locomotor-like activity could not be elicited. The strength is expressed as normalized values because data from different preparations were pooled. The data were generated from seven ipsilateral pairs of roots and six contralateral pairs.