Article Figures & Data
Figures
- Figure 1.
Experimental Timeline and MOs Projections. A, Experimental timeline. Between P25 and P30, mice were allowed to sleep normally (C) or subjected to CSR. All mice were injected with AAV-GFP between P44 and P47, and each mouse was perfused exactly 3 weeks later. Middle, Right, The location of the viral injections on the skull and in a coronal brain section. A, Anterior; P, posterior; D, dorsal; V ventral; B, bregma. B, Example of projections from MOs in two representative mice (C and CSR) 3 weeks after injection of AAV-GFP. Measurements are given in millimeters from bregma. Final analysis included 14 mice in each group.
- Figure 2.
Projection Fractions in Controls and CSR Mice. A, A plot of projection fractions (normalized by injection volume) across all mice (y-axis) and all regions (x-axis). B, Correlations of projection fraction patterns in mice with injection site. The mouse with the most extreme distance in injection location is taken as the basis for correlation (and is by definition equal to one). All regions were first normalized to have unit means to account for differences between injections. Without this normalization, the mean pairwise correlation of all animals is 0.903 ± 0.07. Distance in micrometers is measured to the center of the injection site from the midline (826 ± 140, left), the midline merging of the anterior commissure (1771 ± 262, middle) or the pial surface (636± 60, right). Values are given for Pearson’s rho. C, Left, Box plot of the condition influence,
, of the GLM for all regions. A positive
indicates that the control group has a higher connectivity than the CSR group (see Results for details). Right, An expanded version that is aligned and color-coded to match the subdivision in major anatomical regions.
Tables
Property Data Structure Type of test p value Fig 2B: medial–lateral effect on injections Normally distributed Student’s t test <0.0001 Fig 2B: anterior–posterior effect on injections Normally distributed Student’s t test 0.004 Fig 2B: dorsal–ventral effect on injections Normally distributed Student’s t test 0.796 Control/CSR influence on connectivity (
)Normality not assumed One-tailed bootstrap 0.252 Males: control/CSR influence on connectivity (
)Normality not assumed One-tailed bootstrap 0.491 Females: control/CSR influence on connectivity (
)Normality not assumed One-tailed bootstrap 0.258 Control/CSR influence on weight (control > CSR) Normally distributed Paired t test <0.0001 Control: male/female weight gain (male > female) Normally distributed Paired t test 0.007 CSR: male/female weight gain (male > female)Accuracy of classification algorithm Normally distributedBinomial distributed Paired t testBinomial cumulative distribution function test 0.0080.0005 Region no. Region abbreviation Region name Region category 1 FRP Frontal pole, cerebral cortex Isocortex 2 MOp Primary motor area Isocortex 3 MOs Secondary motor area Isocortex 4 SSp-n Primary somatosensory area, nose Isocortex 5 SSp-bfd Primary somatosensory area, barrel field Isocortex 6 SSp-ll Primary somatosensory area, lower limb Isocortex 7 SSp-m Primary somatosensory area, mouth Isocortex 8 SSp-ul Primary somatosensory area, upper limb Isocortex 9 SSp-tr Primary somatosensory area, trunk Isocortex 10 SSp-un Primary somatosensory area, unassigned Isocortex 11 SSs Supplemental somatosensory area Isocortex 12 GU Gustatory areas Isocortex 13 VISC Visceral area Isocortex 14 AUDd Dorsal auditory area Isocortex 15 AUDp Primary auditory area Isocortex 16 AUDpo Posterior auditory area Isocortex 17 AUDv Ventral auditory area Isocortex 18 VISal Anterolateral visual area Isocortex 19 VISam Anteromedial visual area Isocortex 20 VISl Lateral visual area Isocortex 21 VISp Primary visual area Isocortex 22 VISpl Posterolateral visual area Isocortex 23 VISpm Posteromedial visual area Isocortex 24 VISli Isocortex 25 VISpor Isocortex 26 ACAd Anterior cingulate area, dorsal part Isocortex 27 ACAv Anterior cingulate area, ventral part Isocortex 28 PL Prelimbic area Isocortex 29 ILA Infralimbic area Isocortex 30 ORBl Orbital area, lateral part Isocortex 31 ORBm Orbital area, medial part Isocortex 32 ORBvl Orbital area, ventrolateral part Isocortex 33 AId Agranular insular area, dorsal part Isocortex 34 AIp Agranular insular area, posterior part Isocortex 35 AIv Agranular insular area, ventral part Isocortex 36 RSPagl Retrosplenial area, lateral agranular part Isocortex 37 RSPd Retrosplenial area, dorsal part Isocortex 38 RSPv Retrosplenial area, ventral part Isocortex 39 VISa Isocortex 40 VISrl Isocortex 41 TEa Temporal association areas Isocortex 42 PERI Perirhinal area Isocortex 43 ECT Ectorhinal area Isocortex 45 AOB Accessory olfactory bulb Olfactory areas 46 AON Anterior olfactory nucleus Olfactory areas 47 TT Taenia tecta Olfactory areas 48 DP Dorsal peduncular area Olfactory areas 49 PIR Piriform area Olfactory areas 50 NLOT Nucleus of the lateral olfactory tract Olfactory areas 51 COAa Cortical amygdalar area, anterior part Olfactory areas 57 CA3 Field CA3 Hippocampal formation 59 FC Fasciola cinerea Hippocampal formation 60 IG Induseum griseum Hippocampal formation 61 ENTl Entorhinal area, lateral part Hippocampal formation 65 POST Postsubiculum Hippocampal formation 66 PRE Presubiculum Hippocampal formation 67 SUB Subiculum Hippocampal formation 68 CLA Claustrum Claustrum + amygdala 69 EPd Endopiriform nucleus, dorsal part Claustrum + amygdala 70 EPv Endopiriform nucleus, ventral part Claustrum + amygdala 71 LA Lateral amygdalar nucleus Claustrum + amygdala 72 BLA Basolateral amygdalar nucleus Claustrum + amygdala 73 BMA Basomedial amygdalar nucleus Claustrum + amygdala 74 PA Posterior amygdalar nucleus Claustrum + amygdala 75 CP Caudoputamen Striatum + pallidum 76 ACB Nucleus accumbens Striatum + pallidum 77 FS Fundus of striatum Striatum + pallidum 78 OT Olfactory tubercle Striatum + pallidum 79 LSc Lateral septal nucleus, caudal (caudodorsal) part Striatum + pallidum 80 LSr Lateral septal nucleus, rostral (rostroventral) part Striatum + pallidum 83 SH Septohippocampal nucleus Striatum + pallidum 84 AAA Anterior amygdalar area Striatum + pallidum 86 CEA Central amygdalar nucleus Striatum + pallidum 87 IA Intercalated amygdalar nucleus Striatum + pallidum 88 MEA Medial amygdalar nucleus Striatum + pallidum 89 GPe Globus pallidus, external segment Striatum + pallidum 90 GPi Globus pallidus, internal segment Striatum + pallidum 91 SI Substantia innominata Striatum + pallidum 92 MA Magnocellular nucleus Striatum + pallidum 94 NDB Diagonal band nucleus Striatum + pallidum 96 BST Bed nuclei of the stria terminalis Striatum + pallidum 97 BAC Bed nucleus of the anterior commissure Striatum + pallidum 98 VAL Ventral anterior-lateral complex of the thalamus Thalamus 99 VM Ventral medial nucleus of the thalamus Thalamus 100 VPL Ventral posterolateral nucleus of the thalamus Thalamus 101 VPLpc Ventral posterolateral nucleus of the thalamus, parvicellular part Thalamus 102 VPM Ventral posteromedial nucleus of the thalamus Thalamus 103 VPMpc Ventral posteromedial nucleus of the thalamus, parvicellular part Thalamus 104 SPFm Subparafascicular nucleus, magnocellular part Thalamus 105 SPFp Subparafascicular nucleus, parvicellular part Thalamus 106 SPA Subparafascicular area Thalamus 107 PP Peripeduncular nucleus Thalamus 108 MG Medial geniculate complex Thalamus 110 LP Lateral posterior nucleus of the thalamus Thalamus 111 PO Posterior complex of the thalamus Thalamus 112 POL Posterior limiting nucleus of the thalamus Thalamus 113 SGN Suprageniculate nucleus Thalamus 114 AV Anteroventral nucleus of thalamus Thalamus 115 AM Anteromedial nucleus Thalamus 116 AD Anterodorsal nucleus Thalamus 117 IAM Interanteromedial nucleus of the thalamus Thalamus 118 IAD Interanterodorsal nucleus of the thalamus Thalamus 119 LD Lateral dorsal nucleus of thalamus Thalamus 120 IMD Intermediodorsal nucleus of the thalamus Thalamus 121 MD Mediodorsal nucleus of thalamus Thalamus 122 SMT Submedial nucleus of the thalamus Thalamus 123 PR Perireunensis nucleus Thalamus 124 PVT Paraventricular nucleus of the thalamus Thalamus 125 PT Parataenial nucleus Thalamus 126 RE Nucleus of reunions Thalamus 127 RH Rhomboid nucleus Thalamus 128 CM Central medial nucleus of the thalamus Thalamus 129 PCN Paracentral nucleus Thalamus 130 CL Central lateral nucleus of the thalamus Thalamus 131 PF Parafascicular nucleus Thalamus 132 RT Reticular nucleus of the thalamus Thalamus 133 IGL Intergeniculate leaflet of the lateral geniculate complex Thalamus 134 LGv Ventral part of the lateral geniculate complex Thalamus 135 SubG Subgeniculate nucleus Thalamus 137 LH Lateral habenula Thalamus 138 SO Supraoptic nucleus Hypothalamus 140 PVH Paraventricular hypothalamic nucleus Hypothalamus 142 PVi Periventricular hypothalamic nucleus, intermediate part Hypothalamus 145 AVP Anteroventral preoptic nucleus Hypothalamus 147 DMH Dorsomedial nucleus of the hypothalamus Hypothalamus 158 VLPO Ventrolateral preoptic nucleus Hypothalamus 160 LM Lateral mammillary nucleus Hypothalamus 161 MM Medial mammillary nucleus Hypothalamus 162 SUM Supramammillary nucleus Hypothalamus 163 TMd Tuberomammillary nucleus, dorsal part Hypothalamus 164 TMv Tuberomammillary nucleus, ventral part Hypothalamus 166 PMd Dorsal premammillary nucleus Hypothalamus 168 PVHd Paraventricular hypothalamic nucleus, descending division Hypothalamus 170 PH Posterior hypothalamic nucleus Hypothalamus 171 LHA Lateral hypothalamic area Hypothalamus 172 LPO Lateral preoptic area Hypothalamus 173 PST Preparasubthalamic nucleus Hypothalamus 174 PSTN Parasubthalamic nucleus Hypothalamus 176 STN Subthalamic nucleus Hypothalamus 177 TU Tuberal nucleus Hypothalamus 178 ZI Zona incerta Hypothalamus 179 SCs Superior colliculus, sensory related Midbrain + pons 180 IC Inferior colliculus Midbrain + pons 181 NB Nucleus of the brachium of the inferior colliculus Midbrain + pons 182 SAG Nucleus sagulum Midbrain + pons 183 PBG Parabigeminal nucleus Midbrain + pons 184 MEV Midbrain trigeminal nucleus Midbrain + pons 185 SNr Substantia nigra, reticular part Midbrain + pons 186 VTA Ventral tegmental area Midbrain + pons 187 RR Midbrain reticular nucleus, retrorubral area Midbrain + pons 188 MRN Midbrain reticular nucleus Midbrain + pons 189 SCm Superior colliculus, motor related Midbrain + pons 190 PAG Periaqueductal gray Midbrain + pons 191 APN Anterior pretectal nucleus Midbrain + pons 192 MPT Medial pretectal area Midbrain + pons 193 NOT Nucleus of the optic tract Midbrain + pons 194 NPC Nucleus of the posterior commissure Midbrain + pons 195 OP Olivary pretectal nucleus Midbrain + pons 196 PPT Posterior pretectal nucleus Midbrain + pons 197 CUN Cuneiform nucleus Midbrain + pons 198 RN Red nucleus Midbrain + pons 199 III Oculomotor nucleus Midbrain + pons 200 EW Edinger–Westphal nucleus Midbrain + pons 201 IV Trochlear nucleus Midbrain + pons 202 VTN Ventral tegmental nucleus Midbrain + pons 203 AT Anterior tegmental nucleus Midbrain + pons 204 LT Lateral terminal nucleus of the accessory optic tract Midbrain + pons 205 SNc Substantia nigra, compact part Midbrain + pons 206 PPN Pedunculopontine nucleus Midbrain + pons 207 IF Interfascicular nucleus raphe Midbrain + pons 208 IPN Interpeduncular nucleus Midbrain + pons 209 RL Rostral linear nucleus raphe Midbrain + pons 210 CLI Central linear nucleus raphe Midbrain + pons 211 DR Dorsal nucleus raphe Midbrain + pons 212 NLL Nucleus of the lateral lemniscus Midbrain + pons 213 PSV Principal sensory nucleus of the trigeminal Midbrain + pons 214 PB Parabrachial nucleus Midbrain + pons 215 SOC Superior olivary complex Midbrain + pons 216 B Barringtons nucleus Midbrain + pons 217 DTN Dorsal tegmental nucleus Midbrain + pons 218 PCG Pontine central gray Midbrain + pons 219 PG Pontine gray Midbrain + pons 220 PRNc Pontine reticular nucleus, caudal part Midbrain + pons 221 SG Supragenual nucleus Midbrain + pons 222 SUT Supratrigeminal nucleus Midbrain + pons 223 TRN Tegmental reticular nucleus Midbrain + pons 224 V Motor nucleus of trigeminal Midbrain + pons 225 CS Superior central nucleus raphe Midbrain + pons 226 LC Locus ceruleus Midbrain + pons 227 LDT Laterodorsal tegmental nucleus Midbrain + pons 228 NI Nucleus incertus Midbrain + pons 229 PRNr Pontine reticular nucleus Midbrain + pons 230 RPO Nucleus raphe pontis Midbrain + pons 231 SLC Subceruleus nucleus Midbrain + pons 232 SLD Sublaterodorsal nucleus Midbrain + pons 236 CU Cuneate nucleus Medulla 239 NTB Nucleus of the trapezoid body Medulla 240 NTS Nucleus of the solitary tract Medulla 241 SPVC Spinal nucleus of the trigeminal, caudal part Medulla 242 SPVI Spinal nucleus of the trigeminal, interpolar part Medulla 243 SPVO Spinal nucleus of the trigeminal, oral part Medulla 244 VI Abducens nucleus Medulla 245 VII Facial motor nucleus Medulla 246 ACVII Accessory facial motor nucleus Medulla 247 AMB Nucleus ambiguus Medulla 248 DMX Dorsal motor nucleus of the vagus nerve Medulla 249 GRN Gigantocellular reticular nucleus Medulla 250 ICB Infracerebellar nucleus Medulla 251 IO Inferior olivary complex Medulla 252 IRN Intermediate reticular nucleus Medulla 253 ISN Inferior salivatory nucleus Medulla 254 LIN Linear nucleus of the medulla Medulla 255 LRN Lateral reticular nucleus Medulla 256 MARN Magnocellular reticular nucleus Medulla 257 MDRNd Medullary reticular nucleus, dorsal part Medulla 258 MDRNv Medullary reticular nucleus, ventral part Medulla 259 PARN Parvicellular reticular nucleus Medulla 260 PAS Parasolitary nucleus Medulla 261 PGRNd Paragigantocellular reticular nucleus, dorsal part Medulla 262 PGRNl Paragigantocellular reticular nucleus, lateral part Medulla 263 NR Nucleus of Roller Medulla 264 PRP Nucleus prepositus Medulla 265 PPY Parapyramidal nucleus Medulla 266 LAV Lateral vestibular nucleus Medulla 267 MV Medial vestibular nucleus Medulla 268 SPIV Spinal vestibular nucleus Medulla 269 SUV Superior vestibular nucleus Medulla 270 x Nucleus x Medulla 271 XII Hypoglossal nucleus Medulla 272 y Nucleus y Medulla 273 RM Nucleus raphe magnus Medulla 274 RPA Nucleus raphe pallidus Medulla 275 RO Nucleus raphe obscurus Medulla 290 FN Fastigial nucleus Cerebellum 291 IP Interposed nucleus Cerebellum 292 DN Dentate nucleus Cerebellum 293 fiber tracts Fiber tracts Fiber tracts
In this issue
Effects of Chronic Sleep Restriction during Early Adolescence on the Adult Pattern of Connectivity of Mouse Secondary Motor Cortex
