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Research ArticleNew Research, Neuronal Excitability

Targeted Deletion of Vesicular GABA Transporter from Retinal Horizontal Cells Eliminates Feedback Modulation of Photoreceptor Calcium Channels

Arlene A. Hirano, Xue Liu, Jim Boulter, James Grove, Luis Pérez de Sevilla Müller, Steven Barnes and Nicholas C. Brecha
eNeuro 28 February 2016, 3 (1) ENEURO.0148-15.2016; https://doi.org/10.1523/ENEURO.0148-15.2016
Arlene A. Hirano
1Department of Neurobiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California 90095
2Veterans Administration Greater Los Angeles Healthcare System, Los Angeles, California 90073
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  • ORCID record for Arlene A. Hirano
Xue Liu
1Department of Neurobiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California 90095
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Jim Boulter
3Department of Psychiatry and Biobehavioral Sciences, Hatos Research Center for Neuropharmacology, Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California 90095
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James Grove
1Department of Neurobiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California 90095
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Luis Pérez de Sevilla Müller
1Department of Neurobiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California 90095
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Steven Barnes
1Department of Neurobiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California 90095
2Veterans Administration Greater Los Angeles Healthcare System, Los Angeles, California 90073
4Department of Physiology and Biophysics, Dalhousie University, Halifax, Nova Scotia B3H 4R2, Canada
5Department of Ophthalmology & Visual Sciences, Dalhousie University, Halifax, Nova Scotia B3H 4R2, Canada
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Nicholas C. Brecha
1Department of Neurobiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California 90095
2Veterans Administration Greater Los Angeles Healthcare System, Los Angeles, California 90073
6Jules Stein Eye Institute, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California 90095
7Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California 90095
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Article Information

DOI 
https://doi.org/10.1523/ENEURO.0148-15.2016
PubMed 
27022629
Published By 
Society for Neuroscience
History 
  • Received December 2, 2015
  • Revision received January 20, 2016
  • Accepted January 28, 2016
  • Published online February 28, 2016.
Copyright & Usage 
Copyright © 2016 Hirano et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Arlene A. Hirano1,2,
  2. Xue Liu1,
  3. Jim Boulter3,
  4. James Grove1,
  5. Luis Pérez de Sevilla Müller1,
  6. Steven Barnes1,2,4,5,* and
  7. Nicholas C. Brecha1,2,6,7,*
  1. 1Department of Neurobiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California 90095
  2. 2Veterans Administration Greater Los Angeles Healthcare System, Los Angeles, California 90073
  3. 3Department of Psychiatry and Biobehavioral Sciences, Hatos Research Center for Neuropharmacology, Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California 90095
  4. 4Department of Physiology and Biophysics, Dalhousie University, Halifax, Nova Scotia B3H 4R2, Canada
  5. 5Department of Ophthalmology & Visual Sciences, Dalhousie University, Halifax, Nova Scotia B3H 4R2, Canada
  6. 6Jules Stein Eye Institute, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California 90095
  7. 7Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California 90095
  1. Correspondence should be addressed to Arlene A. Hirano, Department of Neurobiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095. E-mail: ahirano{at}mednet.ucla.edu.
  • X. Liu’s present address: Biomaterials and Live Cell Imaging Institute, Chongqing University of Science and Technology, Chongqing 400044, People’s Republic of China.

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Author contributions

  1. 2 Author contributions: A.A.H., J.B., S.B., and N.C.B. designed research; A.A.H., X.L., J.B., J.G., L.P.d.S.M., and S.B. performed research; A.A.H., X.L., J.B., J.G., L.P.d.S.M., S.B., and N.C.B. analyzed data; A.A.H., S.B., and N.C.B. wrote the paper.

  • X. Liu’s present address: Biomaterials and Live Cell Imaging Institute, Chongqing University of Science and Technology, Chongqing 400044, People’s Republic of China.

Disclosures

  • 1 The authors declare no competing financial interests.

  • 3 This research was supported by National Institutes of Health Grant EY-15573 (N.C.B.), UCLA Oppenheimer Seed Grant (A.A.H., J.B., N.C.B.), the Plum Foundation (S.B., N.C.B.), a Veterans Administration Career Scientist Award (N.C.B.), Canadian Institutes of Health Research-Nova Scotia Health Research Foundation Regional Partnership Program Grant MOP10968 (S.B.), and Natural Sciences and Engineering Research Council of Canada Discovery Award 194640 (S.B.).

  • ↵* S.B. and N.C.B. are co-senior authors.

Funding

  • National Institutes of Health

    EY15573
  • UCLA Oppenheimer Seed Grant

  • UCLA Oppenheimer Seed Grant

  • UCLA Oppenheimer Seed Grant

  • Plum Foundation

  • Plum Foundation

  • Veterans Administration Career Scientist Award

  • Canadian Institutes of Health Research-Nova Scotia Health Research Foundation Regional Partnership Program

    MOP10968
  • Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery Award

    194640

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  • previous version (February 28, 2016).

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Targeted Deletion of Vesicular GABA Transporter from Retinal Horizontal Cells Eliminates Feedback Modulation of Photoreceptor Calcium Channels
Arlene A. Hirano, Xue Liu, Jim Boulter, James Grove, Luis Pérez de Sevilla Müller, Steven Barnes, Nicholas C. Brecha
eNeuro 28 February 2016, 3 (1) ENEURO.0148-15.2016; DOI: 10.1523/ENEURO.0148-15.2016

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Targeted Deletion of Vesicular GABA Transporter from Retinal Horizontal Cells Eliminates Feedback Modulation of Photoreceptor Calcium Channels
Arlene A. Hirano, Xue Liu, Jim Boulter, James Grove, Luis Pérez de Sevilla Müller, Steven Barnes, Nicholas C. Brecha
eNeuro 28 February 2016, 3 (1) ENEURO.0148-15.2016; DOI: 10.1523/ENEURO.0148-15.2016
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Keywords

  • Ca channels
  • Cx57-iCre
  • GABA receptors
  • inhibitory feedback
  • retinal horizontal cells
  • synaptic vesicles

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