Article Figures & Data
Figures
- Figure 1.
Western blot quantitative analysis of KLF4 protein expression in the retinae of KLF4 cKO and control mice. A, Representative images of Western blots against KLF4 and GAPDH as marked. B, Quantification of the relative protein expression normalized to total protein and GAPDH as marked (N = 3 for each biological sample; values are reported as the mean ± SD).
- Figure 2.
A, B, KLF4 is not required for photoreceptors or outer segments. Immunofluorescence for recoverin (A) and rhodopsin (B; green) was counterstained with DAPI for nuclei (blue) in retina cross sections from wild-type control mice (top) and KLF4-cKO mice (bottom). IPL, Inner plexiform layer. C, D, The thickness of recoverin+ (C) and rhodopsin+ (D) layers (as marked with brackets in A and B) were measured and found to be nonsignificant (n.s.; values are reported as the mean ± SD; N = 18). Scale bar, 50 μm.
- Figure 3.
KLF4 does not affect rod bipolar cell numbers. A, Retinal cross sections were immunostained for PKC-α (orange) and counterstained with DAPI (blue). B, PKC-α+ cells in the INL showed no statistically significant difference between WT and KLF4-cKO groups (n.s.; values are reported as the mean ± SD; N = 18). Scale bar, 50 μm.
- Figure 4.
KLF4 does not affect Müller glial cell numbers. A, Immunofluorescence staining was performed to detect GS (orange), and cells were counterstained with DAPI (blue). B, The GS+ cells in the INL demonstrated no statistical difference between WT and KLF4-cKO groups (n.s.; values are reported as the mean ± SD; N = 18). Scale bar, 50 μm.
- Figure 5.
KLF4 is not required for amacrine cell numbers. A, Immunofluorescence staining for Pax6 (orange) and counterstaining with DAPI (blue). B, Pax6+ cells per unit length showed no statistical difference between WT and KLF4-cKO groups in either the GCL or INL (n.s.; values are reported as the mean ± SD; N = 18). Scale bar, 50 μm.
- Figure 6.
KLF4 is not required for RGC cell numbers. A, Retinal cross sections were immunostained for Brn3a (orange) and counterstained with DAPI (blue). B, Brn3a+ cells showed no statistically significant difference between WT and KLF4-cKO groups (n.s.; values are reported as the mean ± SD; N = 18). Scale bar, 50 μm.
- Figure 7.
KLF4 deficiency leads to an increase in RGC axon fascicles in the adult retina. A, Representative immunofluorescent staining of retinal flat mount. Scale bar, 200 μm. B, Retinal cross sections were immunostained for β-tubulin (green) and counterstained with DAPI (blue). C, The thickness of RGC axon fascicles (brackets in B) was measured. There was a significant increase in nerve fiber layer axon bundle thickness in KLF4-cKO compared with WT mice (*p < 0.05; values are reported as the mean ± SD; N = 18). Scale bar, 50 μm.
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