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Research ArticleNew Research, Disorders of the Nervous System

Rapid Increases in proBDNF after Pilocarpine-Induced Status Epilepticus in Mice Are Associated with Reduced proBDNF Cleavage Machinery

Ajay X. Thomas, Yasmin Cruz Del Angel, Marco I. Gonzalez, Andrew J. Carrel, Jessica Carlsen, Philip M. Lam, Barbara L. Hempstead, Shelley. J. Russek and Amy R. Brooks-Kayal
eNeuro 9 February 2016, 3 (1) ENEURO.0020-15.2016; https://doi.org/10.1523/ENEURO.0020-15.2016
Ajay X. Thomas
1Department of Pediatrics, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045
2Neuroscience Program, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045
3Medical Scientist Training Program, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045
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Yasmin Cruz Del Angel
1Department of Pediatrics, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045
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Marco I. Gonzalez
1Department of Pediatrics, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045
2Neuroscience Program, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045
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Andrew J. Carrel
1Department of Pediatrics, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045
2Neuroscience Program, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045
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Jessica Carlsen
1Department of Pediatrics, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045
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Philip M. Lam
1Department of Pediatrics, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045
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Barbara L. Hempstead
4Departments of Medicine, Hematology & Medical Oncology, Weil Cornell Medical College, New York, New York 10045
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Shelley. J. Russek
5Department of Pharmacology, Boston University School of Medicine, Boston, Massachusetts 02118
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Amy R. Brooks-Kayal
1Department of Pediatrics, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045
2Neuroscience Program, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045
6Department of Neurology, Children’s Hospital Colorado, Aurora, Colorado 80045
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Article Information

DOI 
https://doi.org/10.1523/ENEURO.0020-15.2016
PubMed 
27057559
Published By 
Society for Neuroscience
History 
  • Received March 1, 2015
  • Revision received January 22, 2016
  • Accepted January 28, 2016
  • Published online February 9, 2016.
Copyright & Usage 
Copyright © 2016 Thomas et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Ajay X. Thomas1,2,3,
  2. Yasmin Cruz Del Angel1,
  3. Marco I. Gonzalez1,2,
  4. Andrew J. Carrel1,2,
  5. Jessica Carlsen1,
  6. Philip M. Lam1,
  7. Barbara L. Hempstead4,
  8. Shelley. J. Russek5 and
  9. Amy R. Brooks-Kayal1,2,6
  1. 1Department of Pediatrics, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045
  2. 2Neuroscience Program, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045
  3. 3Medical Scientist Training Program, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045
  4. 4Departments of Medicine, Hematology & Medical Oncology, Weil Cornell Medical College, New York, New York 10045
  5. 5Department of Pharmacology, Boston University School of Medicine, Boston, Massachusetts 02118
  6. 6Department of Neurology, Children’s Hospital Colorado, Aurora, Colorado 80045
  1. Correspondence should be addressed to Amy Brooks-Kayal, Department of Pediatrics, University of Colorado Anschutz Medical Campus, Aurora, CO 80045. E-mail: Amy.Brooks-Kayal{at}childrenscolorado.org.
View Full Text

Author contributions

  1. ↵2 Author Contributions: A.X.T., M.I.G., A.J.C., J.C., S.J.R., and A.R.B.-K. designed research; A.X.T., Y.C.D.A., A.J.C., J.C., and P.M.L. performed research; A.X.T., Y.C.D.A., and A.R.B.-K. analyzed data; A.X.T., M.I.G., B.L.H., and A.R.B.-K. wrote the paper; B.L.H. contributed unpublished reagents/analytic tools.

View Abstract

Disclosures

  • ↵1 The authors declare no competing financial interests.

  • ↵3 The studies were supported by National Institute of Neurological Disorders and Stroke funding, as follows: Grant 5R01-NS-051710 to S.J.R. and A.R.B.K., Grant 5R01-NS-030687 to B.L.H., and Grant F31-NS-078844 to A.X.T.; and by Epilepsy Foundation/American Epilepsy Society funding to A.X.T. Imaging experiments were performed in the University of Colorado Anschutz Medical Campus Advance Light Microscopy Core, which is supported in part by Rocky Mountain Neurological Disorders Core Grant Number P30NS048154 and by National Institutes of Health/National Center for Research Resources Colorado Clinical and Translational Science Institute Grant UL1-RR-025780.

Funding

  • NINDS

    5R01NS051710
  • NINDS

    5R01NS030687
  • NINDS

    F31NS078844
  • NINDS

    P30NS048154
  • NIH/NCRR

    UL1 RR025780
  • Epilepsy Foundation/ American Epilepsy Society

Other Version

  • You are viewing the most recent version of this article.
  • previous version (February 09, 2016).

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Rapid Increases in proBDNF after Pilocarpine-Induced Status Epilepticus in Mice Are Associated with Reduced proBDNF Cleavage Machinery
Ajay X. Thomas, Yasmin Cruz Del Angel, Marco I. Gonzalez, Andrew J. Carrel, Jessica Carlsen, Philip M. Lam, Barbara L. Hempstead, Shelley. J. Russek, Amy R. Brooks-Kayal
eNeuro 9 February 2016, 3 (1) ENEURO.0020-15.2016; DOI: 10.1523/ENEURO.0020-15.2016

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Rapid Increases in proBDNF after Pilocarpine-Induced Status Epilepticus in Mice Are Associated with Reduced proBDNF Cleavage Machinery
Ajay X. Thomas, Yasmin Cruz Del Angel, Marco I. Gonzalez, Andrew J. Carrel, Jessica Carlsen, Philip M. Lam, Barbara L. Hempstead, Shelley. J. Russek, Amy R. Brooks-Kayal
eNeuro 9 February 2016, 3 (1) ENEURO.0020-15.2016; DOI: 10.1523/ENEURO.0020-15.2016
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Keywords

  • BDNF
  • cleavage
  • epilepsy
  • PAI-1
  • ProBDNF
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