Article Figures & Data
Figures
- Figure 1.
Effects of aging on PER2::LUC rhythms in SCN explants from mice maintained under LD cycles. A, Representative double-plotted actograms of wheel-running activity in young and aged PER2::LUC mice maintained under LD cycles. Lighting conditions are indicated at the top of the figure; open bars indicate light phases, and closed bars indicate dark phases. Asterisks indicate the timing of the killing of the animals. B, Typical examples of the PER2::LUC rhythm of SCN explants from mice maintained under LD cycles, as measured by PMT. C, Phase map of peak PER2::LUC rhythms in the SCN. Each point represents the average peak of PER2::LUC rhythms in each cycle, plotted relative to the LD cycle prior to the killing of the animals. D, Amplitude of PER2::LUC rhythms in the SCN. The amplitude of each oscillation was determined as the sum of the absolute values of the lowest and highest points (counts). Data are shown as the mean ± SD. n = 5 per group.
- Figure 2.
Effects of aging on PER2::LUC rhythms in SCN explants from mice maintained in DD. A, Representative double-plotted actograms of wheel-running activity in young and aged PER2::LUC mice maintained in DD. Asterisks indicate the timing of the killing of the animals. B, Typical examples of PER2::LUC rhythms of SCN explants from mice maintained in DD for 10 d, as measured by PMT. C, Phase map of peak PER2::LUC rhythms in the SCN. Each point represents the average peak of PER2::LUC rhythms in each cycle, plotted relative to the circadian time prior to the killing of the animals. D, Amplitude of PER2::LUC rhythms in the SCN. The amplitude of each oscillation was determined as the sum of the absolute value of the lowest and highest points (counts). Data are shown as the mean ± SD. n = 5 per group. *p < 0.05, **p < 0.01 for young vs. aged animals (Bonferroni post hoc comparison).
- Figure 3.
Effects of aging on PER2::LUC rhythms in SCN explants from mice maintained in DD. A, Representative images of PER2::LUC in SCN explants collected from young and aged mice maintained in DD for 10 d, recorded by an EM-CCD camera at 30, 42, 108, and 120 h after the start of recording. B, Representative graphical (top) and raster (below) plots of PER2::LUC rhythms of individual cells in SCN explants of young and aged SCNs. C, D, Phase spreads (C) and normalized amplitudes (D) of PER2::LUC rhythms averaged over young (red) and aged (green) SCNs are shown over five cycles. E, Ratios of free-running circadian period change from the first to the fifth cycle of PER2::LUC rhythms in 50 individual SCN cells are shown. Data are shown as the mean ± SD. n = 6 per group. *p < 0.05 (t test). Scale bar, 100 μm.
Tables
- Table 1.
Statistical table: results of two-way repeated-measures ANOVA for datasets of phase and amplitude in PMT recordings (Figs. 1C,D, 2C,D), and datasets of phase spread and amplitude in EM-CCD camera recordings (Fig. 3C,D)
Interaction effect [age × cycle (time)] F value P-value Figure a Phase in LD F(4,32) = 0.141 0.966 1C b Amplitude in LD F(4,32) = 2.045 0.112 1D c Phase in DD F(4,32) = 11.328 <0.001 2C d Amplitude in DD F(4,32) = 12.377 <0.001 2D e Phase spread in camera F(71,710) = 3.968 <0.001 3C f Amplitude in camera F(71,710) = 0.997 0.488 3D Data structure Type of test p value Figure g Normally distributed Bonferroni 0.016 2C h Normally distributed Bonferroni 0.033 2C i Normally distributed Bonferroni 0.040 2C j Normally distributed Bonferroni 0.012 2C k Normally distributed Bonferroni 0.002 2C l Normally distributed Bonferroni 0.003 2D m Normally distributed Bonferroni 0.004 2D n Normally distributed Bonferroni 0.003 2D o Normally distributed Bonferroni 0.004 2D p Normally distributed t test 0.026 3E
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