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Research ArticleNegative Results, Disorders of the Nervous System

Hypoxia Inducible Factor-1α in Astrocytes and/or Myeloid Cells Is Not Required for the Development of Autoimmune Demyelinating Disease

Natacha Le Moan, Kim M. Baeten, Victoria A. Rafalski, Jae Kyu Ryu, Pamela E. Rios Coronado, Catherine Bedard, Catriona Syme, Dimitrios Davalos and Katerina Akassoglou
eNeuro 30 March 2015, 2 (2) ENEURO.0050-14.2015; DOI: https://doi.org/10.1523/ENEURO.0050-14.2015
Natacha Le Moan
1Gladstone Institute of Neurological Disease, University of California, San Francisco, San Francisco, CA 94158, USA
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Kim M. Baeten
1Gladstone Institute of Neurological Disease, University of California, San Francisco, San Francisco, CA 94158, USA
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  • ORCID record for Kim M. Baeten
Victoria A. Rafalski
1Gladstone Institute of Neurological Disease, University of California, San Francisco, San Francisco, CA 94158, USA
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Jae Kyu Ryu
1Gladstone Institute of Neurological Disease, University of California, San Francisco, San Francisco, CA 94158, USA
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Pamela E. Rios Coronado
1Gladstone Institute of Neurological Disease, University of California, San Francisco, San Francisco, CA 94158, USA
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Catherine Bedard
1Gladstone Institute of Neurological Disease, University of California, San Francisco, San Francisco, CA 94158, USA
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Catriona Syme
1Gladstone Institute of Neurological Disease, University of California, San Francisco, San Francisco, CA 94158, USA
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Dimitrios Davalos
1Gladstone Institute of Neurological Disease, University of California, San Francisco, San Francisco, CA 94158, USA
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Katerina Akassoglou
1Gladstone Institute of Neurological Disease, University of California, San Francisco, San Francisco, CA 94158, USA
2Department of Neurology, University of California, San Francisco, San Francisco, CA 94143, USA
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Article Information

DOI 
https://doi.org/10.1523/ENEURO.0050-14.2015
PubMed 
26213713
Published By 
Society for Neuroscience
History 
  • Received October 27, 2014
  • Revision received March 20, 2015
  • Accepted March 23, 2015
  • Published online March 30, 2015.
Copyright & Usage 
Copyright © 2015 Le Moan et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Natacha Le Moan1,
  2. Kim M. Baeten1,
  3. Victoria A. Rafalski1,
  4. Jae Kyu Ryu1,
  5. Pamela E. Rios Coronado1,
  6. Catherine Bedard1,
  7. Catriona Syme1,
  8. Dimitrios Davalos1 and
  9. Katerina Akassoglou1,2
  1. 1Gladstone Institute of Neurological Disease, University of California, San Francisco, San Francisco, CA 94158, USA
  2. 2Department of Neurology, University of California, San Francisco, San Francisco, CA 94143, USA
  1. Correspondence should be addressed to Katerina Akassoglou, Gladstone Institute of Neurological Disease and Department of Neurology, University of California, San Francisco, 1650 Owens Street, San Francisco, CA 94158. E-mail: kakassoglou{at}gladstone.ucsf.edu.
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Author contributions

  1. ↵2 N.L.M. and K.A. designed research; N.L.M. performed the EAE and hypoxia experiments; N.L.M., K.M.B., J.K.R, V.A.R, and P.E.R.C. performed research and analyzed data; C.S. analyzed data; C.B. maintained the mouse colony; D.D. performed image analysis; N.L.M. and K.A. wrote paper with contribution from all authors.

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Disclosures

  • ↵1 The authors declare no competing financial interests.

  • ↵3 This work was supported by a National Multiple Sclerosis Society postdoctoral fellowship to N.L.M. and NIH/NINDS Grants R01NS051470 and R01NS052189 to K.A. V.A.R is an American Heart Association (AHA) postdoctoral fellow and D.D. holds Scientist Development Grant 13SDG17210051 from AHA. The J. David Gladstone Institutes received support from a National Center for Research Resources (Grant RR18928).

Funding

  • National Institute of Neurologic Diseases and Stroke (NINDS)

    NS051470
  • National Institute of Neurologic Diseases and Stroke (NINDS)

    NS052189
  • National Multiple Sclerosis Society

    100000890
  • American Heart Association

    13SDG17210051

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Hypoxia Inducible Factor-1α in Astrocytes and/or Myeloid Cells Is Not Required for the Development of Autoimmune Demyelinating Disease
Natacha Le Moan, Kim M. Baeten, Victoria A. Rafalski, Jae Kyu Ryu, Pamela E. Rios Coronado, Catherine Bedard, Catriona Syme, Dimitrios Davalos, Katerina Akassoglou
eNeuro 30 March 2015, 2 (2) ENEURO.0050-14.2015; DOI: 10.1523/ENEURO.0050-14.2015

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Hypoxia Inducible Factor-1α in Astrocytes and/or Myeloid Cells Is Not Required for the Development of Autoimmune Demyelinating Disease
Natacha Le Moan, Kim M. Baeten, Victoria A. Rafalski, Jae Kyu Ryu, Pamela E. Rios Coronado, Catherine Bedard, Catriona Syme, Dimitrios Davalos, Katerina Akassoglou
eNeuro 30 March 2015, 2 (2) ENEURO.0050-14.2015; DOI: 10.1523/ENEURO.0050-14.2015
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Keywords

  • astrocytes
  • Cre/loxP
  • EAE
  • HIF-1alpha
  • macrophages
  • neuroinflammation

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