Figure 6 Modulation of nicotine-evoked Ca2+-transient with CNG disruption. A−D, Bioluminescent Ca2+-activity evoked by nicotine in CNGs knocked-down using the following: cngc-RNAi(1) (n = 11, A), cngc-RNAi(2) (n = 9, B), cngl-RNAi(1) (continued in page 16). (n = 6, C), and cngl-RNAi(2) (n = 7, D). E, G, I, Bioluminescent Ca2+-activity in CNGC knockdown with upregulated cAMP production in Gαs* (n = 5) transgenic flies (E), flies incubated for 10 min with forskolin (13 μM, n = 7, F), and flies incubated for 10 min with 8Br-cAMP (200 μM, n = 7, I). F, H, J, Bioluminescent Ca2+-activity in CNGL knockdown with upregulated cAMP production in Gαs* (n = 11) transgenic flies (F), flies incubated for 10 min with forskolin (13 μM, n = 8, H), and flies incubated for 10 min with 8-Br-cAMP (200 μM, n = 8, J). K, M, Bioluminescent Ca2+-activity in CNGC knockdown with upregulated PKA activity in UAS-mC* (n = 8, K) and downregulated PKA activity in UAS-R* (n = 9, M). L, N, Bioluminescent Ca2+-activity in CNGL knockdown with upregulated PKA activity in UAS-mC* (n = 9, L) and downregulated PKA activity in UAS-R* (n = 9, N). O, Bioluminescent image (accumulation time: 120 s) of the nicotine-evoked response in a typical fly of each genotype.