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Research ArticleMethods/New Tools, Novel Tools and Methods

3D Imaging of Axons in Transparent Spinal Cords from Rodents and Nonhuman Primates

Cynthia Soderblom, Do-Hun Lee, Abdul Dawood, Melissa Carballosa, Andrea Jimena Santamaria, Francisco D. Benavides, Stanislava Jergova, Robert M. Grumbles, Christine K. Thomas, Kevin K. Park, James David Guest, Vance P. Lemmon, Jae K. Lee and Pantelis Tsoulfas
eNeuro 26 March 2015, 2 (2) ENEURO.0001-15.2015; DOI: https://doi.org/10.1523/ENEURO.0001-15.2015
Cynthia Soderblom
Miami Project to Cure Paralysis, Department of Neurological Surgery, University of Miami School of Medicine, Miami, Florida 33136
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Do-Hun Lee
Miami Project to Cure Paralysis, Department of Neurological Surgery, University of Miami School of Medicine, Miami, Florida 33136
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Abdul Dawood
Miami Project to Cure Paralysis, Department of Neurological Surgery, University of Miami School of Medicine, Miami, Florida 33136
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Melissa Carballosa
Miami Project to Cure Paralysis, Department of Neurological Surgery, University of Miami School of Medicine, Miami, Florida 33136
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Andrea Jimena Santamaria
Miami Project to Cure Paralysis, Department of Neurological Surgery, University of Miami School of Medicine, Miami, Florida 33136
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  • ORCID record for Andrea Jimena Santamaria
Francisco D. Benavides
Miami Project to Cure Paralysis, Department of Neurological Surgery, University of Miami School of Medicine, Miami, Florida 33136
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Stanislava Jergova
Miami Project to Cure Paralysis, Department of Neurological Surgery, University of Miami School of Medicine, Miami, Florida 33136
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Robert M. Grumbles
Miami Project to Cure Paralysis, Department of Neurological Surgery, University of Miami School of Medicine, Miami, Florida 33136
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Christine K. Thomas
Miami Project to Cure Paralysis, Department of Neurological Surgery, University of Miami School of Medicine, Miami, Florida 33136
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Kevin K. Park
Miami Project to Cure Paralysis, Department of Neurological Surgery, University of Miami School of Medicine, Miami, Florida 33136
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James David Guest
Miami Project to Cure Paralysis, Department of Neurological Surgery, University of Miami School of Medicine, Miami, Florida 33136
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Vance P. Lemmon
Miami Project to Cure Paralysis, Department of Neurological Surgery, University of Miami School of Medicine, Miami, Florida 33136
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Jae K. Lee
Miami Project to Cure Paralysis, Department of Neurological Surgery, University of Miami School of Medicine, Miami, Florida 33136
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Pantelis Tsoulfas
Miami Project to Cure Paralysis, Department of Neurological Surgery, University of Miami School of Medicine, Miami, Florida 33136
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Article Information

DOI 
https://doi.org/10.1523/ENEURO.0001-15.2015
Published By 
Society for Neuroscience
History 
  • Received January 5, 2015
  • Revision received March 16, 2015
  • Accepted March 17, 2015
  • Published online March 26, 2015.
Copyright & Usage 
Copyright © 2015 Soderblom et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Cynthia Soderblom*,
  2. Do-Hun Lee*,
  3. Abdul Dawood,
  4. Melissa Carballosa,
  5. Andrea Jimena Santamaria,
  6. Francisco D. Benavides,
  7. Stanislava Jergova,
  8. Robert M. Grumbles,
  9. Christine K. Thomas,
  10. Kevin K. Park,
  11. James David Guest,
  12. Vance P. Lemmon,
  13. Jae K. Lee and
  14. Pantelis Tsoulfas
  1. Miami Project to Cure Paralysis, Department of Neurological Surgery, University of Miami School of Medicine, Miami, Florida 33136
  1. Correspondence should be addressed to either of the following: Pantelis Tsoulfas, University of Miami School of Medicine, Miami Project to Cure Paralysis, Department of Neurological Surgery, 1095 NW 14th Terrace, LPLC 4-21, Miami, FL 33136, E-mail: PTsoulfa{at}med.miami.edu; or Jae K. Lee, University of Miami School of Medicine, Miami Project to Cure Paralysis, Department of Neurological Surgery, 1095 NW 14th Terrace, LPLC 4-19, Miami, FL 33136, E-mail: JLee22{at}med.miami.edu.
View Full Text

Author contributions

  1. ↵2 Author Contributions: C.S., D.H.L., M.C., A.J.S., F.D.B., S.J., R.G., C.T., K.K.P., J.D.G., V.L., J.K.L., and P.T. designed research; C.S., D.H.L., A.D., M.C., A.J.S., F.D.B., S.J., R.G., J.D.G., J.K.L., and P.T. performed research; C.S., D.H.L., A.D., A.J.S., F.D.B., S.J., R.G., J.D.G., and P.T. analyzed data; C.S., D.H.L., A.D., M.C., A.J.S., S.J., R.G., C.T., K.K.P., J.D.G., V.L., J.K.L., and P.T. wrote the paper.

  2. ↵* C.S. and D.H.L. contributed equally to this work.

View Abstract

Disclosures

  • ↵1 The authors declare no competing financial interests.

  • ↵3 This study was funded by NINDS Grants R01NS081040 and R21NS082835, NEI Grant R01EY022961, NICHD Grant RO1HD057632, Craig Neilsen Foundation, Department of Defense, International Spinal Research Trust, State of Florida Specific Appropriation 538, The Miami Project to Cure Paralysis, and the Buoniconti Fund.

Funding

  • NINDS

    R01NS081040
  • NINDS

    R21NS082835
  • NEI

  • Craig Neilsen Foundation

  • Department of Defense

  • International Spinal Research Trust

  • State of Florida Specific Appropriation 538

  • The Miami Project to Cure Paralysis

  • The Buoniconti Fund

  • HHS | NIH | National Institute of Child Health and Human Development (NICHD)

    100000071; R01HD057632

Other Version

  • You are viewing the most recent version of this article.
  • previous version (March 26, 2015).

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3D Imaging of Axons in Transparent Spinal Cords from Rodents and Nonhuman Primates
Cynthia Soderblom, Do-Hun Lee, Abdul Dawood, Melissa Carballosa, Andrea Jimena Santamaria, Francisco D. Benavides, Stanislava Jergova, Robert M. Grumbles, Christine K. Thomas, Kevin K. Park, James David Guest, Vance P. Lemmon, Jae K. Lee, Pantelis Tsoulfas
eNeuro 26 March 2015, 2 (2) ENEURO.0001-15.2015; DOI: 10.1523/ENEURO.0001-15.2015

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3D Imaging of Axons in Transparent Spinal Cords from Rodents and Nonhuman Primates
Cynthia Soderblom, Do-Hun Lee, Abdul Dawood, Melissa Carballosa, Andrea Jimena Santamaria, Francisco D. Benavides, Stanislava Jergova, Robert M. Grumbles, Christine K. Thomas, Kevin K. Park, James David Guest, Vance P. Lemmon, Jae K. Lee, Pantelis Tsoulfas
eNeuro 26 March 2015, 2 (2) ENEURO.0001-15.2015; DOI: 10.1523/ENEURO.0001-15.2015
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Keywords

  • 3DISCO
  • axon regeneration
  • spinal cord injury
  • tissue clearing

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