Article Figures & Data
Figures
- Fig. 1
The effect of α9a10 nAChR blockers Vc1.1 and RgIA on CaV currents in rat DRG neurons. A, Superimposed traces of Ba2+ currents from one cell in the absence (grey) and presence (black) of 30 µM baclofen, 1µM Vc1.1, and 1 µM RgIA. Voltage protocol is shown at the bottom. B, The blocking time course of prepulse (filled circles) and postpulse (empty circles) current by baclofen, Vc1.1, and RgIA. C, The plot shows mean percent CaV current inhibition by baclofen (n = 21), Vc1.1 (n = 21), and RgIA (n = 12), along with the individual data points to illustrate the large variability in responses. * indicates significant inhibition (p < 0.05).
- Fig. 2
Rapid recovery from Vc1.1-induced inhibition. A, Example traces from a neuron with a 40% inhibition of CaV current induced by 1 µM Vc1.1 (blue trace). Note the almost full recovery from inhibition in the washout trace (Wash). B, The time course of inhibition by Vc1.1. The asterisks indicate the traces used in A. C, The average time constant (τ) for recovery from block by 1 µM Vc1.1 from the four neurons with inhibition >10%. Recovery τ was determined by fitting the Vc1.1 washout time course using a single exponential equation.
- Fig. 3
Inhibition by α-conopeptides Vc1.1 or RgIA does not correlate with inhibition by the GABAB agonist baclofen. A, The percent inhibition of prepulse CaV current by 30 µM baclofen, 1 µM Vc1.1, or 1 µM RgIA is plotted versus cell diameter (calculated as described in Materials and Methods). B, The percent inhibition of prepulse CaV current by α-conopeptides Vc1.1 or RgIA is plotted versus percent inhibition by baclofen, and no correlation was observed.
- Fig. 4
Vc1.1 and RgIA potently block α9α10 nAChRs expressed in X. laevis oocytes. ACh-induced currents were measured from voltage-clamped oocytes as described in Materials and Methods. A, B, Representative traces of ACh-induced currents in the presence and absence of Vc1.1 or RgIA, respectively. C, The mean (±SD) inhibition of ACh-induced peak current amplitude by α-conopeptides Vc1.1 and RgIA. The numbers in bars reflects numbers of cells tested. * indicates significant inhibition (p < 0.05).
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