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Research ArticleResearch Article: Methods/New Tools, Novel Tools and Methods

Reliable Inference of the Encoding of Task States by Individual Neurons Using Calcium Imaging

Huixin Huang, Garima Shah, Hita Adwanikar and Shreesh P. Mysore
eNeuro 20 January 2026, 13 (1) ENEURO.0378-25.2025; https://doi.org/10.1523/ENEURO.0378-25.2025
Huixin Huang
1Department of Psychological and Brain Sciences, Johns Hopkins University, Baltimore, Maryland 21218
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Garima Shah
1Department of Psychological and Brain Sciences, Johns Hopkins University, Baltimore, Maryland 21218
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Hita Adwanikar
1Department of Psychological and Brain Sciences, Johns Hopkins University, Baltimore, Maryland 21218
2The Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine, Baltimore, Maryland 21218
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Shreesh P. Mysore
1Department of Psychological and Brain Sciences, Johns Hopkins University, Baltimore, Maryland 21218
2The Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine, Baltimore, Maryland 21218
3Kavli Neuroscience Discovery Institute, Johns Hopkins University, Baltimore, Maryland 21218
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Article Information

DOI 
https://doi.org/10.1523/ENEURO.0378-25.2025
PubMed 
41558962
Published By 
Society for Neuroscience
History 
  • Received October 12, 2025
  • Revision received December 14, 2025
  • Accepted December 17, 2025
  • Published online January 20, 2026.
Copyright & Usage 
Copyright © 2026 Huang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Huixin Huang1,*,
  2. Garima Shah1,*,
  3. Hita Adwanikar1,2,** and
  4. Shreesh P. Mysore1,2,3,**
  1. 1Department of Psychological and Brain Sciences, Johns Hopkins University, Baltimore, Maryland 21218
  2. 2The Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine, Baltimore, Maryland 21218
  3. 3Kavli Neuroscience Discovery Institute, Johns Hopkins University, Baltimore, Maryland 21218
  1. Correspondence should be addressed to Shreesh P. Mysore at mysore{at}jhu.edu.
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Author contributions

  1. Author contributions: H.A. and S.P.M. designed research; H.H. and G.S. performed research; H.H., H.A., and S.P.M. contributed unpublished reagents/analytic tools; H.H. analyzed data; H.H., H.A., and S.P.M. wrote the paper.

Disclosures

  • The authors declare no competing financial interests.

  • We thank the members of the Mysore and Adwanikar groups for feedback and discussions. This work was supported by startup funds from the Johns Hopkins University (S.P.M. and H.A.).

  • ↵*H.H. and G.S. are co-first authors with equal contribution.

  • ↵**H.A. and S.P.M. are co-corresponding authors with equal contribution.

  • G.S.’s present address: Kearney, Canada.

Funding

  • Johns Hopkins University (JHU)

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Reliable Inference of the Encoding of Task States by Individual Neurons Using Calcium Imaging
Huixin Huang, Garima Shah, Hita Adwanikar, Shreesh P. Mysore
eNeuro 20 January 2026, 13 (1) ENEURO.0378-25.2025; DOI: 10.1523/ENEURO.0378-25.2025

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Reliable Inference of the Encoding of Task States by Individual Neurons Using Calcium Imaging
Huixin Huang, Garima Shah, Hita Adwanikar, Shreesh P. Mysore
eNeuro 20 January 2026, 13 (1) ENEURO.0378-25.2025; DOI: 10.1523/ENEURO.0378-25.2025
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Keywords

  • calcium imaging
  • encoding
  • medial prefrontal cortex
  • neuron
  • selectivity
  • task states

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