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Research ArticleOpen Source Tools and Methods, Novel Tools and Methods

Track-A-Worm 2.0: A Software Suite for Quantifying Properties of C. elegans Locomotion, Bending, Sleep, and Action Potentials

Kiranmayi Vedantham, Longgang Niu, Ryan Ma, Liam Connelly, Anusha Nagella, Sijie Jason Wang and Zhao-Wen Wang
eNeuro 27 August 2025, 12 (8) ENEURO.0224-25.2025; https://doi.org/10.1523/ENEURO.0224-25.2025
Kiranmayi Vedantham
1Department of Neuroscience, University of Connecticut School of Medicine, Farmington, Connecticut 06030
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Longgang Niu
1Department of Neuroscience, University of Connecticut School of Medicine, Farmington, Connecticut 06030
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Ryan Ma
2Health Research Program, University of Connecticut, Storrs, Connecticut 06030
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Liam Connelly
2Health Research Program, University of Connecticut, Storrs, Connecticut 06030
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Anusha Nagella
2Health Research Program, University of Connecticut, Storrs, Connecticut 06030
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Sijie Jason Wang
3MD Program, University of Connecticut School of Medicine, Farmington, Connecticut 06030
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Zhao-Wen Wang
1Department of Neuroscience, University of Connecticut School of Medicine, Farmington, Connecticut 06030
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Article Information

DOI 
https://doi.org/10.1523/ENEURO.0224-25.2025
PubMed 
40866299
Published By 
Society for Neuroscience
History 
  • Received June 11, 2025
  • Revision received July 11, 2025
  • Accepted July 25, 2025
  • Published online August 27, 2025.
Copyright & Usage 
Copyright © 2025 Vedantham et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Kiranmayi Vedantham1,
  2. Longgang Niu1,
  3. Ryan Ma2,
  4. Liam Connelly2,
  5. Anusha Nagella2,
  6. Sijie Jason Wang3 and
  7. Zhao-Wen Wang1
  1. 1Department of Neuroscience, University of Connecticut School of Medicine, Farmington, Connecticut 06030
  2. 2Health Research Program, University of Connecticut, Storrs, Connecticut 06030
  3. 3MD Program, University of Connecticut School of Medicine, Farmington, Connecticut 06030
  1. Correspondence should be addressed to Zhao-Wen Wang at zwwang{at}uchc.edu.
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Author contributions

  1. Author contributions: K.V., S.J.W., and Z.-W.W. designed the research; K.V., L.N., L.C., A.N., and S.J.W. wrote the software codes; K.V. and R.M. created the videos; R.M. and L.N. conducted the locomotion recordings and analyzed the data; K.V., S.J.W., and Z-W.W. wrote the manuscript.

Disclosures

  • The authors declare no competing financial interests.

  • This work was supported by the National Institutes of Health R01MH085927 (to Z-W.W.). We thank the Caenorhabditis Genetics Center for mutant strains.

Funding

  • HHS | NIH | National Institute of Mental Health (NIMH)

    R01MH085927

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eneuro: 12 (8)
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August 2025
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Track-A-Worm 2.0: A Software Suite for Quantifying Properties of C. elegans Locomotion, Bending, Sleep, and Action Potentials
Kiranmayi Vedantham, Longgang Niu, Ryan Ma, Liam Connelly, Anusha Nagella, Sijie Jason Wang, Zhao-Wen Wang
eNeuro 27 August 2025, 12 (8) ENEURO.0224-25.2025; DOI: 10.1523/ENEURO.0224-25.2025

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Track-A-Worm 2.0: A Software Suite for Quantifying Properties of C. elegans Locomotion, Bending, Sleep, and Action Potentials
Kiranmayi Vedantham, Longgang Niu, Ryan Ma, Liam Connelly, Anusha Nagella, Sijie Jason Wang, Zhao-Wen Wang
eNeuro 27 August 2025, 12 (8) ENEURO.0224-25.2025; DOI: 10.1523/ENEURO.0224-25.2025
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  • Article
    • Abstract
    • Significance Statement
    • Introduction
    • Materials and Methods
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Keywords

  • action potential
  • C. elegans
  • locomotion
  • MATLAB
  • open-source
  • sleep

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