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Research ArticleResearch Article: New Research, Novel Tools and Methods

Single-Cell Approaches Define the Murine Leptomeninges: Cortical Brain Interface as a Distinct Cellular Neighborhood Composed of Neural and Non-neural Cell Types

Sarah N. Ebert, Christine Eisner, Konstantina Karamboulas, Louis-Philippe Bernier, David R. Kaplan, Brian A. MacVicar and Freda D. Miller
eNeuro 8 August 2025, 12 (8) ENEURO.0046-25.2025; https://doi.org/10.1523/ENEURO.0046-25.2025
Sarah N. Ebert
1Neuroscience Graduate Program, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
2Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada
3Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
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Christine Eisner
2Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada
4Program in Neurosciences and Mental Health, Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada
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Konstantina Karamboulas
4Program in Neurosciences and Mental Health, Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada
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Louis-Philippe Bernier
3Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
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David R. Kaplan
4Program in Neurosciences and Mental Health, Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada
5Departments of Medical Genetics, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
7Department of Molecular Genetics, University of Toronto, Toronto, Ontario M5S 1A8, Canada
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Brian A. MacVicar
1Neuroscience Graduate Program, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
3Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
6Psychiatry, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
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Freda D. Miller
1Neuroscience Graduate Program, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
2Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada
3Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
4Program in Neurosciences and Mental Health, Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada
5Departments of Medical Genetics, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
7Department of Molecular Genetics, University of Toronto, Toronto, Ontario M5S 1A8, Canada
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eneuro: 12 (8)
eNeuro
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August 2025
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Single-Cell Approaches Define the Murine Leptomeninges: Cortical Brain Interface as a Distinct Cellular Neighborhood Composed of Neural and Non-neural Cell Types
Sarah N. Ebert, Christine Eisner, Konstantina Karamboulas, Louis-Philippe Bernier, David R. Kaplan, Brian A. MacVicar, Freda D. Miller
eNeuro 8 August 2025, 12 (8) ENEURO.0046-25.2025; DOI: 10.1523/ENEURO.0046-25.2025

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Single-Cell Approaches Define the Murine Leptomeninges: Cortical Brain Interface as a Distinct Cellular Neighborhood Composed of Neural and Non-neural Cell Types
Sarah N. Ebert, Christine Eisner, Konstantina Karamboulas, Louis-Philippe Bernier, David R. Kaplan, Brian A. MacVicar, Freda D. Miller
eNeuro 8 August 2025, 12 (8) ENEURO.0046-25.2025; DOI: 10.1523/ENEURO.0046-25.2025
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Keywords

  • astrocytes
  • layer 1 cortex
  • meninges
  • spatial transcriptomics

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