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Research ArticleResearch Article: New Research, Cognition and Behavior

Calcium Dynamics in Hypothalamic Paraventricular Oxytocin Neurons and Astrocytes Associated with Social and Stress Stimuli

Katy Celina Sandoval, Joshua Rychlik and Katrina Y. Choe
eNeuro 22 April 2025, 12 (5) ENEURO.0196-24.2025; https://doi.org/10.1523/ENEURO.0196-24.2025
Katy Celina Sandoval
Department of Psychology, Neuroscience and Behaviour, McMaster University, Hamilton, Ontario L8S 4K1, Canada
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Joshua Rychlik
Department of Psychology, Neuroscience and Behaviour, McMaster University, Hamilton, Ontario L8S 4K1, Canada
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Katrina Y. Choe
Department of Psychology, Neuroscience and Behaviour, McMaster University, Hamilton, Ontario L8S 4K1, Canada
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Article Information

DOI 
https://doi.org/10.1523/ENEURO.0196-24.2025
PubMed 
40262904
Published By 
Society for Neuroscience
History 
  • Received May 6, 2024
  • Revision received April 13, 2025
  • Accepted April 17, 2025
  • Published online April 22, 2025.
Copyright & Usage 
Copyright © 2025 Sandoval et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Katy Celina Sandoval*,
  2. Joshua Rychlik* and
  3. Katrina Y. Choe
  1. Department of Psychology, Neuroscience and Behaviour, McMaster University, Hamilton, Ontario L8S 4K1, Canada
  1. Correspondence should be addressed to Katrina Y. Choe at choek{at}mcmaster.ca.
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Author contributions

  1. Author contributions: K.C.S., J.R., and K.Y.C. designed research; K.C.S., J.R., and K.Y.C. performed research; K.C.S., J.R., and K.Y.C. analyzed data; K.C.S., J.R., and K.Y.C. wrote the paper.

Disclosures

  • The authors declare no competing financial interests.

  • We thank L. Molina for assistance with troubleshooting associated with the FPA package, T. Fuzesi and J. Bains for helpful tips on setting up fiber photometry and the looming shadow task, and C. Bourque, M. Prager-Khoutorsky, and members of the Choe lab for their valuable feedback on the manuscript. We also thank A. Patwardhan for confocal imaging support, K. Andrec for genotyping assistance, and M. Matthew for assistance with experiments and colony management. Additionally, we thank D. Thompson for experimental setup support and D. Graham for animal care assistance. Confocal microscopy imaging made use of instrumentation available at the Centre for Advanced Light Microscopy (CALM) at McMaster University. K.Y.C. was supported by the Canada Foundation for Innovation, Ontario Research Fund, Canada Research Chair, Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery Grant, Department of Psychology, Neuroscience & Behaviour, and McMaster Startup Fund. K.C.S was supported by the NSERC PGS-D and McMaster CALM Seed Grant.

  • ↵*K.C.S. and J.R. contributed equally to this work.

Funding

  • Canadian Government | Natural Sciences and Engineering Research Council of Canada (NSERC)

  • Canada Foundation for Innovation (CFI)

  • Canada Research Chairs (Chaires de recherche du Canada)

  • McMaster University startup fund

  • Ontario Research Foundation (ORF)

  • McMaster CALM seed grant

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eneuro: 12 (5)
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May 2025
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Calcium Dynamics in Hypothalamic Paraventricular Oxytocin Neurons and Astrocytes Associated with Social and Stress Stimuli
Katy Celina Sandoval, Joshua Rychlik, Katrina Y. Choe
eNeuro 22 April 2025, 12 (5) ENEURO.0196-24.2025; DOI: 10.1523/ENEURO.0196-24.2025

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Calcium Dynamics in Hypothalamic Paraventricular Oxytocin Neurons and Astrocytes Associated with Social and Stress Stimuli
Katy Celina Sandoval, Joshua Rychlik, Katrina Y. Choe
eNeuro 22 April 2025, 12 (5) ENEURO.0196-24.2025; DOI: 10.1523/ENEURO.0196-24.2025
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Keywords

  • astrocytes
  • fiber photometry
  • oxytocin
  • social behavior
  • stress

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