Article Figures & Data
Figures
- Figure 1.
Spontaneous excitatory postsynaptic currents (sEPSCs) are larger in inhibitory LSO PNs. A, Example traces from 3 vGlut2 positive (excitatory) LSO PNs. B, Example traces from 3 vGlut2 negative (inhibitory) LSO PNs. C, Cumulative probability of sEPSC amplitudes. D, Amplitude of sEPSCs. E, Frequency of sEPSCs. F, sEPSC 20 to 80% rise time. G, sEPSC halfwidths. H, sEPSC decay tau (time constant). Cells (animals) E: n = 14(8), I: n = 15(12). Mean ± SEM. *p < 0.05, ***p < 0.001.
- Figure 2.
Evoked EPSCs (eEPSCs) exhibit similar amplitude, kinetics, and short-term plasticity in LSO PN types. A, Example eEPSC traces at minimal stimulation from 3 vGlut2+ (left, excitatory) and 3 vGlut2− (right, inhibitory) LSO PNs. B, Minimal eEPSC amplitude (all left and at expanded scale right). C, Minimal eEPSC 20–80% rise time. D, Minimal eEPSC halfwidth. E, Minimal eEPSC decay tau. B–E, Cell (animals) E: n = 14(10), I: n = 19(14). F, Example eEPSC traces for a pair of stimulations at minimal stimulation intensity with 5 ms interpulse interval (IPI) from 3 vGlut2+ (left traces) and 3 vGlut2− (right traces) LSO PNs. Scale is the same for all traces. Paired-pulse ratio (right). Open diamonds in graph are cells with p < 0.05 in bootstrap test for individual LSO PN's mean paired-pulse ratios. E: n = 5(5), I: n = 11(10). G, Same as F, for 10 ms IPI. E: n = 6(5), I: n = 13(11). H, 20 ms IPI. E: n = 8(7), I: n = 9(9). Mean ± SEM.
- Figure 3.
LSO PN types receive similar numbers of excitatory inputs. A, Example eEPSC amplitudes plotted against stimulation intensities for an excitatory LSO PN. Overlaid response traces shown in inset. Responses saturated at 1 mA. The number of inputs for the neuron was estimated as the number of peaks in a GMM fit to the histograms binned at 36 pA (solid line overlaying histogram), « symbols indicate the local maxima of the fit. B, Same as in A, but for an inhibitory LSO PN. C, The estimated number of inputs. E: n = 8(8), I: n = 6(6). D, Maximal amplitudes, measured as the largest amplitude at which the GMM fit had a peak. One cell that had a single estimated input but <100 pA amplitude was discarded from maximal stimulation analyses. E: n = 7(7), I: n = 6(6). Mean ± SEM.
- Figure 4.
Spontaneous inhibitory postsynaptic currents (sIPSCs) have larger amplitudes and slower decay kinetics in inhibitory LSO PNs. A, Example traces from 3 vGlut2 positive (excitatory) LSO PNs. High Cl− internal used. B, Example traces from 3 vGlut2 negative (inhibitory) LSO PNs. C, Cumulative probability of sIPSC amplitudes. D, Amplitude of sIPSCs. E, Frequency of sIPSCs. F, sIPSC 20 to 80% rise time. G, sIPSC halfwidths. H, sIPSC decay tau (time constant). Cells (animals) E: n = 20(15), I: n = 15(11). Mean ± SEM. **p < 0.01, ***p < 0.001, ****p < 0.0001.
- Figure 5.
Evoked IPSCs (eIPSCs) exhibit slower decay kinetics in inhibitory LSO PNs but similar amplitudes and short-term plasticity between LSO PN types. A, Example eIPSC traces at minimal stimulation from 3 vGlut2+ (left, excitatory) and 3 vGlut2− (right, inhibitory) LSO PNs. B, Minimal eIPSC amplitude (all left and at expanded scale right). C, Minimal eIPSC 20–80% rise time. D, Minimal eIPSC halfwidth. E, Minimal eIPSC decay tau. B–E, Cell(animals) E: n = 20(15), I: n = 13(10). F, Example eIPSC traces for a pair of stimulations at minimal stimulation intensity with 5-ms interpulse interval (IPI) from 3 vGlut2+ (left traces) and 3 vGlut2− (right traces) LSO PNs. Scale is the same for all traces. Paired-pulse ratio (right). Open diamonds in graph are cells with p < 0.05 in bootstrap test for individual LSO PN's mean paired-pulse ratios. E: n = 8(8), I: n = 4(4). G, Same as F, for 10 ms IPI. E: n = 11(8), I: n = 5(5). H, 20 ms IPI. E: n = 13(10), I: n = 8(8). Mean ± SEM. **p < 0.01, ***p < 0.001.
- Figure 6.
LSO PN types receive similar numbers of inhibitory inputs. A, Example eIPSC amplitudes plotted against stimulation intensities for an excitatory LSO PN. Overlaid response traces shown in inset. Responses saturated at 10 mA. The number of inputs for the neuron was estimated as the number of peaks in a GMM fit to the histograms binned at 93 pA (solid line overlaying histogram), « symbols indicate the local maxima of the fit. B, Same as in A, but for an inhibitory LSO PN. Responses saturated at 1 mA. C, The estimated number of inputs. D, Maximal amplitudes, measured as the largest amplitude at which GMM fit had a peak. E: n = 7(6), I: n = 10(7). Mean ± SEM.
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