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Research ArticleResearch Article: Negative Results, Sensory and Motor Systems

Paired Stimulation of Different Digits for 30 min Does Not Produce Long-Term Plastic Changes in the Human Cutaneomuscular Reflex

Maria Germann, Eldesta Nabila and Stuart N. Baker
eNeuro 20 March 2025, 12 (3) ENEURO.0103-24.2024; https://doi.org/10.1523/ENEURO.0103-24.2024
Maria Germann
Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne NE2 4HH, United Kingdom
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Eldesta Nabila
Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne NE2 4HH, United Kingdom
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Stuart N. Baker
Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne NE2 4HH, United Kingdom
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    Figure 1.

    Experimental design. A, Schematic representation of stimulation electrodes placement. B, Stimulation protocol for the assessments carried out before, 0–18 min after, and 18–36 min after (after2). C, Stimulation protocols for synchronous (top left), asynchronous (top right), 5 ms delay (bottom left), and thumb-only (bottom right) condition. ISI, interstimulus interval; ITI, intertrial interval.

  • Figure 2.
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    Figure 2.

    Overlay of EMG traces averaged across all subjects. The traces represent each assessment done before (black), 0–18 min after (orange), and 18–36 min after (cyan) the paired stimulation. Responses to thumb stimulation are plotted in the left column, responses to index finger stimulation are plotted in the right column. Gray vertical dotted lines indicate the three components (E1, I1, E2) of the cutaneoumuscular reflex. Gray horizontal dashed line represents the mean baseline value of the assessment done before the stimulation. The calibration bar is a percentage of the baseline EMG level. A, Overlay of averaged EMG traces for the synchronous stimulation protocol. B, Overlay of averaged EMG traces for the asynchronous stimulation protocol. C, Overlay of averaged EMG traces for the 5 ms delay stimulation protocol. D, Overlay of averaged EMG traces for the thumb-only stimulation protocol.

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    Figure 3.

    Group results showing MPC for the different stimulation protocols. Mean MPC for E1 (left), I1 (middle), and E2 (right) components of the CMR before, 0–18 min after (after), and 18–32 min after (after2) paired stimulation. Circles represent group mean for thumb (blue) and index (red) fingers; error bars indicate standard deviations. Asterisks indicate significant differences. Colored bars show the number of subjects showing an increase (yellow) or decrease (dark blue) after the stimulation protocol. Asterisks indicate proportions significantly different from the 50% expected by chance. A, Mean MPC and the number of subjects for the synchronous stimulation protocol. B, Mean MPC and the number of subjects for the asynchronous stimulation protocol. C, Mean MPC and number of subjects for the 5 ms delay stimulation protocol. D, Mean MPC and number of subjects for the thumb-only stimulation protocol.

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    Figure 4.

    Group results for the reflex summation experiment. A, Overlay of EMG traces averaged across all subjects, for the predicted (black) and the actual response (gray). Gray vertical dotted lines indicate the three components (E1, I1, E2) of the cutaneoumuscular reflex. Gray horizontal dashed line represents the mean baseline value of the average actual response trace. Calibration bar is a percentage of the baseline EMG level. B, Mean MPC for E1 (left), I1 (middle), and E2 (right) components of the CMR for the predicted response and the actual response. Single subjects are shown in black; group mean is shown in purple. Error bars indicate standard deviations. Asterisks indicate significant differences. Colored bars show the number of subjects showing an increased (yellow) or decreased (dark blue) MPC for the actual response compared with the predicted response.

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eneuro: 12 (3)
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Vol. 12, Issue 3
March 2025
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Paired Stimulation of Different Digits for 30 min Does Not Produce Long-Term Plastic Changes in the Human Cutaneomuscular Reflex
Maria Germann, Eldesta Nabila, Stuart N. Baker
eNeuro 20 March 2025, 12 (3) ENEURO.0103-24.2024; DOI: 10.1523/ENEURO.0103-24.2024

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Paired Stimulation of Different Digits for 30 min Does Not Produce Long-Term Plastic Changes in the Human Cutaneomuscular Reflex
Maria Germann, Eldesta Nabila, Stuart N. Baker
eNeuro 20 March 2025, 12 (3) ENEURO.0103-24.2024; DOI: 10.1523/ENEURO.0103-24.2024
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Keywords

  • cutaneomuscular reflex
  • spike-timing–dependent plasticity

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