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Research ArticleResearch Article: Methods/New Tools, Novel Tools and Methods

A Simple, Low-Cost Implant for Reliable Diaphragm EMG Recordings in Awake, Behaving Rats

Taylor C. Holmes, Jesus D. Penaloza-Aponte, Alyssa R. Mickle, Rachel L. Nosacka, Erica A. Dale and Kristi A. Streeter
eNeuro 31 January 2025, 12 (2) ENEURO.0444-24.2025; https://doi.org/10.1523/ENEURO.0444-24.2025
Taylor C. Holmes
1Exercise and Rehabilitation Science Program, Department of Physical Therapy, Marquette University, Milwaukee, Wisconsin 53233
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Jesus D. Penaloza-Aponte
2Department of Neuroscience, University of Florida, Gainesville, Florida 32610
3Breathing Research and Therapeutics Center, University of Florida, Gainesville, Florida 32610
4McKnight Brain Institute, University of Florida, Gainesville, Florida
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  • ORCID record for Jesus D. Penaloza-Aponte
Alyssa R. Mickle
2Department of Neuroscience, University of Florida, Gainesville, Florida 32610
3Breathing Research and Therapeutics Center, University of Florida, Gainesville, Florida 32610
4McKnight Brain Institute, University of Florida, Gainesville, Florida
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Rachel L. Nosacka
5Departments of Pediatrics, University of Florida, Gainesville, Florida 32610
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Erica A. Dale
2Department of Neuroscience, University of Florida, Gainesville, Florida 32610
3Breathing Research and Therapeutics Center, University of Florida, Gainesville, Florida 32610
4McKnight Brain Institute, University of Florida, Gainesville, Florida
6Physiology and Aging, University of Florida, Gainesville, Florida 32610
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Kristi A. Streeter
1Exercise and Rehabilitation Science Program, Department of Physical Therapy, Marquette University, Milwaukee, Wisconsin 53233
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Article Information

DOI 
https://doi.org/10.1523/ENEURO.0444-24.2025
PubMed 
39890457
Published By 
Society for Neuroscience
History 
  • Received October 14, 2024
  • Revision received January 24, 2025
  • Accepted January 27, 2025
  • Published online January 31, 2025.
Copyright & Usage 
Copyright © 2025 Holmes et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Taylor C. Holmes1,*,
  2. Jesus D. Penaloza-Aponte2,3,4,*,
  3. Alyssa R. Mickle2,3,4,
  4. Rachel L. Nosacka5,
  5. Erica A. Dale2,3,4,6 and
  6. Kristi A. Streeter1
  1. 1Exercise and Rehabilitation Science Program, Department of Physical Therapy, Marquette University, Milwaukee, Wisconsin 53233
  2. 2Department of Neuroscience, University of Florida, Gainesville, Florida 32610
  3. 3Breathing Research and Therapeutics Center, University of Florida, Gainesville, Florida 32610
  4. 4McKnight Brain Institute, University of Florida, Gainesville, Florida
  5. 5Departments of Pediatrics, University of Florida, Gainesville, Florida 32610
  6. 6Physiology and Aging, University of Florida, Gainesville, Florida 32610
  1. Correspondence should be addressed to Kristi A. Streeter at kristi.streeter{at}marquette.edu.
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Author contributions

  1. Author contributions: T.C.H., J.D.P.-A., A.R.M., R.L.N., E.A.D., and K.A.S. designed research; T.C.H., J.D.P.-A., A.R.M., R.L.N., E.A.D., and K.A.S. performed research; T.C.H. and J.D.P.-A. analyzed data; T.C.H., J.D.P.-A., R.L.N., E.A.D., and K.A.S. wrote the paper.

Disclosures

  • The authors declare no competing financial interests.

  • This work was supported by funding from National Institute of Health Grant Numbers National Heart, Lung, and Blood Institute R01HL153102 (to E.A.D.), T32HL134621 (to BREATHE Center, Mitchell PI; A.R.M.), and SPARC OT2OD023854 (to Bolser/E.A.D.);

  • National Heart Lung and Blood Institute, R00HL143207 (to K.A.S.); National Center for Advancing Translational Sciences, UL1TR001436 (to T.C.H.) and 2TL1TR001437 (to T.C.H.); Craig H. Neilsen Foundation Pilot Grant 993667 (to K.A.S.); and the Craig H Neilsen Foundation (pilot grant to E.A.D.).

  • ↵*T.C.H. and J.D.P.-A. are first co-authors.

Funding

  • HHS | NIH | National Heart, Lung, and Blood Institute (NHLBI)

    R01HL153102; T32HL134621; R00HL143207
  • HHS | NIH | National Center for Advancing Translational Sciences (NCATS)

    UL1TR001436; 2TL1TR001437
  • Craig H Neilsen Foundation - Pilot Grant

    993667
  • Stimulating Peripheral Activity to Relieve Conditions Program (SPARC)

    OT20OD023854

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A Simple, Low-Cost Implant for Reliable Diaphragm EMG Recordings in Awake, Behaving Rats
Taylor C. Holmes, Jesus D. Penaloza-Aponte, Alyssa R. Mickle, Rachel L. Nosacka, Erica A. Dale, Kristi A. Streeter
eNeuro 31 January 2025, 12 (2) ENEURO.0444-24.2025; DOI: 10.1523/ENEURO.0444-24.2025

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A Simple, Low-Cost Implant for Reliable Diaphragm EMG Recordings in Awake, Behaving Rats
Taylor C. Holmes, Jesus D. Penaloza-Aponte, Alyssa R. Mickle, Rachel L. Nosacka, Erica A. Dale, Kristi A. Streeter
eNeuro 31 January 2025, 12 (2) ENEURO.0444-24.2025; DOI: 10.1523/ENEURO.0444-24.2025
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Keywords

  • breathing
  • chronic recordings
  • diaphragm
  • electromyography
  • respiratory muscle

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