Article Figures & Data
Figures
- Figure 1.
Materials needed and assembly of recording chamber. A, The materials needed to construct 3D-printed recording chamber. B, Top of recording chamber before and after removal of brim support. C, Bottom of recording chamber before and after removal of brim and supports. D, E, Top and bottom, respectively, of finished recording chamber with polyethylene tubing and cover glass.
- Figure 2.
Materials needed and assembly of 3D-printed recording stage. A, The materials needed to construct 3D-printed recording stage. B, Recording stage components prepared for assembly with supports removed. C, Recording stage assembled with M6 hardware. D, Recording stage with recording chamber insert in place.
- Figure 3.
Example of possible recording chamber and stage features. A, Wide brim for electrode access. B, Slot for inflow tubing. C, Notch for outflow access. D, Channels for reference wires. E, Oblong well for high flow rate perfusion. F, Screw hole for magnetic outflow attachment. G, Notch for inflow tubing. H, Holes for attachment to legs. I, Holes allowing for easy removal of recording chamber insert.
- Figure 4.
Materials needed and assembly of 3D-printed tissue holding chamber. A, Required materials for 3D-printed tissue holding chamber. Cheesecloth can be substituted for a finer fabric for smaller tissue samples. B, Inner shell with doubled-over cheesecloth. C, Outer shell slid over inner shell with cheesecloth stretched between the layers. D, Assembled holding chamber including dividers.
- Figure 5.
3D-printed holding chamber and stage in use for LFP recordings in a mouse neocortical tissue. A, Holding chamber in use with carbogenated aCSF maintaining health of mouse brain slices. B, Recording stage and chamber in use with perfusion system, reference electrode, LFP microelectrode, digital microscope, and picospritzer.
- Figure 6.
Representative data using 3D-printed holding chamber and stage with the mouse cortical tissue and zebrafish optic tectum. Ai, Image showing a mouse neocortical and hippocampal tissue slice in the recording chamber secured by a platinum harp. The electrode placement in the neocortical tissue is shown. Aii, Electrophysiological trace displaying a spreading depolarization in the mouse neocortical tissue induced with a high-potassium bath (12 mM) aided by a high-potassium microinjection (100 mM, ∼20 pl). Bi, Image showing a zebrafish whole brain in a recording chamber secured by a harp. Electrode and picospritzer placement in the optic tectum is shown. Bii, Electrophysiological trace displaying a spreading depolarization in the zebrafish optic tectum induced by high-potassium microinjection (100 mM, ∼20 pl).
Tables
- Table 1.
The required materials to construct a 3D-printed recording chamber and the entire recording stage, complete with suggested vendors, the quantity of each item, and the price for the necessary quantity
Item description Vendor Quantity Price 1.75 mm PLA Amazon 351 g $6.31 M6 20 mm flat screw Cheap stair parts 1 $0.38 M6 10 mm screw Accu 4 $2.40 M6 16 mm screw Accu 4 $2.32 M6 nuts Accu 5 $1.55 M6 washers Accu 5 $1.65 24 * 24 mm coverslip AmScope 1 $0.07 Polyethylene tubing Thomas Specific 10 $0.02 Total for one system $14.70 - Table 2.
The required materials to construct a 3D-printed holding chamber, complete with suggested vendors, the quantity of each item, and the price for the necessary quantity
Item description Vendor Quantity Price 1.75 mm PLA Amazon 27 g $0.49 Unbleached cheesecloth Amazon 8.5 * 8.5 cm $0.09 Total $0.58
Figure 1-1
3D print and CAD files for the recording stage, recording chamber, and tissue holding chamber. STL and F3D files for download, adjustment, and printing of the recording stage, recording chamber, and tissue holding chamber. Download Figure 1-1, ZIP file.
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