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Research ArticleResearch Article: Negative Results, Sensory and Motor Systems

Human NGF “Painless” Ocular Delivery for Retinitis Pigmentosa: An In Vivo Study

Debora Napoli, Noemi Orsini, Giulia Salamone, Maria Antonietta Calvello, Simona Capsoni, Antonino Cattaneo and Enrica Strettoi
eNeuro 18 September 2024, 11 (9) ENEURO.0096-24.2024; https://doi.org/10.1523/ENEURO.0096-24.2024
Debora Napoli
1CNR Neuroscience Institute, Pisa 56124, Italy
2Regional Doctorate School in Neuroscience, University of Florence, Italy
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  • ORCID record for Debora Napoli
Noemi Orsini
1CNR Neuroscience Institute, Pisa 56124, Italy
2Regional Doctorate School in Neuroscience, University of Florence, Italy
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Giulia Salamone
1CNR Neuroscience Institute, Pisa 56124, Italy
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Maria Antonietta Calvello
3Bio@SNS Laboratory of Biology, Scuola Normale Superiore, Pisa, Italy
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Simona Capsoni
4Section of Human Physiology, Department of Neuroscience and Rehabilitation, University of Ferrara, Ferrara 44121, Italy
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Antonino Cattaneo
3Bio@SNS Laboratory of Biology, Scuola Normale Superiore, Pisa, Italy
5Rita Levi-Montalcini European Brain Research Institute (EBRI), Roma 00161, Italy
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Enrica Strettoi
1CNR Neuroscience Institute, Pisa 56124, Italy
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Article Information

DOI 
https://doi.org/10.1523/ENEURO.0096-24.2024
PubMed 
39293937
Published By 
Society for Neuroscience
History 
  • Received February 29, 2024
  • Revision received May 29, 2024
  • Accepted June 3, 2024
  • Published online September 18, 2024.
Copyright & Usage 
Copyright © 2024 Napoli et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Debora Napoli1,2,
  2. Noemi Orsini1,2,
  3. Giulia Salamone1,
  4. Maria Antonietta Calvello3,
  5. Simona Capsoni4,
  6. Antonino Cattaneo3,5 and
  7. Enrica Strettoi1
  1. 1CNR Neuroscience Institute, Pisa 56124, Italy
  2. 2Regional Doctorate School in Neuroscience, University of Florence, Italy
  3. 3Bio@SNS Laboratory of Biology, Scuola Normale Superiore, Pisa, Italy
  4. 4Section of Human Physiology, Department of Neuroscience and Rehabilitation, University of Ferrara, Ferrara 44121, Italy
  5. 5Rita Levi-Montalcini European Brain Research Institute (EBRI), Roma 00161, Italy
  1. Correspondence should be addressed to Enrica Strettoi at enrica.strettoi{at}in.cnr.it.
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Author contributions

  1. Author contributions: D.N., S.C., A.C., and E.S. designed research; D.N., N.O., G.S., M.A.C., and S.C. performed research; D.N., N.O., G.S., S.C., A.C., and E.S. analyzed data; M.A.C. contributed unpublished reagents/analytic tools; D.N., A.C., and E.S. wrote the paper.

Disclosures

  • The authors declare no competing financial interests.

  • We thank Martina Biagioni and Beatrice Di Marco for their valuable help in experimental parts and Alexia Tiberi and Giulia Borgonovo for testing the biological efficacy of hNGFp on the brain. We thank Francesca Biondi, Elena Novelli, and Renzo Di Renzo for their expert technical assistance. This study was supported by the National Recovery and Resilience Plan, Mission 4 Component 2 Investment 1.4–Ministry of University and Research (MUR) Call No. 3277. Project Code ECS_00000017 MUR Directorial Decree No. 1055, 23-06-2022, “Tuscany Health Ecosystem–THE.” The research was carried out also with the contribution of the research group linked to Velux Foundation (CH), GA No. 1236.

Funding

  • Tuscany Ecosystem _THE_; National Recovery Resiliency Plan, Mission 4, Component 2, Investiment 1.4- Ministry of University and Research-MUR

    Project Code ECS_00000017; MUR Directorial Decree n.1055
  • Velux Stiftung (Velux Foundation)

    Project n. 1236

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eneuro: 11 (9)
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September 2024
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Human NGF “Painless” Ocular Delivery for Retinitis Pigmentosa: An In Vivo Study
Debora Napoli, Noemi Orsini, Giulia Salamone, Maria Antonietta Calvello, Simona Capsoni, Antonino Cattaneo, Enrica Strettoi
eNeuro 18 September 2024, 11 (9) ENEURO.0096-24.2024; DOI: 10.1523/ENEURO.0096-24.2024

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Human NGF “Painless” Ocular Delivery for Retinitis Pigmentosa: An In Vivo Study
Debora Napoli, Noemi Orsini, Giulia Salamone, Maria Antonietta Calvello, Simona Capsoni, Antonino Cattaneo, Enrica Strettoi
eNeuro 18 September 2024, 11 (9) ENEURO.0096-24.2024; DOI: 10.1523/ENEURO.0096-24.2024
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Keywords

  • blindness
  • cell survival
  • inherited retinal degeneration
  • microglia
  • neurotrophic factors
  • photoreceptors

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