Article Figures & Data
Figures
- Figure 1.
Controlled food and water intake and behavioral measures. A, Five different experimental schedules for the control of food and water intakes: top row, regular feeding condition for daily allocation of the regular dry meal and water (Reg in B); second row, blood test condition for collecting blood for testing the influence of hunger and thirst (20 h in B); middle row, intake test condition to measure the food and water intake without anesthesia (test in C); fourth row, control condition for evaluating thirst to measure the effect of dry meal intake on water intake (con1 in C); bottom row, control condition to precheck whether 17 h of no food access was enough to induce the hunger, compared with the 20 h (17 h in B). The controlled food and water access is initiated on the day before the test day, at 14:00, except Control 2 condition. B, The amount of food intake in four monkeys during the blood test condition (20 h), Control 2 (17 h), and regular feeding with a constant dry meal applied at 11:00 (Reg). C, Amount of water intake in four monkeys during the intake test (test) and control Condition 1 (con1). In B, the result from monkey Y23 was not demonstrated because his blood data were not collected.
- Figure 2.
Blood ghrelin and osmolality levels before and after dry meal intake. A, The box plot of blood ghrelin levels in the four monkeys before (pre) and after (post) consuming the regular dry meal. B, The box plot of blood osmolality levels before (pre) and after (post) the consumption of regular dry meals in four monkeys. In A and B, the mean is indicated by a cross. Consecutive measurements are obtained daily using a line.
- Figure 3.
No consistent relationship between the changes in blood ghrelin and osmolality levels. A, No significant relationship observed between changes in blood ghrelin and osmolality levels before (pre) and after (post) the food intake. The dashed line represents the regression slope. B, Two consecutive measurements per day are represented by dotted black lines. The thick gray and orange lines indicate the regression lines for the data in the pre- and postperiods, respectively.
- Figure 4.
Amounts of food and water intake without anesthesia. A, The amount of the dry meal intake during the 1.5 h of the food intake test. B, The increase in water intake amount during the 1.5 h of the food intake test compared with that during control Condition 1, in which no food was provided. C, No significant relationship observed between the increase in water intake compared with control Condition 1 (vertical axis) and the amount of the food intake during the intake test (horizontal axis). The dashed line represents the regression slope.
- Figure 5.
Assessing the influence of plasma concentration on measuring optical density. Plots of the measured optical density (vertical axis) against standard concentrations. Each point is obtained from the average of two wells: no plasma (black), 5% plasma (blue), and 10% plasma (green). The colored dotted lines indicate a linear fit to the data. R2 are indicated.
In this issue
