Abstract
The integration of spatial information in the mammalian dentate gyrus (DG) is critical to navigation. Indeed, DG granule cells (DGCs) rely upon finely balanced inhibitory neurotransmission in order to respond appropriately to specific spatial inputs. This inhibition arises from a heterogeneous population of local GABAergic interneurons (INs) that activate both fast, ionotropic GABAA receptors (GABAAR) and slow, metabotropic GABAB receptors (GABABR), respectively. GABABRs in turn inhibit pre- and postsynaptic neuronal compartments via temporally long-lasting G-protein-dependent mechanisms. The relative contribution of each IN subtype to network level GABABR signal setting remains unknown. However, within the DG, the somatostatin (SSt) expressing IN subtype is considered crucial in coordinating appropriate feedback inhibition on to DGCs. Therefore, we virally delivered channelrhodopsin 2 to the DG in order to obtain control of this specific SSt IN subpopulation in male and female adult mice. Using a combination of optogenetic activation and pharmacology, we show that SSt INs strongly recruit postsynaptic GABABRs to drive greater inhibition in DGCs than GABAARs at physiological membrane potentials. Furthermore, we show that in the adult mouse DG, postsynaptic GABABR signaling is predominantly regulated by neuronal GABA uptake and less so by astrocytic mechanisms. Finally, we confirm that activation of SSt INs can also recruit presynaptic GABABRs, as has been shown in neocortical circuits. Together, these data reveal that GABABR signaling allows SSt INs to control DG activity and may constitute a key mechanism for gating spatial information flow within hippocampal circuits.
Significance Statement
GABAergic interneurons provide powerful inhibition to cortical circuits, by directly regulating the activity of other neurons through metabotropic GABAB receptors. While much is known about the basic properties of GABAB receptor signaling, current knowledge of the relative contribution made by specific interneuron subpopulations to this inhibitory neurotransmission mechanism is less well understood. Our results help address this knowledge gap by showing that the somatostatin-expressing interneuron subpopulation provide powerful GABAB receptor-mediated feedback inhibition in the mouse dentate gyrus. Furthermore, GABAB receptor activation in dentate gyrus was found to be tightly regulated by neuronal GABA uptake, and not astrocytes, thus providing self-regulated feedback inhibition. Together, these data provide novel insights into cell type-specific GABAB receptor-mediated control of dentate gyrus circuitry.
Introduction
Spatial information processing in the mammalian dentate gyrus (DG) requires dynamic and inhibitory GABAergic neurotransmission from local interneurons (INs) to control the timing of synaptic information arising from entorhinal cortex input (Pernía-Andrade and Jonas, 2014) in addition to controlling the excitability of dentate granule cells (DGCs; Hainmueller and Bartos, 2020). In the DG, INs release GABA from axons that are aligned in a layer-specific manner (Degro et al., 2022), which in turn activates GABA receptors found on both DGC and IN pre- and postsynaptic membranes (Kulik et al., 2018). In DGCs, the mechanisms subserving GABAA receptor (GABAAR)-mediated inhibitory influence on circuit function are well described (Bartos et al., 2001; Vida et al., 2006). While temporally slow, metabotropic GABAB receptors (GABABRs) have been described in DGCs (Mott et al., 1993, 1999; Degro et al., 2015), their activation mediated by different IN subtypes is less well understood.
GABABRs activate Kir3 channels (Bettler et al., 2004) and inhibit Ca2+ channels in dendrites and axons (Vigot et al., 2006). This modulation pattern shows cell- and region-specific differences, including in the DG (Mott and Lewis, 1991; Mott et al., 1993, 1999; Piguet, 1993; Brucato et al., 1995; Lüscher et al., 1997; Booker et al., 2013, 2017a, 2018, 2020; Foster et al., 2013; Degro et al., 2015). GABABRs are typically located outside of the synaptic cleft (Kulik et al., 2003). They are activated by GABA spillover, which allows for coordination of circuit activity over hundreds of milliseconds (Isaacson et al., 1993; Scanziani, 2000; Gonzalez-Burgos et al., 2009). This heterosynaptic nature suggests that INs can potentially activate neuronal GABABRs independently of direct synaptic connections (Scanziani, 2000; Degro et al., 2015) to regulate wider circuit function over longer timescales.
Indeed, some INs (e.g., neurogliaform cells—NGFCs) can recruit GABABR in a unitary manner (Thomson and Destexhe, 1999; Tamás et al., 2003; Price et al., 2005, 2008; Oláh et al., 2009). However, neuronal circuits rarely function in isolation and rather require coordinated activation of many neurons (Klausberger et al., 2003). INs that express somatostatin (SSt) are readily recruited by active circuits and provide powerful feedback inhibition over multiple timescales in both hippocampus and neocortex (Pangalos et al., 2013; Urban-Ciecko et al., 2015; Booker et al., 2020; Honoré et al., 2021). In the DG, SSt INs are located in the hilus (Savanthrapadian et al., 2014; Booker and Vida, 2018) where they release GABA onto the distal dendrites of granule cells (GCs), aligned to entorhinal cortex (EC) inputs (Booker and Vida, 2018; Degro et al., 2022) to dynamically control DG circuit activity (Hainmueller et al., 2024). Indeed, the axonal output of SSt INs very closely aligns with the highest expression of GABABRs and effector Kir3 channels on DGC dendrites (Degro et al., 2015). This indicates that SSt INs are ideally positioned to exert powerful GABABR inhibition on DGCs. Evidence does exist for SSt INs to recruit pre- and postsynaptic GABABRs (Urban-Ciecko et al., 2015; Gonzalez et al., 2018; Kanigowski et al., 2023). However, the mechanisms by which SSt INs recruit spillover of GABA to activate GABABRs, whether sex differences exist in signaling, or how presynaptic receptors modulate DG function remain unexplored.
In this study we hypothesize that SSt INs can activate pre- and postsynaptic GABABRs in the mouse DG and that this activation is tightly regulated by GABA uptake mechanisms. To test this, we virally transfected channelrhodopsin 2 into the DG of mice expressing Cre-recombinase under the SSt promotor channel. Using ex vivo whole-cell electrophysiology, we show that SSt INs recruit a significant postsynaptic GABABR-mediated current in DGCs. DG GABABR signaling displayed some sex dependence and was predominantly regulated by neuronal GABA uptake. Finally, we confirm that SSt INs can recruit presynaptic GABABRs to regulate neurotransmitter release, as previously described in neocortex.
Materials and Methods
Animals
Electrophysiological experiments were performed in acute slices prepared from adult (3–7-month-old) transgenic mice of both sexes expressing Cre-recombinase under the SSt promoter (SSttm2.1(cre)Zjh/J; Jackson Laboratory). All mice were housed in groups of 2–4 prior to surgery, maintained on a 12 h light/dark cycle. Under anesthesia (2% isoflurane), either unilateral or bilateral injections of AAV containing channelrhodopsin 2 (ChR2), and YFP coding regions between inverted incompatible tandem loxP sites (AAV2-FLEX-EF1a-DIO-hChR2(H134)-EYFP, ≥7 × 1012 vg/ml, Addgene) were made in to the DG (coordinates relative to the bregma: −2 mm posterior, 1.5 mm lateral and 1.5 mm ventral from brain surface; 500 nl volume). Slice recordings were performed ∼2 weeks following viral injection (12–17 d, median 14 d) at zeitgeber time 2–3. All animal procedures were performed in accordance with United Kingdom Home Office (ASPA 1986) and the University of Edinburgh animal care committee regulations.
Brain slice preparation and recording
Mice were sedated with isoflurane, anesthetized with sodium pentobarbital (20 mg/kg), and then transcardially perfused with ice-cold, carbogenated (95% O2/5% CO2) sucrose artificial cerebrospinal fluid (sucrose-ACSF, in mM: 87 NaCl, 2.5 KCl, 25 NaHCO3, 1.25 NaH2PO4, 25 glucose, 75 sucrose, 7 MgCl2, 0.5 CaCl2, 1 Na-pyruvate, 1 Na-ascorbate), and then the brain was rapidly dissected. Coronal hippocampal slices (300 μm) were cut on a vibratome (VT1200s, Leica) and stored in sucrose-ACSF at 35°C for 30 min and then at RT until recording, as previously described (Oliveira et al., 2021).
For recording, slices were placed in a submerged recording chamber, perfused with carbogenated ACSF (in mM: 125 NaCl, 2.5 KCl, 25 NaHCO3, 1.25 NaH2PO4, 25 glucose, 1 MgCl2, 2 CaCl2, 1 Na-pyruvate, 1 Na-ascorbate) at 6–8 ml/min maintained at near physiological temperatures (32 ± 1°C). Slices were visualized with IR-DIC with an upright microscope (SliceScope, Scientifica) with a 40× water-immersion objective lens (NA 0.8). Whole-cell patch-clamp recordings were accomplished using a MultiClamp 700B amplifier (Molecular Devices) with recording pipettes pulled from borosilicate glass capillaries (1.5 mm outer/0.86 mm inner diameter, Harvard Apparatus) on a horizontal electrode puller (P-97, Sutter Instruments). Pipettes were filled with intracellular solution that gave pipette resistances of 3–7 MΩ. In all recordings series resistance (Rs) was monitored, which was not compensated in voltage clamp. In current-clamp mode, the bridge was fully balanced. All signals were filtered online at either 2 kHz (voltage-clamp) or 5 kHz (current-clamp) using a two-pole Bessel filter and digitized at 20 kHz (Digidata 1550B, Axon Instruments) using pClamp 11 (Axon Instruments). Data were analyzed off-line using the open source Stimfit software package (Guzman et al., 2014). Neurons were rejected from further analysis when VM > −50 mV, if APs failed to overshoot 0 mV, initial Rs exceeded 30 MΩ, or Rs changed by >20% in the course of the recording.
DGCs were selected for recording on the basis of having small, round somata located in the GCL. For GABABR IPSC recordings, electrodes were filled with an K-gluconate intracellular solution (in mM: 142 K-gluconate, 4 KCl, 2 MgCl2, 0.2 EGTA, 10 HEPES, 2 Na2-ATP, 0.3 Na2-GTP, 10 Na2-phosphocreatine, 0.1% biocytin, pH 7.35, 290–310 mOsm). DGCs were first electrophysiologically characterized based on their response to a family of hyper- to depolarizing current injections (500 ms duration; −500 to 500 pA in 100 pA steps) from resting VM. Combined optogenetic and electrical stimulation experiments were performed in ACSF containing NBQX and AP-5 (10 and 50 µM, respectively) in voltage clamp at −60 mV. Optogenetic stimulation was performed using 473 nm LED light (CooLED) delivered to the whole field of view centered on the outer molecular layer (oML). Brief (5 ms) pulses of light of varying intensity (0–50% LED power) were used to deliver to the slice either singly or in trains of five stimuli at 50 Hz. For electrical stimulation, bipolar twisted Ni:Chrome electrodes were placed in the oML (∼500 μm distal to the recorded cell) and IPSCs elicited by five stimuli at 200 Hz (200 μs, 50 V). Following initial characterization of compound IPSCs (5–10 sweeps), 10 μM gabazine (SR-95,531) was bath applied to block GABAAR-mediated currents, and then a further 15 IPSCs were collected (at 20 s intervals). Following this baseline recording, 20 μM tiagabine, 100 μM SNAP-5,114, or 5 μM CGP-55,845 were applied to the bath. In a subset of recordings, slices from the contralateral hemisphere, which lacked strong ChR2-YFP expression, were recorded for electrical IPSCs only. In these recordings, following IPSC characterization 10 μM R-baclofen (a selective GABABR agonist) was bath applied for 5 min, followed by 5 μM CGP-55,845 for 5 min. In a subset of cells, we assessed the reversal potential of GABABR IPSCs following baseline optogenetic and electrical IPSC recordings. For this, the DGCs were voltage clamped at a variety of membrane potentials (−40 to −100 mV) and three IPSCs elicited at each level. Holding potentials were adjusted for the liquid junction potential, measured at 12 mV. For all IPSCs, the amplitude was measured over a 10 ms average window. IPSC kinetics was measured from average IPSCs. A total of 20–80% IPSC rise time was measured from the prestimulus baseline. Decay time constant was estimated from the fit of a monoexponential curve fitted to the average IPSC.
For presynaptic experiments, DGCs were recorded at −70 mV voltage-clamp using a Cs-gluconate–based internal solution (in mM: 140 Cs-gluconate, 3 CsCl, 0.2 EGTA, 10 HEPES, 2 Na2-ATP, 2 Mg-ATP, 0.3 Na2-GTP, 10 Na2-phosphocreatine, 5 QX-314.Cl, 0.1% biocytin, pH 7.35, 290–310 mOsm) using ACSF containing 10 μM gabazine. Excitatory postsynaptic currents (EPSCs) were elicited by placing a bipolar, twisted Ni:Chrome wire placed in the oML, and trains of stimuli delivered at either 20 or 50 Hz (5 or 13 stimuli, respectively). Stimulus strength (mean, 22.7 V; range, 5–50 V) was chosen to generate a monosynaptic EPSC (mean, −127 pA; range, −27 to −356 pA). For co-activation of SSt INs, optogenetic stimulation 473 nm LED light was delivered at 20% maximum power. Five-millisecond optogenetic stimulation was delivered concomitant with each electrical stimulus. Following wash-in of the internal solution, 10 EPSCs were collected for each frequency stimulus train (10 s intervals) with or without optogenetic activation, then 5 μM CGP-55,845 was bath applied for 5 min, and then trains of stimuli were delivered again. In a subset of recordings, gabazine was omitted from the ACSF and neurons recorded at 0 mV, to measure the monosynaptic IPSC resulting from ChR2 activation, in the absence of electrical stimulation. In these recordings, a minimum of 30 sweeps were collected (10 s intervals), and then 5 μM CGP-55,845 was applied to the bath. For experiments blocking Gi/o activation, slices were incubated in recording ACSF containing 500 µg/ml pertussis toxin for 2–3 h prior to transfer to the recording chamber. For analysis, the amplitude of each EPSC was measured as the peak response (2.5 ms average window) relative to the prestimulus baseline. Short-term plasticity (STP) is plotted as the amplitude of EPSCs in the stimulus train normalized to the first EPSC.
Visualization, imaging, and reconstruction of the recorded neurons
Following recording, neurons were sealed with outside-out patches and fixed in 4% paraformaldehyde diluted in 0.1 M PB overnight (O/N) at 4°C. Immunohistochemistry was performed as previously described (Booker et al., 2014). Briefly, slices were washed with phosphate-buffered saline (PBS; 0.025 M PB and 0.9% NaCl) and then blocked with 10% normal goat serum (NGS) with 0.5% Triton X-100 and 0.05% NaN3 diluted in PBS for 1 h at RT. Slices were then incubated for 72 h in a PBS solution containing 5% NGS, 0.3% Triton X-100, and 0.05% NaN3 and primary antibodies against SSt (rabbit polyclonal, 1:500, Peninsula Laboratories), at 4°C. Slices were then washed in PBS for an hour and then incubated with fluorescently conjugated secondary antibodies raised against rabbit (goat anti-rabbit Alexa Fluor 568; 1:500, Thermo Fisher Scientific) as well as fluorescently conjugated streptavidin (Alexa Fluor 633; 1:500, Thermo Fisher Scientific) to visualize recorded neurons, in a PBS solution containing 3% NGS, 0.1% Triton X-100%, and 0.05% NaN3 overnight at 4°C. Slices were rinsed in PBS and then 0.1 M phosphate buffer containing 1 μg/ml DAPI and mounted on glass slides (Fluoromount-G, SouthernBiotech). Filled neurons were imaged with a laser scanning confocal microscope (SP8, Leica) using a 20× objective lens (NA 0.75). For quantification of SSt/ChR2-YFP overexpression, z-axis stacks of images (2,0482 pixels, 1 μm steps) cover the extent of the slice (1 slice/injected hemisphere/mouse). Selected, example cells were imaged for confirmation of cell type. All image analysis was performed using FIJI (ImageJ, http://fiji.sc).
Statistical analysis
All data is shown from cell average replicates as 1–2 cells per condition being collected per biological replicate (individual mouse); thus it was not possible to obtain an estimate of intra-animal variability. All graphs display data generated from individual cells, except for Fig. 1B,E, which display animal average data. Mice of either sex were used throughout. As all mice expressed Cre-recombinase under the SSt-cre promotor, and ChR2-YFP expression was assessed prior to recording, it was not possible to be blind to virus expression. Throughout, all data is shown as the mean ± SEM (except for Table 1). Statistical analysis was performed with GraphPad Prism (GraphPad Software). Analysis of unpaired data was performed with Student's t test or Mann–Whitney U tests depending on whether data were normally distributed (D’Agostino–Pearson test). Group data were compared with two-way ANOVA tests, combined with Sidak post-tests. Statistical significance was assumed if p < 0.05.
Results
DG SSt INs directly recruit multiple GABA receptors
A key primary aim of this study was to determine whether SSt INs are capable of activating postsynaptic GABABRs. To address this, we expressed channelrhodopsin 2 (ChR2-YFP) in SSt INs in the dorsal DG and then prepared coronal brain slices ∼14 d after AAV injection (range, 12–19 d). Viral expression was confirmed by the presence of YFP-positive somata restricted to the hilus and with dense axon collaterals in molecular layers (MLs; Fig. 1A).
When compared with immunohistochemical labeling, we observed that 64.0 ± 6.1% of SSt neurons expressed ChR2-YFP (n = 7 mice; Fig. 1B). This labeling was highly specific, with few (3.2 ± 1.2%) neurons expressing ChR2-YFP, but without detectable SSt. To directly measure GABABR currents, we performed whole-cell patch-clamp recordings from identified DGCs from male (n = 9 mice) and female (n = 12 mice; Fig. 1C). DGCs did not display sex-specific differences in action potential discharge (Fig. 1D,E) or most other basal electrophysiological properties, with the exception of action potential threshold (Table 1).
SSt INs possess axons primarily ramifying in the oML (Hosp et al., 2014; Yuan et al., 2017; Booker and Vida, 2018; Degro et al., 2022) which coaligns with high-density GABABR and Kir3 channel expression (Degro et al., 2015). In whole-cell patch-clamp recordings performed in DGCs from −60 mV, we optogenetically stimulated slices over the oML to recruit dendritic GABAergic currents originating from SSt IN activation. Single stimuli (5 ms duration) resulted in 13.6 ± 2.2 pA currents, which had a fast rise, slow decay—consistent with GABAAR activation at −60 mV (ER[Cl−] ≈ −72 mV; Fig. 1F). Trains of five stimuli (50 Hz) resulted in both fast and slow IPSCs, the latter of which lasted several hundred milliseconds and remained following bath application of the GABAAR antagonist gabazine (10 μM). The peak amplitude of fast and slow IPSCs largely tracked each other with increasing stimulation (Fig. 1G), albeit with smaller shoulder currents [F(1,80) = 41.68; p < 0.0001; two-way ANOVA (current type); n = 9 cells from 3 mice]. However, when charge of IPSCs was measured, it was apparent that GABABR-mediated currents from SSt IN optogenetic stimulation were significantly larger (as measured from the soma at −60 mV) than for GABAARs [F(1,80) = 39.85; p < 0.0001; two-way ANOVA (current type); Fig. 1H].
GABA release from SSt INs recruits GABABR-Kir3 currents and which is regulated by neuronal uptake mechanisms
To pharmacologically identify the slow IPSCs, we optogenetically stimulated slices (5 × 50 Hz) while recording interleaved electrically evoked IPSCs resulting from oML electrical stimulation (5 × 200 Hz stimuli, 50 V amplitude). Both optogenetic and electrical stimulated IPSCs displayed a fast and slow IPSC. Fast IPSCs were mediated by GABAAR due to their sensitivity to gabazine, leaving only slow outward currents which were blocked by the selective antagonist CGP-55,845 (CGP; 5 μM), confirming they were mediated by GABABRs (Fig. 2A). In some cells, GABABR-mediated IPSCs were observed even in the absence of SSt IN-mediated GABAAR responses (2 out of 28 cells), indicating a heterosynaptic nature of GABABR activation (Fig. 2B). GABABR-mediated optogenetic IPSCs had amplitudes of 4.95 ± 0.59 pA, with slow 20–80% rise (45.4 ± 4.3 ms) and long decay time constants (173.2 ± 11.7 ms; n = 31 cells from 15 mice; Fig. 2C). We observed no difference in amplitude (U(27) = 74; p = 0.285; Mann–Whitney test) or rise time (t(22) = 0.616; p = 0.545; Student's two-tailed t test) between male (n = 14 cells from 8 mice) and female mice (n = 14 cells from 7 mice). GABABR decay time constants were typically longer in female mice, compared with males, albeit not significantly so (U(25) = 56; p = 0.159; Mann–Whitney test). We confirmed that SSt IN-driven GABABR-mediated activation led to Kir3 channel opening, as slow IPSCs had a reversal potential of −93.2 mV and displayed clear inward rectification depolarized potentials (n = 6 cells from 5 mice; Fig. 2D). Electrical stimulation of oML produced similar GABABR-mediated IPSCs, albeit they were substantially larger (Fig. 2E). Notably, GABABR-mediated IPSCs evoked by electrical stimulation in female mice (n = 17 cells from 8 mice) had an average decay time constant of 187.2 ± 42.3 ms, which was significantly slower than that of male mice (150.1 ± 4.8 ms; n = 18 cells from 8 mice; U(34) = 58; p = 0.0012; Mann–Whitney test). Whole-cell GABABR-mediated responses in DGCs were activated by the agonist baclofen (10 μM), blocked by CGP, and were sensitive to pertussis toxin; which blocks Gi/o activation (Fig. 2F). Indeed, pertussis toxin pretreatment reduced the average peak baclofen current by 72% (U(14) = 1; p = 0.0029; Mann–Whitney test; Fig. 2G). Comparison of electrically evoked GABABR IPSCs and peak baclofen-mediated whole-cell currents revealed that direct stimulation of endogenous GABA release in oML (recruiting all dendritic targeting INs) activated all DGC receptors (U(36) = 123; p = 0.523; Mann–Whitney test; Fig. 2H). Together, these data confirm that SSt INs recruit a substantial proportion of native GABABRs, which are largely similar between sexes—but with subtle changes in decay kinetics.
GABABR activation is driven by heterosynaptic spillover of GABA (Isaacson et al., 1993; Scanziani, 2000), which may be mediated by neuronal (Gonzalez-Burgos et al., 2009; Gonzalez et al., 2018) or astrocytic uptake mechanisms (Matos et al., 2018; Shen et al., 2022). To determine if spillover of GABA contributes to GABABR signaling in DGCs, we blocked transporters located on either neurons (GAT-1) or astrocytes (GAT-3), with tiagabine or SNAP-5,114, respectively (Fig. 3A,B). Tiagabine bath application substantially increased the amplitude of optogenetic SSt IN mediated (247 ± 32% of control) and electrically-evoked GABABR-mediated IPSCs (183 ± 13% of control; Fig. 3C). This was mirrored in a substantial increase in GABABR-mediated charge transfer of SSt IN (405 ± 71% of control; n = 14 cells from 11 mice) and electrically evoked responses (414 ± 38% of control, Fig. 3D). In contrast, bath application of SNAP-5,114 increased IPSC amplitude in response to optogenetic stimulation (134 ± 10% of control; n = 8 cells from 6 mice) and electrical stimulation (170 ± 7% of control; p = 0.008; Wilcoxon matched-pairs test), but to a lesser degree than for tiagabine [F(1,38) = 12.98; p = 0.0009; two-way ANOVA (drug); Fig. 3C]. A similar pattern was observed for charge transfer, with tiagabine displaying much stronger IPSC boosting than SNAP-5,114 [F(1,39) = 22.1; p < 0.0001 (drug); Fig. 3D]. Tiagabine prolonged the 20–80% rise time of GABABR-mediated IPSCs, irrespective of whether from SSt INs or from the total population of INs (Fig. 3E), while SNAP-5,114 had no effect on onset kinetics (Fig. 3F). Likewise, tiagabine prolonged the decay time constants of all GABABR-mediated IPSCs (Fig. 3G), while SNAP-5,114 did not (Fig. 3H). These data reveal that in DG circuits, heterosynaptic spillover of GABA is tightly regulated by neuronal reuptake (with a lesser role of astrocytes) to regulate GABABR activation.
Presynaptic GABABRs at EC inputs to DG are recruited by SSt INs to control inputs
GABABRs provide powerful presynaptic control to excitatory and inhibitory afferents by inhibiting Ca2+ influx (Kulik et al., 2018), which may be controlled by SSt IN activation in neocortex (Urban-Ciecko et al., 2015; Kanigowski et al., 2023). To determine whether SSt-dependent GABA release can control integration of synaptic information in DG, we next simultaneously stimulated perforant-path afferents with optogenetic stimulation to test the effect of SSt IN-mediated GABABR inhibition on putative spatial inputs.
In the presence of gabazine to block potential effects of GABAARs, electrical stimulation was delivered at 20 Hz (5 stimuli) or 50 Hz (13 stimuli; Fig. 4A) to the oML while recording DGCs at −70 mV with a Cs+-based internal solution to block postsynaptic K+ currents. EPSCs evoked by perforant-path stimulation had amplitudes of 162.3 ± 29.0 pA. Amplitude of the first EPSC was not affected by either co-stimulation of oML and optogenetic activation of SSt INs (p = 0.520; Wilcoxon signed-rank test) or CGP application (p = 0.891; Wilcoxon signed-rank test; Fig. 4B), respectively, thus indicating tonic heterosynaptic GABABR activation under baseline conditions. To induce STP of EPSCs, 20 Hz stimulation of perforant path inputs was performed in 15 cells from 11 mice. When concomitant optogenetic stimulation was given with oML stimulation, this facilitation was significantly reduced [F(2,105) = 3.760; p = 0.027; two-way ANOVA (drug); Fig. 4C]. This suppression of EPSC amplitude was blocked by bath application of CGP-55,845 (CGP; 5 μM), confirming that presynaptic GABABRs were responsible for this STP suppression. In contrast, 50 Hz perforant-path stimulation initially resulted in facilitating STP, which returned to baseline levels after five EPSCs (n = 11 cells from 9 mice; Fig. 4D). Concomitant SSt IN optogenetic stimulation largely prevented this facilitation and resulted in sustained depression of repetitive EPSCs, which was blocked by subsequent application of CGP [F(2,351) = 9.657; p < 0.0001; two-way ANOVA (treatment)].
As GABABRs can contribute to autoreceptor-mediated control of SSt IN presynaptic release (Booker et al., 2020), we performed a subset of recordings at 0 mV with the same optogenetic stimuli frequencies but without electrical stimulation (Fig. 4E). When recorded at 20 Hz, SSt IN-mediated GABAAR-mediated IPSCs were minimally depressing, which revealed greater short-term depression following CGP bath application [F(1,188) = 39.3; p < 0.0001; two-way ANOVA (treatment); Fig. 4F]. Under control conditions, 50 Hz stimulation of SSt INs produced greater short-term depression, which was also exacerbated by CGP bath application [F(1,79) = 35.8; p < 0.0001; two-way ANOVA (treatment); Fig. 4G]. Pretreatment of slices with pertussis toxin fully prevented any CGP-dependent change in STP [F(1,62) = 0.68; p = 0.41; two-way ANOVA (treatment); Fig. 4H]. Comparison of the STP (IPSC 8–10) and GABAAR-mediated IPSC amplitude revealed that pertussis toxin itself occludes GABABR-dependent synaptic depression caused by CGP [F(1,19) = 4.9; p = 0.039; two-way ANOVA (pertussis toxin); Fig. 4J] and also occludes CGP-dependent enhancement of the first IPSC [F(1,18) = 4.6; p = 0.045; two-way ANOVA (CGP); Fig. 4K].
We found GABABRs activated by SSt INs contribute to the STP of perforant path inputs to the DG, as well as their own inhibitory strength. Our data indicate that SSt IN output is strongly regulated by tonic and activity-dependent autoreceptor activation, which require Gi/o protein activation. These data confirm that feedback SSt INs contribute to ongoing presynaptic inhibition of inputs to DG that likely contributes to gating of incoming spatial information.
Discussion
We provide direct evidence that SSt INs are a major contributor to GABABR inhibition of DG circuits, which is regulated by neuronal GABA uptake. Furthermore, we highlight the presence of sex differences in DGC excitability and GABABR signaling. Finally, we provide direct evidence that presynaptic GABABR activation by SSt INs controls the strength of incoming synaptic inputs from entorhinal cortex to the DG and by SSt INs themselves.
Hippocampal microcircuits are crucial for pattern separation of information, which requires sparse information transfer (Leutgeb et al., 2007; Schmidt-Hieber and Nolan, 2017). DG hilar INs (including SSt) are likely required for this process (Myers and Scharfman, 2009), but studies have largely focused on GABAAR signaling (Hefft and Jonas, 2005; Savanthrapadian et al., 2014; Guzman et al., 2021). DGCs, however, typically possess hyperpolarized membrane potentials that lead to fast and shunting inhibition (Bartos et al., 2001; Vida et al., 2006). GABABRs, in contrast, provide long-lasting, consistently hyperpolarizing and presynaptic inhibition to DGCs (Mott et al., 1993, 1999; Foster et al., 2013; Degro et al., 2015). Our data shows that feedback SSt INs contribute to GABABR-mediated inhibition and may serve as an internal brake to regulate DGC activity, controlled by DGCs themselves. SSt IN activation of presynaptic GABABRs has been shown in active neocortical circuits (Urban-Ciecko et al., 2015; Kanigowski et al., 2023). Our data confirm that such recruitment of GABABRs by SSt INs is a likely common circuit motif across brain regions. In contrast, the DGCs display sparse firing rates and are capable of strongly recruiting SSt INs (Yuan et al., 2017). Our data provides evidence that SSt INs are also capable of recruiting high levels of postsynaptic GABABR activation. Such inhibition within local circuits is postulated to contribute to the coherent timing of neuronal activity, particularly the nesting of theta, gamma, and ripple oscillations (Sik et al., 1997; Pangalos et al., 2013; Booker et al., 2020), which are sensitive to GABABR activation (D'Antuono et al., 2005). How SSt IN-mediated GABABR activation contributes to pattern separation and information coding in DG requires further investigation.
The source of GABA that drives GABABR-mediated signaling likely originates from dendritic targeting INs (Scanziani, 2000; Booker et al., 2013; Gonzalez et al., 2018). Our data show that SSt INs contribute a significant portion of this GABA, in line with previous reports (Gonzalez et al., 2018). Indeed, our measured ∼60% viral transfection efficiency and whole-cell baclofen currents suggest that SSt INs activate up to 20% of GABABRs in DGCs. This complements the GABA originating from NGFCs in DG, which give rise to unitary GABABR-mediated IPSCs (Armstrong et al., 2011) and are known to contribute more GABABR signaling than SSt INs (Gonzalez et al., 2018). However, SSt INs are strongly recruited by DGC activity (Yuan et al., 2017) and have higher numbers in DG than neurochemically defined IN populations, including NGFCs (Jinno and Kosaka, 2006; Armstrong et al., 2011). As such, in vivo, SSt INs in the DG may lead to greater GABABR activation to provide powerful feedback inhibition to DGCs on oML dendrites (Yuan et al., 2017). This result builds upon and extends previous findings suggesting a role for other INs, not just NGFCs, in producing GABABR currents in PCs (Isaacson et al., 1993; Scanziani, 2000; Booker et al., 2013; Gonzalez et al., 2018). Our current findings also add further weight to the suggestion that multiple sources of GABA-mediated volume transmission exist. Importantly, such GABA sources likely rely on circuit-wide activation of many IN subtypes simultaneously or sequentially. Given the feedback nature of SSt INs, and their high connectivity with DGCs and hilar mossy cells (Savanthrapadian et al., 2014; Yuan et al., 2017), it is probable that they are recruited during behavior and their activation of GABABRs contributes to sparsification of spatial information (Myers and Scharfman, 2009; Hainmueller et al., 2024), plausibly explaining why pharmacological manipulation of GABABRs can limit spatial memory formation (Brucato et al., 1995) and regulate DG spiking (Foster et al., 2013). GABABRs likely play a critical role in controlling DGC activity, as at physiological membrane potentials they are consistently hyperpolarizing and exert strong presynaptic control; unlike GABAARs, which due to the relatively hyperpolarized membrane potentials of DGCs, largely exert shunting inhibition (Vida et al., 2006). Thus, GABABR activation by SSt INs may contribute to circuit-level inhibition—particularly during exploration of novel environments (Hainmueller et al., 2024). We also show that GABABR-mediated currents from SSt INs are regulated near-exclusively by neuronal GABA uptake and not astrocytes. The extent to which this is true for GABAAR IPSCs and whether DG SSt INs display astrocyte-dependent adenosine regulation as in CA1 (Matos et al., 2018; Shen et al., 2022) remains unknown. However, the role of astrocytes in DG GABABR signaling is likely minimal given the failure of SNAP-5,114 to increase either amplitude or charge of SSt IN GABABR-mediated IPSCs. This may highlight differences in circuit function of SSt INs (Hainmueller et al., 2024) and/or the role of astrocytes across hippocampal subfields (Matos et al., 2018).
Despite a lack of difference in the majority of measured electrophysiological parameters, we found heterogeneity in the decay kinetics of GABABR-mediated IPSCs between male and female mice in IPSCs not originating from SSt INs. Neurogliaform INs contribute approximately twice the GABABR-mediated currents to DGCs, compared with SSt INs (Gonzalez et al., 2018). Sex-specific differences in NGFCs have been reported in other brain structures (Gegenhuber et al., 2022), which may indicate that this is the source of altered GABABR signaling. This adds to a growing body of literature highlighting sex-specific differences in GABABR signaling at the neuronal (De Velasco et al., 2015) and behavioral levels (Campbell et al., 2002; Jeanblanc et al., 2023). The specific mechanisms underlying GABABR kinetic modulation remain unclear; however, it is possible that altered expression of K-channel tetramerization domain proteins (KCTD) may be involved. In particular, KCTD12 confers longer GABABR decay times (Fritzius et al., 2017; Booker et al., 2017b). Recent evidence has shown that KCTD12 contributes to aggressive behavior between female and male Drosophila (Williams et al., 2014). Whether such a mechanism exists in mammals is currently unknown.
In summary, we show that SSt INs significantly activate pre- and postsynaptic GABABRs in in the adult mouse DG, GABA released from SSt INs is regulated in the extracellular space by neuronal uptake mechanisms, and sex-specific differences in GABABR kinetics exist. This highlights the importance of GABABRs in local circuit dynamics, particularly in the context of spatial information processing.
Footnotes
The authors declare no competing financial interests.
We thank the Rochefort Lab (University of Edinburgh) for providing experimental animals. We thank Darren Walsh and Nina Petrovic for helpful comments on the manuscript and the Wood, Kind, and Wyllie Labs for helpful discussions of initial data. Confocal imaging was performed in the IMPACT Imaging Facilities (CDBS, University of Edinburgh) with support from Dr. Anisha Kubasik-Thayil. This study was funded by the Simons Initiative for the Developing Brain (SFARI: 529085) and the RS McDonald Seedcorn Fund.
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